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Analysis of epiphytic bacteria-rice interaction using transcriptome technology and bioassay.

Research Project

Project/Area Number 22380183
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionNational Institute for Agro-Environmental Sciences

Principal Investigator

TSUSHIMA Seiya  独立行政法人農業環境技術研究所, 農業環境インベントリーセンター, センター長 (50354080)

Co-Investigator(Kenkyū-buntansha) MINAMI Eichi  独立行政法人農業生物資源研究所, 耐病性作物研究開発ユニット, 上席研究員 (70373256)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords葉面細菌 / 全細菌数計測 / 無菌イネ作出 / イネ遺伝子発現解析 / 細菌・イネ相互作用 / イネ遺伝子発現
Research Abstract

‘Rice with low epiphytic bacteria (RLE)’, which includes culturable and unculturable bacteria, was developed by sterilizing rice seed. The population of total epiphytic bacteria in RLE is significantly lower more than 1/1000 of that in wild rice. RLE is suggested to be useful for excluding genes’ expression by resident bacteria on plants, which we call it as ‘noise’ cuased by epiphytic bacterial, from genes’ expression of rice plant itself. Transcriptome analyses of RLE and wild rice were conducted. The results showed the increase in genes’ expression associated with DNA replication, DNA repair, protein synthesis and transport in RLE as compared to in wild rice. The results suggest that‘noises’ caused by bacteria inhabiting rice plant are included within the expressions of genes obtained by a microarray analysis of rice plants.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (10 results)

All 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (5 results) Remarks (2 results)

  • [Journal Article] Method for simply and rapidly enumerating total epiphytic bacteria in the washing solution of rice plants2011

    • Author(s)
      Rieko Niwa, Shigenobu Yoshida, Naruto Furuya, Kenichi Tsuchiya and Seiya Tsushima
    • Journal Title

      Can. J. Microibiology

      Volume: 57 Pages: 62-67

    • Related Report
      2012 Final Research Report
  • [Journal Article] Culturable bacterial communities on leaf sheaths and panicles of rice plants in Japan2011

    • Author(s)
      Hirosuke Shinohara, Shigenobu Yoshida, Junichiro Enya, Yukiko Watanabe, Takao Tsukiboshim, Hiromitsu Negishi, Seiya Tsushima
    • Journal Title

      Folia Microbiol

      Pages: 505-517

    • Related Report
      2012 Final Research Report
  • [Journal Article] Method for simple and rapid enumeration of total epiphytic bacteria in the washing solution of rice plants.2011

    • Author(s)
      R., Niwa, S., Yoshida, N., Furuya, K.Tsuchiya, S., Tsushima
    • Journal Title

      Canadian Journal of Microbiology

      Volume: 57 Pages: 62-67

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Dynamics of bacterial population on the surface of rice and wheat plant during the growing seasons2011

    • Author(s)
      Rieko Niwa, Shigenobu Yoshida, Naruto Furuya, Kenichi Tsuchiya, Seiya Tsushima
    • Organizer
      IUMS2011
    • Place of Presentation
      166
    • Related Report
      2012 Final Research Report
  • [Presentation] 植物表生細菌とイネの相互作用解明のための低葉面菌密度イネ作出法2011

    • Author(s)
      丹羽理恵子・南 栄一・大竹裕子・木村麻美子・對馬誠也
    • Organizer
      日植病報77
    • Place of Presentation
      258
    • Related Report
      2012 Final Research Report
  • [Presentation] イネゲノムマイクロアレイを用いた植物表生細菌とイネの相互作用の解明2011

    • Author(s)
      丹羽理恵子・南 栄一・大竹祐子・木村麻美子・對馬誠也
    • Organizer
      日本微生物生態学会
    • Related Report
      2012 Final Research Report
  • [Presentation] Dynamics of bacterial population on the surface of rice and wheat plant during the growing seasons2011

    • Author(s)
      Rieko Niwa, Shigenobu Yoshida, Naruto Furuya, Kenichi Tsuchiya, Seiya Tsushima
    • Organizer
      IUMS2011
    • Place of Presentation
      札幌市
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物表生細菌とイネの相互作用解明のための低葉面菌密度イネ作出法2011

    • Author(s)
      丹羽理恵子、南栄一、大竹祐子、木村麻美子、對馬誠也
    • Organizer
      日本植物病理学会
    • Place of Presentation
      東京農工大学
    • Related Report
      2010 Annual Research Report
  • [Remarks] 農業環境技術研究所 組織の概要の担当者業績紹介

    • URL

      http://www.niaes.affrc.go.jp/researcher/tsushima_s.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.niaes.affrc.go.jp/researcher/tsushima_s.html

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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