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Regulatory mechanism of intracellular trafficking of ceramide

Research Project

Project/Area Number 22370054
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

HANADA KENTARO  国立感染症研究所, 細胞化学部, 部長 (30192701)

Co-Investigator(Renkei-kenkyūsha) KUMAGAI Keigo  国立感染症研究所, 細胞化学部, 主任研究官 (40443105)
YAMAJI Toshiyuki  国立感染症研究所, 細胞化学部, 主任研究官 (50332309)
Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywords膜輸送 / 輸送タンパク質 / 脂質 / セラミド / スフィンゴミエリン / リン酸化 / 小胞体 / ゴルジ体 / 脂質輸送 / タンパク質リン酸化 / ゲノム編集 / HeLa細胞 / クラミジア / スフィンゴ脂質 / 生体膜
Research Abstract

Lipid transfer proteins have been shown to mediate inter-organelle trafficking of various types of lipids in cells. However, mechanisms underlying the regulation of intracellular lipid trafficking remain poorly elucidated. In this research, we found i) phosphorylation at serine 315 of the CERT protein, which mediates transport of ceramide from the endoplasmic reticulum (ER) to the Golgi apparatus, strongly enhances the interaction of CERT with the ER-membrane protein VAP, and ii) this phosphorylation up-regulates ER-to-Golgi transport of ceramide. We previously showed that de-phosphorylation at another specific site of CERT up-regulates both activities for inter-membrane transfer of ceramide and Golgi-association of CERT. These results revealed that CERT-mediated ceramide trafficking is regulated by multiple protein kinase/phosphatase systems.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (70 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (17 results) (of which Peer Reviewed: 15 results) Presentation (46 results) (of which Invited: 14 results) Remarks (7 results)

  • [Journal Article] Phosphoregulation of CERT at serine 315 in the interaction with VAMP-associated protein (VAP) for inter-organelle trafficking of ceramide in mammalian cells2014

    • Author(s)
      Kumagai K, Kawano-Kawada M, and Hanada K
    • Journal Title

      J Biol Chem

      Volume: 289 Issue: 15 Pages: 10748-10760

    • DOI

      10.1074/jbc.m113.528380

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of HeLa cell mutants deficient in sphingolipid-related genes by using TALENs2014

    • Author(s)
      Yamaji T, and Hanada K
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 2 Pages: e88124-e88124

    • DOI

      10.1371/journal.pone.0088124

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Co-evolution of sphingomyelin and the ceramide transport protein CERT2014

    • Author(s)
      Hanada K
    • Journal Title

      Biochim Biophys Acta

      Volume: 1841 Issue: 5 Pages: 704-719

    • DOI

      10.1016/j.bbalip.2013.06.006

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A role for sphingomyelin-rich lipid domains in the accumulation of phosphatidylinositol-4,5-bisphosphate to the cleavage furrow during cytokinesis2014

    • Author(s)
      Abe M, Makino A, Hullin-Matsuda F, Kamijo K, Ohno-Iwashita Y, Hanada K, Mizuno H, Miyawaki A, and Kobayashi T
    • Journal Title

      Mol Cell Biol

      Volume: 32 Issue: 8 Pages: 1396-1407

    • DOI

      10.1128/mcb.06113-11

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 効率的な細胞内セラミド輸送の仕組みとその制御2014

    • Author(s)
      熊谷圭悟, 花田賢太郎
    • Journal Title

      医学の歩み

      Volume: 248 Pages: 1125-1131

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Structural basis for the Golgi association by the pleckstrin homology domain of the ceramide trafficking protein (CERT)2012

    • Author(s)
      Sugiki T, Takeuchi K, Yamaji T, Takano T, Tokunaga Y, Kumagai K, Hanada K, Takahashi H, and Shimada I
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 40 Pages: 33706-33718

    • DOI

      10.1074/jbc.m112.367730

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Limonoid compounds inhibit sphingomyelin biosynthesis by preventing CERT protein-dependent extraction of ceramides from the endoplasmic reticulum2012

    • Author(s)
      Hullin-Matsuda F, Tomishige N, Sakai S, Ishitsuka R, Ishii K, Makino A, Greimel P, Abe M, Laviad EL, Lagarde M, Vidal H, Saito T, Osada H, Hanada K, Futerman AH, and Kobayashi T
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 30 Pages: 24397-24411

    • DOI

      10.1074/jbc.m112.356733

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reconstitution assay system for ceramide transport with semi-intact cells2012

    • Author(s)
      Kumagai K, Nishijima M, and Hanada K
    • Journal Title

      Methods Cell Biol

      Volume: 108 Pages: 117-129

    • DOI

      10.1016/b978-0-12-386487-1.00006-7

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Limonoid compounds inhibit sphingomyelin biosynthesis by preventing CERT protein-dependent extraction of ceramides from the endoplasmic reticulum2012

    • Author(s)
      Francoise Hullin-Matsuda
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Issue: 29 Pages: 33706-33718

    • DOI

      10.1074/jbc.m112.344432

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chlamydia trachomatis co-opts GBF1 and CERT to acquire host sphingomyelin for distinct roles during intracellular development2011

    • Author(s)
      Elwell CA, Jiang S, Kim JH, Lee A, Wittmann T, Hanada K, Melancon P, and Engel JN
    • Journal Title

      PLoS Pathog

      Volume: 7 Issue: 9 Pages: e1002198-e1002198

    • DOI

      10.1371/journal.ppat.1002198

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transmembrane BAX inhibitor motif containing (TMBIM) family proteins perturb a trans-Golgi network enzyme, Gb3 synthase, and reduces Gb3 biosynthesis2010

    • Author(s)
      Yamaji T, Nishikawa K, and Hanada K
    • Journal Title

      J Biol Chem

      Volume: 285 Issue: 46 Pages: 35505-35518

    • DOI

      10.1074/jbc.m110.154229

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Real-time assay method of lipid extraction activity2010

    • Author(s)
      Sugiki T, Takahashi H, Nagasu M, Hanada K, Shimada I
    • Journal Title

      Anal Biochem

      Volume: 399 Issue: 2 Pages: 162-167

    • DOI

      10.1016/j.ab.2009.12.031

    • Related Report
      2013 Final Research Report
  • [Journal Article] Crystal structures of the CERT START domain with inhibitors provide insights into the mechanism of ceramide transfer2010

    • Author(s)
      Kudo N, Kumagai K, Matsubara R, Kobayashi S, Hanada K, Wakatsuki S, and Kato R
    • Journal Title

      J Mol Biol

      Volume: 396 Issue: 2 Pages: 245-251

    • DOI

      10.1016/j.jmb.2009.12.029

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular trafficking of ceramide by ceramide transfer protein2010

    • Author(s)
      Hanada K
    • Journal Title

      Proc Jpn Acad Ser B Phys Biol Sci

      Volume: 86 Pages: 426-437

    • NAID

      130000258738

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular trafficking of ceramide by ceramide transfer protein2010

    • Author(s)
      Kentaro Hanada
    • Journal Title

      Proceedings of the Japan Academy, Series B

      Volume: 86 Pages: 426-437

    • NAID

      130000258738

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transmembrane BAX inhibitor motif containing (TMBIM) family proteins perturbs a trans-Golgi network enzyme, Gb3 synthase, and reduces Gb3 biosynthesis2010

    • Author(s)
      Toshiyuki Yamaji
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 285 Pages: 35505-35518

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-time assay method of lipid extraction activity2010

    • Author(s)
      Toshihiko Sugiki
    • Journal Title

      Analytical Biochemistry

      Volume: 399 Pages: 162-167

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Introductory remarks for the session of membrane dynamics and Trafficking2014

    • Author(s)
      Hanada K
    • Organizer
      Gordon Research Conference on Glycolipid and Sphingolipid Biology
    • Place of Presentation
      Ventura, CA, USA
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] TALEN-mediated disruption of several sphingolipid-related genes in a HeLa cell line2014

    • Author(s)
      Yamaji T, and Hanada K
    • Organizer
      Gordon Research Conference on Glycolipid and Sphingolipid Biology
    • Place of Presentation
      Ventura, CA, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Introductory remarks for the session of Membrane Dynamics and Trafficking2014

    • Author(s)
      Kentaro Hanada
    • Organizer
      Gordon Research Conference on Glycolipid and Sphingolipid Biology
    • Place of Presentation
      Ventura, California, USA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] TALEN-Mediated Disruption of Several Sphingolipid-Related Genes in a HeLa cell line2014

    • Author(s)
      Toshiyuki Yamaji and Kentaro Hanada
    • Organizer
      Gordon Research Conference on Glycolipid and Sphingolipid Biology
    • Place of Presentation
      Ventura, California, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Molecular architecture of CERT for inter-organelle transport of ceramide at the ER-Golgi membrane contact sites2013

    • Author(s)
      Hanada K
    • Organizer
      The 14th International Membrane Research Forum
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] TALEN-Mediated Disruption of Sphingolipid-Related Genes in a HeLa Cell Line2013

    • Author(s)
      Yamaji T, and Hanada K
    • Organizer
      54th International Conference on the Bioscience of Lipids : Linking Transcription to Physiology in Lipidomics
    • Place of Presentation
      Bari, Italy
    • Related Report
      2013 Final Research Report
  • [Presentation] 細胞内セラミド輸送をつかさどるCERT の発見2013

    • Author(s)
      花田賢太郎
    • Organizer
      第6回セラミド研究会学術集会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] 遺伝生化学的手法を用いたスフィンゴ糖脂質研究2013

    • Author(s)
      山地俊之, 花田賢太郎
    • Organizer
      第86回日生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] 人工ヌクレアーゼTALEN を用いた種々のスフィンゴ脂質関連遺伝子の変異株作製2013

    • Author(s)
      山地俊之, 花田賢太郎
    • Organizer
      第32回日本糖質学会大会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] 人工ヌクレアーゼを利用したゲノム編集技術によるスフィンゴ脂質関連遺伝子破壊HeLa 細胞変異株パネル作成の試み2013

    • Author(s)
      山地俊之,花田賢太郎
    • Organizer
      第8回スフィンゴテラピー研究会
    • Place of Presentation
      加賀
    • Related Report
      2013 Final Research Report
  • [Presentation] 人工ヌクレアーゼを用いた種々のスフィンゴ脂質関連遺伝子の変異株作製2013

    • Author(s)
      山地俊之, 花田賢太郎
    • Organizer
      第55回日本脂質生化学会大会
    • Place of Presentation
      松島
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Structural biology revealed the serendipity of development of HPA-12, a potent inhibitor of intracellular trafficking of ceramide2013

    • Author(s)
      Kentaro Hanada
    • Organizer
      , FASEB Science Research Conference: Lysophospholipid and other Related Mediators - From Bench to Clinic
    • Place of Presentation
      Niseko, Hokkaido, Japan
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] TALEN-Mediated Disruption of Sphingolipid-Related Genes in a HeLa Cell Line2013

    • Author(s)
      Toshiyuki Yamaji and Kentaro Hanada
    • Organizer
      54th International Conference on the Bioscience of Lipids: Linking Transcription to Physiology in Lipidomics
    • Place of Presentation
      Bari, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] 人工ヌクレアーゼを利用したゲノム編集技術によるスフィンゴ脂質関連遺伝子破壊HeLa細胞変異株パネル作成の試み2013

    • Author(s)
      山地俊之、花田賢太郎
    • Organizer
      第8回スフィンゴテラピー研究会
    • Place of Presentation
      加賀
    • Related Report
      2013 Annual Research Report
  • [Presentation] 人工ヌクレアーゼTALENを用いた種々のスフィンゴ脂質関連遺伝子の変異株作製2013

    • Author(s)
      山地俊之、花田賢太郎
    • Organizer
      第32回日本糖質学会大会
    • Place of Presentation
      大阪
    • Related Report
      2013 Annual Research Report
  • [Presentation] 遺伝生化学的手法を用いたスフィンゴ糖脂質研究2013

    • Author(s)
      山地俊之、花田賢太郎
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細胞内セラミド輸送をつかさどるCERTの発見2013

    • Author(s)
      花田賢太郎
    • Organizer
      第6回セラミド研究会学術集会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Molecular architecture of CERT for inter-organelle transport of ceramide at the ER-Golgi membrane contact sites2013

    • Author(s)
      Kentaro Hanada
    • Organizer
      The 14th International Membrane Research Forum
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] 日本におけるワクチン国家検定制度2012

    • Author(s)
      花田賢太郎
    • Organizer
      バイオロジクスフォーラム第9回学術集会
    • Place of Presentation
      東京
    • Year and Date
      2012-02-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 哺乳動物細胞における膜脂質選別輸送2012

    • Author(s)
      花田賢太郎
    • Organizer
      理化学研究所・基幹研究所・細胞システムコロキウムシリーズIII 膜から探る生命秩序
    • Place of Presentation
      埼玉
    • Year and Date
      2012-01-25
    • Related Report
      2013 Final Research Report
  • [Presentation] A model of intracellular trafficking of the lipid ceramide2012

    • Author(s)
      Hanada K
    • Organizer
      2012 American Society of Photobiology
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Invited
  • [Presentation] Co-evolution of SM and CERT : Diverse lipids may require co-evolution of enzymes and trafficking system2012

    • Author(s)
      Hanada K
    • Organizer
      Riken Symposium, Evolution of Lipids
    • Place of Presentation
      Saitama, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Intracellular trafficking of ceramide by the ceramide trafficking protein CERT, Commemorative Symposium on the 20th Anniversary of the Mizutani Foundation for Glycoscience2012

    • Author(s)
      Hanada K
    • Organizer
      Glycoscience : Diversity and Integration
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Introductory remarks for the session of sphingolipid metabolism and Pathology2012

    • Author(s)
      Hanada K
    • Organizer
      Gordon Research Conference on Glycolipid and Sphingolipid Biology
    • Place of Presentation
      Rucca, Italy
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Structural basis for the preferential recognition of phosphatidylinositol 4-monophosphate by the pleckstrin homology domain of the ceramide trafficking protein CERT2012

    • Author(s)
      Hanada K, Sugiki T, Takeuchi K, Tokunaga, Y, Terasawa, H, Kumagai, K, Yamaji, T, Takahashi, H, Shimada, I
    • Organizer
      2012 ASBMB Special Symposium, Frontiers in Lipid Biology
    • Place of Presentation
      Banff, Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] 膜接触部位を介した宿主細胞セラミドのクラミジア菌寄生胞・封入体への輸送2012

    • Author(s)
      花田賢太郎
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] セラミド輸送タンパク質CERT の分子解析2012

    • Author(s)
      花田賢太郎
    • Organizer
      第5回セラミド研究会学術集会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] セラミド輸送タンパク質CERT とVAP との相互作用制御2012

    • Author(s)
      熊谷圭悟, 花田賢太郎
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural basis for the recognition of phosphatidylinositol 4-monophosphate by the pleckstrin homology domain of the ceramide trafficking protein CERT2012

    • Author(s)
      Kentaro Hanada, Toshihiko Sugiki, Koh Takeuchi, Yuji Tokunaga, Hiroaki Terasawa, Keigo Kumagai, Toshiyuki Yamaji, Hideo Takahashi, and Ichio Shimada
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] ジョアン・エンゲル : クラミジア菌の宿主細胞内増殖にセラミド輸送タンパク質CERTは重要である2012

    • Author(s)
      花田賢太郎
    • Organizer
      第54回日本脂質生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] Co-evolution of SM and CERT: Diverse lipids may require co-evolution of enzymes and trafficking system2012

    • Author(s)
      Kentaro Hanada
    • Organizer
      Riken Symposium, Evolution of Lipids
    • Place of Presentation
      埼玉県和光市
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Intracellular trafficking of ceramide by the ceramide trafficking protein CERT2012

    • Author(s)
      Kentaro Hanada
    • Organizer
      Commemorative Symposium on the 20th Anniversary of the Mizutani Foundation for Glycoscience, Glycoscience: Diversity and Integration
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Structural basis for the preferential recognition of phosphatidylinositol 4-monomphosphate by the pleckstrin homology domain of the ceramide trafficking protein CERT2012

    • Author(s)
      Kentaro Hanada
    • Organizer
      2012 ASBMB Special Symposium, Frontiers in Lipid Biology
    • Place of Presentation
      Banff, Canada
    • Related Report
      2012 Annual Research Report
  • [Presentation] クラミジア菌の宿主細胞内増殖にセラミド輸送タンパク質CERTは重要である2012

    • Author(s)
      花田賢太郎
    • Organizer
      第54回日本脂質生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] セラミド輸送タンパク質CERTの分子解析2012

    • Author(s)
      花田賢太郎
    • Organizer
      セラミド研究会第5回学術集会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Structural basis for the recognition of phosphatidylinositol 4-monomphosphate by the pleckstrin homology domain of the ceramide trafficking protein CERT2012

    • Author(s)
      Kentaro Hanada
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] Intracellular trafficking of ceramide by the ceramide transfer protein CERT2011

    • Author(s)
      Kentaro Hanada
    • Organizer
      The Annual Fall Scientific Conference of the Korean Society of Lipidology and Atherosclerosis
    • Place of Presentation
      韓国ソウル市(招待講演)
    • Year and Date
      2011-09-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] Intracellular trafficking of ceramide by the ceramide transfer protein CERT2011

    • Author(s)
      Hanada K
    • Organizer
      The Annual Fall Scientific Conference of the Korean Society of Lipidology and Atherosclerosis
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 抗がん性リモノイドはCERT による小胞体からのセラミドの引き抜きを阻害することによりスフィンゴミエリン合成を阻害する2011

    • Author(s)
      Francoise Hullin-Matsuda,冨重斉生,牧野麻美,石塚玲子,石井久美子,Elad Lavee Laviad,Anthony Futerman,Michel Lagarde, Hubert Vidal,花田賢太郎,小林俊秀
    • Organizer
      第6回スフィンゴテラピー研究会
    • Place of Presentation
      米子
    • Related Report
      2013 Final Research Report
  • [Presentation] Globotriaosylceramide (Gb3) is reduced by the expression of hydrophobic polypeptides including TMBIM family : Isolation of Shiga toxin-resistant genes2010

    • Author(s)
      Toshiyuki Yamaji
    • Organizer
      The 27^<th> Naito Conference on Membrane Dynamics and Lipid Biology [I]
    • Place of Presentation
      Sapporo, Hokkaido, Japan
    • Year and Date
      2010-07-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] CERT-dependent trafficking of ceramide2010

    • Author(s)
      Kentaro Hanada
    • Organizer
      The 27^<th> Naito Conference on Membrane Dynamics and Lipid Biology [I]
    • Place of Presentation
      Sapporo, Hokkaido, Japan
    • Year and Date
      2010-06-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Crystal structures of the CERT START domain in complex with lipid substrates and specific inhibitors provide insights into the molecular mechanisms of ceramide transfer2010

    • Author(s)
      Norio Kudo
    • Organizer
      The 27^<th> Naito Conference on Membrane Dynamics and Lipid Biology [I]
    • Place of Presentation
      Sapporo, Hokkaido, Japan
    • Year and Date
      2010-06-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] CERT-dependent trafficking of ceramide2010

    • Author(s)
      Hanada K
    • Organizer
      The 27th Naito Conference on Membrane Dynamics and Lipid Biology [I]
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Globotriaosylceramide (Gb3) is reduced by the expression of hydrophobic polypeptides including TMBIM family : Isolation of Shiga toxin-resistant genes2010

    • Author(s)
      Yamaji T, Nishikawa T, and Hanada K
    • Organizer
      The 27th Naito Conference on Membrane Dynamics and Lipid Biology [I]
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Crystal structures of the CERT START domain in complex with lipid substrates and specific inhibitors provide insights into the molecular mechanisms of ceramide transfer2010

    • Author(s)
      Kudo N, Kumagai K, Matsubara R, Kobayashi S, Wakatsuki S, Hanada, K, and Kato, R
    • Organizer
      The 27th Naito Conference on Membrane Dynamics and Lipid Biology [I]
    • Place of Presentation
      Sapporo
    • Related Report
      2013 Final Research Report
  • [Presentation] Structural biology revealed the serendipity of development of HPA-12, a potent inhibitor of intracellular trafficking of ceramide

    • Author(s)
      Hanada K
    • Organizer
      FASEB Science Research Conference : Lysophospholipid and other Related Mediators - From Bench to Clinic
    • Place of Presentation
      Niseko, Japan
    • Related Report
      2013 Final Research Report
    • Invited
  • [Remarks] 花田の研究テーマなど

    • URL

      http://www.nih.go.jp/niid/ja/from-biochem/3257-2013-02-25-06-28-53.html

    • Related Report
      2013 Final Research Report
  • [Remarks] TALEN技術を用いてのスフィンゴ脂質関連遺伝子の欠損したHeLa細胞変異株の樹立

    • URL

      http://www.niid.go.jp/niid/ja/basic-science/466-biochemistory/4431-biochem-2014-1.html

    • Related Report
      2013 Final Research Report
  • [Remarks] CERTのセリン315のリン酸化は,オルガネラ間のセラミド輸送に必要とされるCERT-VAP 間の相互作用を調節する

    • URL

      http://www.niid.go.jp/niid/ja/basic-science/466-biochemistory/4576-biochem-2014-2.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 花田の研究テーマなど

    • URL

      http://www.niid.go.jp/niid/ja/from-biochem/3257-2013-02-25-06-28-53.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] TALEN技術を用いてのスフィンゴ脂質関連遺伝子の欠損したHeLa細胞変異株の樹立

    • URL

      http://www.niid.go.jp/niid/ja/basic-science/466-biochemistory/4431-biochem-2014-1.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] CERTのセリン315のリン酸化はCERT-VAP間の相互作用を調節する

    • URL

      http://www.niid.go.jp/niid/ja/basic-science/466-biochemistory/4576-biochem-2014-2.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 花田の研究テーマなど

    • URL

      http://www.nih.go.jp/niid/ja/from-biochem/3257-2013-02-25-06-28-53.html

    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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