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Long term culture and regulation of differentiation of germ cells from the testis in domestic species

Research Project

Project/Area Number 22380150
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionKyoto University

Principal Investigator

IMAI Hiroshi  京都大学, 大学院・農学研究科, 教授 (10303869)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywords発生工学 / 生殖幹細胞
Research Abstract

A long-term culture of male primitive germ cells from bovine testis has been examined. Bovine primitive germ cells can proliferate and survive in culture for over 2 months in the condition with serum-free components and on poly-L-lysine-coated dish. The resulting cell colonies have both pluripotent and stem cell potent characteristics. After culture of cells in the presence of growth factors, cells are differentiated into spermatogonia.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (39 results)

All 2013 2012 2011 2010 Other

All Journal Article (16 results) (of which Peer Reviewed: 15 results) Presentation (19 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Effects of extracellular matrices and lectin Dolichos biflorus agglutinin on cell adhesion and self-renewal of bovine gonocytes cultured in vitro2013

    • Author(s)
      Kim SM, Fujihara M, Sahare M, Minami N, Yamada M and Imai H
    • Journal Title

      Reproduction Fertility and Development

      Volume: In press Issue: 2 Pages: 268-268

    • DOI

      10.1071/rd12214

    • NAID

      120005367805

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein Kinase A Determines Timing of Early Differentiation through Epigenetic Regulation with G9a2012

    • Author(s)
      Yamamizu K, Fujihara M, Tachibana M, Katayama S, Takahashi A, Hara E, Imai H, Shinkai Y, and Yamashita JK
    • Journal Title

      Cell Stem Cell

      Volume: 10 Issue: 6 Pages: 759-770

    • DOI

      10.1016/j.stem.2012.02.022

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 哺乳動物におけるiPS細胞2012

    • Author(s)
      浅野良太
    • Journal Title

      日本胚移植研究会誌

      Volume: 34 Pages: 9-18

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system2011

    • Author(s)
      Tsukiyama T., Asano R., Kawaguchi T., Kim N., Yamada M., Minami N., Ohinata Y. and Imai H
    • Journal Title

      Genes to Cells

      Volume: 16 Issue: 7 Pages: 815-825

    • DOI

      10.1111/j.1365-2443.2011.01528.x

    • NAID

      120005593680

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization and in vitro culture of male germ cells from developing bovine testes2011

    • Author(s)
      Fujihara M, Kim SM, Minami N, Yamada M and Imai H
    • Journal Title

      Journal of Reproduction and Development

      Volume: 57 Pages: 355-364

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21289464

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of reprogramming factors associated with a pluripotent potential of porcine and bovine spermatic stem cells in culture2011

    • Author(s)
      Kim SM, Fujihara M, Goel S, Sahare M, Minami N, Yamada M and Imai H
    • Journal Title

      The Proceedings for 16th World Congress on In Vitro Fertilization

      Pages: 41-48

    • URL

      http://isivf2011.org/jp/

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] UCHL-1 protein expression specifically marks spermatogonia in wild bovid testis2011

    • Author(s)
      Goel S, Mahla RS, Suman SK, Reddy N and Imai H
    • Journal Title

      European Journal of Wildllife Research

      Volume: 57 Issue: 3 Pages: 663-667

    • DOI

      10.1007/s10344-010-0454-1

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Journal Article] Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos2011

    • Author(s)
      Miyamoto, K., Nagai, K., Kitamura, N., Nishikawa, T., Ikegami, H., Binh, N., Tsukamoto, S., Matsumoto, M., Tsukiyama, T., Minami, N., Yamada, M., Ariga, H., Miyake, M., Kawarasaki, T., Matsumoto, K., Imai, H.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 108 Issue: 17 Pages: 7040-7045

    • DOI

      10.1073/pnas.1013634108

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pluripotent stem cells from testis2011

    • Author(s)
      Goel S., Imai H.
    • Journal Title

      Embryonic Stem Cells-Differentiation and Pluripotent Alternatives(ed.Michael S.Kallos, InTech Publisher, Rijeka)

      Pages: 473-492

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of reprogramming factors associated with a pluripotent potential of porcine and bovine spermatic stem cells in culture2011

    • Author(s)
      Kim SM, Fujihara M, Goel S, Sahare M, Minami N, Yamada M, Imai H
    • Journal Title

      The Proceedings for 16th World Congress on In Vitro Fertilization

      Pages: 41-48

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of an oocyte factor required for development of porcine nuclear transfer embryos.2011

    • Author(s)
      Miyamoto K, Nagai K, Kitamura N, Nishikawa T, Ikegami H, Binh NT, Tsukamoto S, Matsumoto M, Tsukiyama T, Minami N, Yamada M, Ariga H, Miyake M, Kawarasaki T, Matsumoto K, Imai H.
    • Journal Title

      Proceedings of the National Academy of Science USA

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycycline-inducible factors and an EOS reporter system.2011

    • Author(s)
      Tsukiyama T, Asano R, Kawaguchi T, Kim N, Yamada M, Minami N, Ohinata Y, Imai H.
    • Journal Title

      Genes to Cells

      Volume: (印刷中)

    • NAID

      120005593680

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization and in vitro culture of male germ cells from developing bovine testis2011

    • Author(s)
      Fujihara M., Kim SM., Minami N, Yamada M., Imai H.
    • Journal Title

      Journal of Reproduction and Development

      Volume: (印刷中)

    • NAID

      10029050501

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] UCHL-1 protein expression specifically marks spermatogonia in wild bovid testis.2011

    • Author(s)
      Goel S, Mahla RS, Suman SK, Reddy N, Imai H.
    • Journal Title

      European Journal of Wild Animal Research

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spermatogonia-specific proteins expressed in prepubertal buffalo (Bubalus bubalis) testis and their utilization for isolation and in vitro cultivation of spermatogonia2010

    • Author(s)
      Goel S, Reddy N, Mandal S, Fujihara M, Kim SM and Imai H
    • Journal Title

      Theriogenology

      Volume: 74 Issue: 7 Pages: 1221-1232

    • DOI

      10.1016/j.theriogenology.2010.05.025

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spermatogonia specific proteins expressed in prepubertal buffalo (Bubalus bubalis) testis and their utilization for isolation and in vitro cultivation of spermatogonia.2010

    • Author(s)
      Goel S., Reddy N., Mandal S., Fujihara M., Kim SM., Imai H.
    • Journal Title

      Theriogenology

      Volume: 74 Pages: 1221-1232

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Long term self-renewal and proliferation of bovine male germ stem cells in vitro2013

    • Author(s)
      Sahare M, Kim SM, Otomo A, Komatsu K, Minami N, Yamada M and Imai H
    • Organizer
      CiRA International Symposium 2013 ~Raising the next generation of stem cell research
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Final Research Report
  • [Presentation] Long term self-renewal and proliferation of bovine male germ stem cells in vitro.2013

    • Author(s)
      今井 裕
    • Organizer
      CiRA International Symposium 2013 ~Raising the next generation of stem cell research~
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mitogen activated protein kinase (MAPK) signaling is indispensable for maintaning self renewal and proliferation of bovine male germ stem cells in culture2013

    • Author(s)
      今井 裕
    • Organizer
      The 2013 Society for Study of Reproduction (SSR) Annual Meeting
    • Place of Presentation
      Montreal, Canada
    • Related Report
      2012 Annual Research Report
  • [Presentation] Glycan epitopes involve in germ cell differentiation during early development of neonatal mice testis2012

    • Author(s)
      Kim SM, Fujihara M, Goel S, Minami N, Yamada M and Imai H
    • Organizer
      The 4th Congress of the Asia Pacific Initiative on Reproduction
    • Place of Presentation
      Osaka
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of GalNAc glycan residues associated adhesion and self-renewal in cultured bovine gonocytes2012

    • Author(s)
      Kim SM, Fujihara M, Sahare M, Minami N, Yamada M and Imai H
    • Organizer
      The 17th International Congress on Animal Reproduction (ICAR)
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] Determination of distinct germ cell commitment by the specific affinity of the lectin DBA during early development of gonocytes in neonatal mice testis2012

    • Author(s)
      KimSM, Fujihara M, Goel S, Minami N, Yamada M and Imai H
    • Organizer
      Symposium on Functional Genomics of Early Livestock Development
    • Place of Presentation
      Banff, Canada
    • Related Report
      2012 Final Research Report
  • [Presentation] Glycan epitopes involve in germ cell differentiation during early development of neonatal mice testis.2012

    • Author(s)
      今井 裕
    • Organizer
      The 4th Congress of the Asia Pacific Initiative on Reproduction
    • Place of Presentation
      Osaka, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] The role of GalNAc glycan residues associated adhesion and self-renewal in cultured bovine gonocytes.2012

    • Author(s)
      今井 裕
    • Organizer
      The 17th International Congress on Animal Reproduction (ICAR).
    • Place of Presentation
      Vancouver, Canada
    • Related Report
      2012 Annual Research Report
  • [Presentation] Determination of distinct germ cell commitment by the specific affinity of the lectin DBA during early development of gonocytes in neonatal mice testis.2012

    • Author(s)
      今井 裕
    • Organizer
      Symposium on Functional Genomics of Early Livestock Development
    • Place of Presentation
      Banff, Canada
    • Related Report
      2012 Annual Research Report
  • [Presentation] 家畜ならびに大型実験動物における多能性幹細胞株の樹立と応用2010

    • Author(s)
      築山智之・今井 裕
    • Organizer
      日本生化学会九州支部例会シンポジウム(招待講演)
    • Place of Presentation
      鹿児島市
    • Year and Date
      2010-05-23
    • Related Report
      2012 Final Research Report
  • [Presentation] 家畜ならびに大型実験動物における多能性幹細胞株の樹立と応用2010

    • Author(s)
      築山智之・今井裕
    • Organizer
      日本生化学会九州支部例会シンポジウム
    • Place of Presentation
      鹿児島大学(鹿児島)(招待講演)
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Comparative Changes on Stem Cell Potential of Bovine Male Germ Cells During Testicular Development from Neonatal to Adult2010

    • Author(s)
      Fujihara M, Kim SM, Minami N, Yamada M and Imai H
    • Organizer
      The 43rd Annual Meeting of the Study of the Society for Reproduction (SSR)
    • Place of Presentation
      Milwaukee USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Stem cells of the male germline: an aid to conservation of endangered species2010

    • Author(s)
      Goel S, Mandal S, Reddy N, Mahara R, Suman S, Raj TA, Fujihara M, Kim SM and Imai H
    • Organizer
      The 11th International Symposium on Spermatology (ISS)
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Lectin DBA specifically recognizes glycan cell surface epitope of gonocytes in developing mouse testis2010

    • Author(s)
      Kim SM, Fujihara M, Goel S, Minami N, Yamada M and Imai H
    • Organizer
      The 11th International Symposium on Spermatology (ISS)
    • Place of Presentation
      Okinawa, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Analysis of culture conditions for generation of germ-line competent induced pluripotent stem (iPS) cells in various mammalian species2010

    • Author(s)
      Tsukiyama T, Asano R, Minami N, Yamada M and Imai H
    • Organizer
      The 8th Annual Meeting of the International Society for Stem Cell Research
    • Place of Presentation
      San Francisco USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Comparative Changes on Stem Cell Potential of Bovine Male Germ Cells During Testicular Development from Neonatal to Adult2010

    • Author(s)
      Fujihara M, Kim SM, Minami N, Yamada M, Imai H.
    • Organizer
      The 43rd Annual Meeting of the Study of the Society for Reproduction (SSR)
    • Place of Presentation
      USA(Milwaukee)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Stem cells of the male germline : an aid to conservation of endangered species.2010

    • Author(s)
      Goel S, Mandal S, Reddy N, Mahara R, Suman S, Raj TA, Fujihara M, Kim SM, Imai H.
    • Organizer
      The 11th International Symposium on Spermatology(ISS)
    • Place of Presentation
      沖縄コンベンションセンター
    • Related Report
      2010 Annual Research Report
  • [Presentation] Lectin DBA specifically recognizes glycan cell surface epitope of gonocytes in developing mouse testis.2010

    • Author(s)
      Kim SM, Fujihara M, Goel S, Minami N, Yamada M, Imai H.
    • Organizer
      The 11th International Symposium on Spermatology(ISS)
    • Place of Presentation
      沖縄コンベンションセンター
    • Related Report
      2010 Annual Research Report
  • [Presentation] Analysis of culture conditions for generation of germ-line competent induced pluripotent stem(iPS)cells in various mammalian species.2010

    • Author(s)
      Tsukiyama T, Asano R, Minami N, Yamada M, Imai H.
    • Organizer
      The 8^<th> Annual Meeting of the International Society for Stem Cell Research
    • Place of Presentation
      USA(San Francisco)
    • Related Report
      2010 Annual Research Report
  • [Book] Pluripotent stem cells from testis."Embryonic Stem Cells - Differentiation and Pluripotent Alternatives"2011

    • Author(s)
      Goel S. and Imai H
    • Publisher
      ed. Michael S. Kallos、InTech Publisher、Rijeka
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.reprod.kais.kyoto-u.ac.jp/index.htm

    • Related Report
      2012 Final Research Report
  • [Remarks] 京都大学大学院農学研究科応用生物科学専攻生殖生物学分野

    • URL

      http://www.reprod.kais.kyoto-u.ac.jp/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.reprod.kais.kyoto-u.ac.jp/index.htm

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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