Comprehensive analysis of plasma membrane protein functions by immunomodulation

• Project/Area Number: 22380065
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Ibaraki University
• Principal Investigator: SUZUKI Yoshihito 茨城大学, 農学部, 教授 (90222067)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: イムノモジュレーション / 植物 / ブラシノステロイド / 細胞膜 / 膜タンパク質 / 一本鎖抗体 / 抗体 / サイトカイニン / 細胞膜タンパク質

Research Abstract

Immunomodulation study was applied to plasma membrane proteins. ER-targeted accumulation of an antibody against brassinosteroid receptor BRI1 resulted in transformants showing brassinosteroid-defective phenotype like dwarfism. Random introduction of antibodies, which were selected by the affinity to a plasma membrane protein fraction from a phage-display antibody library, gave some phenotypes to plants, which have yet to be related to the introduced antibodies.