Analysis of plastid-targeting mechanism of glycoproteins involved in starch metabolism of rice
| Project/Area Number |
22380186
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Kimiko 新潟大学, 大学院・自然科学系, 准教授 (10281007)
HAYASHI Yasuko 新潟大学, 大学院・自然科学系, 准教授 (20228597)
HANASHIRO Isao 鹿児島大学, 農学部, 准教授 (30336325)
OHTSUBO Ken-ichi 新潟大学, 大学院・自然科学系, 教授 (80353960)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | アミラーゼ / ヌクレオチドピロホスファターゼ / スーパーオキシドジスムターゼ / ゴルジ体 / プラスチド / 葉緑体 / 糖タンパク質 / イネ / デンプン / 植物 / 糖鎖 / AmyI-1 / MSD1 |
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| Research Abstract |
(1) We found that rice AmyI-1, NPP1, NPP2, NPP6, and MSD1 are glycoproteins targeting to plastid via the secretory pathway, and we characterized N-glycome (oligosaccharide structures) of plastidial glycoprotein NPP1. (2) The plastid targeting signal regions (PT) of AmyI-1 and NPP1 were identified. Furthermore, it was suggested that there exists plastid envelope factor(s) that required for plastid targeting of glycoprotein. (3) Abnormal expression of alpha-amylase during seed development led to the poor formation of starch granule. In addition, NPP1 was found to be a negative regulator for starch accumulation in rice leaves.
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Report
(5 results)
Research Products
(50 results)
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[Journal Article] Crystal structure of α-amylase from Oryza sativa : Molecular insights into enzyme activity and thermostability2014
Author(s)
Akihito OCHIAI, Hiroshi SUGAI, Kazuki HARADA, Seiya TANAKA, Yohei ISHIYAMA, Kosuke ITO, Takaaki TANAKA, Toshio UCHIUMI, Masayuki TANIGUCHI, Toshiaki MITSUI
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: (In press)
Issue: 6
Pages: 989-997
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Crystal structure of α-amylase from Oryza sativa: Molecular insights into enzyme activity and thermostability2014
Author(s)
Akihito OCHIAI, Hiroshi SUGAI, Kazuki HARADA, Seiya TANAKA, Yohei ISHIYAMA, Kosuke ITO, Takaaki TANAKA, Toshio UCHIUMI, Masayuki TANIGUCHI, Toshiaki MITSUI
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Suppression of α-amylase genes improves quality of rice grain ripened under high temperature2012
Author(s)
Hakata, M., Kuroda, M., Miyashita, T., Yamaguchi, T., Kojima, M., Sakakibara, H., Mitsui, T. and Yamakawa, H.
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Journal Title
Plant Biotechnology Journal
Volume: 10
Issue: 9
Pages: 1110-1117
DOI
Related Report
Peer Reviewed
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