Protein interaction analysis of circadian clock protein
| Project/Area Number |
22370037
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
NAKATSU Toru 京都大学, 大学院・薬学研究科, 准教授 (50293949)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
|
|---|
| Keywords | 生体リズム / 時計タンパク質 / 生物時計 / 概日リズム / X線結晶構造解析 / 蛋白質間相互作用 / bHLHドメイン / PASドメイン |
|---|
| Research Abstract |
BMAL1 and CLOCK are the transcription factors of the circadian clock in mammals. The human BMAL1:CLOCK bHLH domain forms hetero or homo dimer and binds to E-box DNA. The hetero dimer of bHLH domain was more preferable to recognize the E-BOX DNA compared to the homo dimer based on the ITC analysis. Next we investigated the region related to the formation of the bHLH PAS-A domain of the human BMAL1:CLOCK hetero dimer. It is suggested that the bHLH domain is more important for the hetero dimer formation than the PAS-A domain.
|
Report
(4 results)
Research Products
(23 results)
-
-
-
-
-
-
-
-
[Presentation] Production of Crystalline SWCNT aggregates2012
Author(s)
Hikaru Kudo, Kazuhiro Yanagi, Kazutomo Suenaga, Haruka kobayashi, Taishi Takenobu, Yohei Yomogida, Kunio Hirata, Ryosuke Yoshimune, Toru Nakatsu
Organizer
フラーレンナノチューブグラフェン総合シンポジウム
Place of Presentation
東京大学(東京都)
Year and Date
2012-03-08
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
