• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of plastid-targeting mechanism of glycoproteins involved in starch metabolism of rice

Research Project

Project/Area Number 22380186
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionNiigata University

Principal Investigator

MITSUI Toshiaki  新潟大学, 自然科学系, 教授 (70183960)

Co-Investigator(Kenkyū-buntansha) ITOH Kimiko  新潟大学, 大学院・自然科学系, 准教授 (10281007)
HAYASHI Yasuko  新潟大学, 大学院・自然科学系, 准教授 (20228597)
HANASHIRO Isao  鹿児島大学, 農学部, 准教授 (30336325)
OHTSUBO Ken-ichi  新潟大学, 大学院・自然科学系, 教授 (80353960)
Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Keywordsアミラーゼ / ヌクレオチドピロホスファターゼ / スーパーオキシドジスムターゼ / ゴルジ体 / プラスチド / 葉緑体 / 糖タンパク質 / イネ / デンプン / 植物 / 糖鎖 / AmyI-1 / MSD1
Research Abstract

(1) We found that rice AmyI-1, NPP1, NPP2, NPP6, and MSD1 are glycoproteins targeting to plastid via the secretory pathway, and we characterized N-glycome (oligosaccharide structures) of plastidial glycoprotein NPP1. (2) The plastid targeting signal regions (PT) of AmyI-1 and NPP1 were identified. Furthermore, it was suggested that there exists plastid envelope factor(s) that required for plastid targeting of glycoprotein. (3) Abnormal expression of alpha-amylase during seed development led to the poor formation of starch granule. In addition, NPP1 was found to be a negative regulator for starch accumulation in rice leaves.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report

Research Products

(50 results)

All 2014 2013 2012 2011 2010

All Journal Article (17 results) (of which Peer Reviewed: 15 results,  Open Access: 1 results) Presentation (24 results) (of which Invited: 2 results) Book (5 results) Patent(Industrial Property Rights) (4 results)

  • [Journal Article] Nucleotide pyrophosphatase/phosphodiesterase 1 exerts a negative effect on starch accumulation and growth in rice seedlings under high temperature and CO2 concentration conditions2014

    • Author(s)
      Kentaro KANEKO, Takuya INOMATA, Takahiro MASUI, Tsutomu KOSHU, Yukiho UMEZAWA, Javier POZUETA-ROMERO, Toshiaki MITSUI
    • Journal Title

      Plant and Cell Physiology

      Volume: 55(2) Pages: 320-332

    • DOI

      10.1093/pcp/pct139

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of α-amylase from Oryza sativa : Molecular insights into enzyme activity and thermostability2014

    • Author(s)
      Akihito OCHIAI, Hiroshi SUGAI, Kazuki HARADA, Seiya TANAKA, Yohei ISHIYAMA, Kosuke ITO, Takaaki TANAKA, Toshio UCHIUMI, Masayuki TANIGUCHI, Toshiaki MITSUI
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: (In press) Pages: 989-997

    • DOI

      10.1080/09168451.2014.917261

    • Related Report
      2013 Final Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Crystal structure of α-amylase from Oryza sativa: Molecular insights into enzyme activity and thermostability2014

    • Author(s)
      Akihito OCHIAI, Hiroshi SUGAI, Kazuki HARADA, Seiya TANAKA, Yohei ISHIYAMA, Kosuke ITO, Takaaki TANAKA, Toshio UCHIUMI, Masayuki TANIGUCHI, Toshiaki MITSUI
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 印刷中

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of Opaque and Translucent Parts of High Temperature Stressed Grains of Rice2013

    • Author(s)
      Kazumi TSUTSUI, Kentaro KANEKO, Isao HANASHIRO, Katsuyoshi NISHINARI, Toshiaki MITSUI
    • Journal Title

      J. Appl. Glycosci.

      Volume: 60 Issue: 1 Pages: 61-67

    • DOI

      10.5458/jag.jag.jag-2012_014

      10.5458/jag.jag.JAG-2012_014

    • NAID

      130004481019

    • ISSN
      1344-7882, 1880-7291
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高温でコメに乳白粒が発生する原因を遺伝子レベルで解明-高温登熟耐性品種の開発に期待-2013

    • Author(s)
      山川 博幹
    • Journal Title

      JATAFFジャーナル

      Volume: 1(4) Pages: 24-27

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Suppression of α-amylase genes improves quality of rice grain ripened under high temperature2012

    • Author(s)
      Hakata, M., Kuroda, M., Miyashita, T., Yamaguchi, T., Kojima, M., Sakakibara, H., Mitsui, T. and Yamakawa, H.
    • Journal Title

      Plant Biotechnology Journal

      Volume: 10 Pages: 1110-1117

    • DOI

      10.1111/j.1467-7652.2012.00741.x

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A null mutation of ROS1a for DNA demethylation in rice is not transmittable to progeny2012

    • Author(s)
      Akemi ONO, Katsushi YAMAGUCHI, Sachiko FUKADA-TANAKA, Rie TERADA, Toshiaki MITSUI, Shigeru IIDA
    • Journal Title

      The Plant Journal

      Volume: 71(4) Pages: 564-574

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lack of starch synthase IIIa and high expression of granule-bound starch synthase I synergistically increase the apparent amylose content in rice endosperm2012

    • Author(s)
      Naoko CROFTS, Katsumi ABE, Satomi AIHARA, Rumiko ITOH, Yasunori NAKAMURA, Kimiko ITOH, Naoko FUJITA
    • Journal Title

      Plant Science

      Volume: 62(9) Pages: 193-194

    • DOI

      10.1016/j.plantsci.2012.05.006

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rice kinesin O12 is identical to kinesin OsKCH12012

    • Author(s)
      Nozomi Umezu, Nobuhisa Umeki,Toshiaki Mitsui, Kazunori Kondo, Shinsaku Maruta
    • Journal Title

      The Journal of Biochemistry

      Volume: 152(2) Pages: 207-208

    • DOI

      10.1093/jb/mvs076

    • NAID

      10031166130

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of Endomembrane-type Superoxide Dismutase in High Temperature Stress Tolerance during Grain Filling of Rice2012

    • Author(s)
      Toshiaki Mitsui
    • Journal Title

      Proceedings of the 3^<rd> International Symposium "Frontiers in Agriculture Proteome Reseach"

      Pages: 56-61

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of Microbodies in Chlamydomonas reinhardtii2011

    • Author(s)
      Yasuko HAYASHI, Akiko SHINOZAKI
    • Journal Title

      Journal of Plant Reserch

      Volume: 125(4) Pages: 579-586

    • DOI

      10.1007/s10265-011-0469-z

    • NAID

      10030804091

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical characterization of the novel rice kinesin K23 and its kinetic study using fluorescence resonance energy transfer between an intrinsic tryptophan residue and a fluorescent ATP analogue2011

    • Author(s)
      Nozomi UMEZU, Nobue HANZAWA, Masafumi D. YAMADA, Kazunori KONDO, Toshiaki MITSUI, Shinsaku MARUTA
    • Journal Title

      The Journal of Biochemistry

      Volume: 149 Pages: 539-550

    • DOI

      10.1093/jb/mvr012

    • NAID

      10030585182

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential localizations and functions of rice nucleotide pyrophosphatase/phosphodiesterase isozymes 1 and 32011

    • Author(s)
      Kentaro KANEKO, Chie YAMADA, Ai YANAGIDA, Tsutomu KOSHU, Ukiho UMEZAWA, Kimiko ITOH, Hidetaka HORI, Toshiaki MITSUI
    • Journal Title

      Plant Biotechnology

      Volume: 28(1) Issue: 1 Pages: 69-76

    • DOI

      10.5511/plantbiotechnology.10.1228a

    • NAID

      10028258633

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential localizations and functions of rice nucleotide pyrophosphate-ase/phosphodiesterase isozymes 1 and 32011

    • Author(s)
      Kentaro Kaneko
    • Journal Title

      Plant Biotechnology

      Volume: 28 Pages: 69-76

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystallographic analysis reveals a unique conformation of the ADP-bound novel rice kinesin K162010

    • Author(s)
      Keiko TANAKA, Nobuhisa UMEKI,Toshiaki MITSUI, Zui FUJIMOTO, Shinsaku MARUTA
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 401(2) Pages: 251-256

    • DOI

      10.1016/j.bbrc.2010.09.043

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallographic analysis reveals a unique conformation of the ADP-bound novel rice kinesin K162010

    • Author(s)
      Keiko Tanaka
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 401 Pages: 251-256

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis and Degradation of Starch2010

    • Author(s)
      Toshiaki Mitsui
    • Journal Title

      Bulletin of the Faculty of Agriculture Niigata University

      Volume: 62 Pages: 49-73

    • NAID

      120006741753

    • Related Report
      2010 Annual Research Report
  • [Presentation] フェムト秒レーザーを用いた光依存的オルガネラ間接着力評価2014

    • Author(s)
      及川和聡、真野昌二、林誠、山田健志、近藤真紀、東正一、渡辺正勝、三ツ井敏明、飯野敬矩、重政彰徳、細川陽一郎、西村幹夫
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Golgi/plastid-type superoxide dismutase involves in high temperature stress tolerance during grain filling of rice2014

    • Author(s)
      Maiko Sasaki, Takeshi Shiraya, Tatsuya Maruyama, Toshiaki Mitsui
    • Organizer
      第55回日本植物生理学会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 米型経口コレラワクチンMarker Free MucoRice-CTBのイネゲノム導入部位と導入配列について2014

    • Author(s)
      目島未央、鹿島光司、黒田昌治、竹内夏実、黒河志保、福山賀子、清野宏、三ツ井敏明、幸義和
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] イネα-アミラーゼの機能発現制御と米品質2013

    • Author(s)
      三ツ井敏明
    • Organizer
      植物化学調節学会第48回大会
    • Place of Presentation
      新潟大学
    • Year and Date
      2013-11-01
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 高温被害米の物理化学的特性の解明2013

    • Author(s)
      筒井和美、金古堅太郎、花城 勲、西成勝好、三ツ井敏明
    • Organizer
      日本調理科学会 東海・北陸支部 第10回研究発表会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 高温被害米の澱粉の糊化特性2013

    • Author(s)
      筒井和美、斎藤由佳、三ツ井敏明、西成勝好
    • Organizer
      第60回記念大会 日本食品科学工学会
    • Place of Presentation
      実践女子大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Mutation of nucleotide pyrophosphatase/phosphodiesterase 1 stimulates accumulation of starch in rice seedlings under high CO2 concentration2013

    • Author(s)
      Takahiro MASUI, Takuya INOMATA, Maiko SASAKI, Kentaro KANEKO, Toshiaki MITSUI
    • Organizer
      12th HUPO World Congress 2013
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] Starch glycomic and proteomic aspects of grain chalkness2013

    • Author(s)
      Kentaro KANEKO, Maiko SASAKI, Tatsuya MARUYAMA, Takeshi SHIRAYA, Toshiaki MITSUI
    • Organizer
      12th HUPO World Congress 2013
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] Comprehensive identification of OsSUMO binding proteins in rice exposed to high temperature stress condition2013

    • Author(s)
      Takuya KUSHIOKA, Hiroshi ATAKA, Attia KOTB,Kentaro KANEKO, Toshiaki MITSUI, Kimiko ITOH
    • Organizer
      12th HUPO World Congress 2013
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] 乳白米粒の澱粉・プロテオーム解析2013

    • Author(s)
      佐々木麻衣子、金古堅太郎、丸山達也、白矢武士、澤田隆行、三ツ井敏明
    • Organizer
      日本応用糖質科学会平成25年度大会
    • Place of Presentation
      鹿児島大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] デンプンのヨウ素吸収曲線の分析値と糊化特性値を用いた米のアミロース含量、アミロペクチン重合度、レジスタントスターチ含量の推定式の開発2013

    • Author(s)
      中村澄子、丸山恭弘、桂 順二、佐藤 光、大坪研一
    • Organizer
      日本水稲品質・食味研究会第5回講演会
    • Place of Presentation
      香川大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Characteristics of various rice grains stressed at high temperature2013

    • Author(s)
      Kazumi TSUTSUI, C. Nishikawa, Toshiaki MITSUI, Kentaro KANEKO, Kazutosi HAYASE and Katsuyoshi NISHINARI
    • Organizer
      第23回日本MRS年次大会
    • Place of Presentation
      yokohama
    • Related Report
      2013 Annual Research Report
  • [Presentation] Diverse localization of rice membrane protein TMN12013

    • Author(s)
      Ryuichi Ishiyama
    • Organizer
      The 54th Annual Meeting of the Japanese Society of Plant Physiologists
    • Place of Presentation
      岡山大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 新規アミロライスの胚乳および分離澱粉の構造解析とレオロジー特性2012

    • Author(s)
      阿久澤さゆり
    • Organizer
      日本食品科学工学会第59回大会
    • Place of Presentation
      藤女子大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高アミロースデンプン/アガロースゲルの物性の解析2012

    • Author(s)
      白鳥龍一
    • Organizer
      日本応用糖質学会平成24年度大会
    • Place of Presentation
      東京農工大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] イネゴルジ体のプロテオーム解析2012

    • Author(s)
      石山隆一
    • Organizer
      平成24年度 日本プロテオーム学会年会
    • Place of Presentation
      日本科学未来館
    • Related Report
      2012 Annual Research Report
  • [Presentation] α-アミラーゼの抑制はイネの高温登熟障害を軽減する2012

    • Author(s)
      羽方誠
    • Organizer
      日本応用糖質学会平成24年度大会
    • Place of Presentation
      東京農工大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 稲澱粉代謝関連酵素の細胞分子生物学と高温耐性稲の開発戦略2011

    • Author(s)
      三ツ井敏明
    • Organizer
      日本水稲品質・食味研究会
    • Place of Presentation
      新潟大学
    • Year and Date
      2011-11-23
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] Involvement of endomembrane-type superoxide dismutase in high temperature stress tolerance during grain filling of rice2011

    • Author(s)
      Toshiaki Mitsui
    • Organizer
      農学プロテオーム学会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2011-11-09
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] Golgi-to-Plastid traffic in higher plant cells2011

    • Author(s)
      Toshiaki Mitsui
    • Organizer
      日本生化学会
    • Place of Presentation
      京都国際会館
    • Year and Date
      2011-09-24
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] イネプラスチド局在型ヌクレオチドピロホスファターゼ/ホスホジ エステラーゼのN-グライコーム解析 : 機能糖タンパク質がゴルジ装置の後期区画からプラスチドに輸送される2011

    • Author(s)
      三ツ井敏明
    • Organizer
      日本プロテオーム学会
    • Place of Presentation
      朱鷺メッセ(新潟)
    • Year and Date
      2011-07-29
    • Related Report
      2013 Final Research Report
  • [Presentation] イネプラスチド局在型ヌクレオチドピロホスファターゼ/ホスホジエステラーゼのN-グライコーム解析:機能糖タンパク質がゴルジ装置の後期区画からプラスチドに輸送される2011

    • Author(s)
      三ツ井敏明
    • Organizer
      日本プロテオーム学会
    • Place of Presentation
      朱鷺メッセ(新潟)
    • Year and Date
      2011-07-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 高温ストレスに強いイネを 開発する2010

    • Author(s)
      三ツ井敏明
    • Organizer
      新農業展開ゲノムプロジェク ト・富山シンポジウム2010「ここまでで きた!お米の研究最前線」
    • Place of Presentation
      富山県民会鎮
    • Year and Date
      2010-12-10
    • Related Report
      2013 Final Research Report
  • [Presentation] 高温ストレスに強いイネを開発する2010

    • Author(s)
      三ツ井敏明
    • Organizer
      新農業展開ゲノムプロジェクト・富山シンポジウム2010「ここまでできた!お米の研究最前線」
    • Place of Presentation
      富山県民会鎮
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Book] プロテオミクス辞典2013

    • Author(s)
      三ツ井 敏明, 金古 堅太郎, 他
    • Total Pages
      127
    • Publisher
      講談社
    • Related Report
      2013 Final Research Report
  • [Book] Plant Proteomics Methods and Protocols 2nd edition2013

    • Author(s)
      Kentaro KANEKO, Takeshi SHIRAYA, Toshiaki MITSUIKentaro KANEKO, Takeshi SHIRAYA, Toshiaki MITSUI, Shin-ichiro NISHIMURA
    • Total Pages
      789
    • Publisher
      Humana press
    • Related Report
      2013 Final Research Report
  • [Book] プロテオミクス辞典2013

    • Author(s)
      三ツ井敏明・金古堅太郎 他
    • Total Pages
      127
    • Publisher
      講談社
    • Related Report
      2013 Annual Research Report
  • [Book] Plant Proteomics Methods and Protocols 2nd edition2013

    • Author(s)
      Kentaro KANEKO, Takeshi SHIRAYA, Toshiaki MITSUI, Shin-ichiro NISHIMURA
    • Total Pages
      786
    • Publisher
      Humana press
    • Related Report
      2013 Annual Research Report
  • [Book] Research Communications In Biochemistry And Cell & Molecular Biology Including Oxygen Biology, and Generation and Regeneration(Plant Organelle Proteomics : Approach and Applications)Vol.132010

    • Author(s)
      Setsuko Komatsu
    • Publisher
      PJD Publications Limited Research Communication Journals Biomedical Publisher to the World
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] α-アミラーゼ遺伝子の発現を抑制するRNA又はDNA, 及びそれらの利用2012

    • Inventor(s)
      三ツ井敏明, 他
    • Industrial Property Rights Holder
      新潟大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-03-30
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] α-アミラーゼ遺伝子の発現を抑制するRNA又はDNA、及びそれらの利用2012

    • Inventor(s)
      三ツ井敏明
    • Industrial Property Rights Holder
      新潟大学
    • Industrial Property Number
      2012-080709
    • Filing Date
      2012-03-30
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 高温登熟性イネ2011

    • Inventor(s)
      三ツ井敏明
    • Industrial Property Rights Holder
      新潟大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2011-11-10
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 高温登熟性イネ2011

    • Inventor(s)
      三ツ井敏明
    • Industrial Property Rights Holder
      新潟大学
    • Industrial Property Number
      2011-246579
    • Filing Date
      2011-11-10
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2010-08-22   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi