| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Research Abstract |
Lipid transfer proteins have been shown to mediate inter-organelle trafficking of various types of lipids in cells. However, mechanisms underlying the regulation of intracellular lipid trafficking remain poorly elucidated. In this research, we found i) phosphorylation at serine 315 of the CERT protein, which mediates transport of ceramide from the endoplasmic reticulum (ER) to the Golgi apparatus, strongly enhances the interaction of CERT with the ER-membrane protein VAP, and ii) this phosphorylation up-regulates ER-to-Golgi transport of ceramide. We previously showed that de-phosphorylation at another specific site of CERT up-regulates both activities for inter-membrane transfer of ceramide and Golgi-association of CERT. These results revealed that CERT-mediated ceramide trafficking is regulated by multiple protein kinase/phosphatase systems.
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