| Project/Area Number |
22390222
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
MORI Norio 浜松医科大学, 医学部, 教授 (00174376)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Katsuaki 浜松医科大学, 医学部, 准教授 (00285040)
TSUCHIYA Kenji 浜松医科大学, 子どものこころの発達研究センター, 准教授 (20362189)
MINOSHIMA Shinsei 浜松医科大学, メディカルフォトニクス研究センター, 教授 (90181966)
MATSUZAKI Hideo 福井大学, 子どものこころの発達研究センター, 教授 (00334970)
NAKAMURA Kazuhiko 弘前大学, 医学系研究科, 教授 (80263911)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
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| Keywords | 児童・思春期精神医学 / 自閉症 / バイオマーカー / サイトカイン / コホート研究 / Copy number variation |
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| Research Abstract |
The aim of the study was to explore biological markers in peripheral plasmasamples from non-medicated subjects with high-functioning ASD.(Experiment 1) A multiplex assay for cytokines and chemokines was applied to plasma samples frommale subjects with high-functioning ASD (n = 28) and matched controls (n = 28). Among a total of 48 analytes examined, theplasma concentrations of IL-1β, IL-1RA, IL-5, IL-8, IL-12(p70), IL-13, IL-17 and GRO-αwere significantly higher in subjects withASD compared with the corresponding values of matched controls after correction for multiple comparisons.(Experiment 2) Plasma levels of 25 amino acids in male children (n= 23) with ASDand matchedcontrols (n= 22) were determined using high-performance liquid chromatography. Compared with controls, subjects with ASD showed higher levels of plasmaglutamate and lower levels of plasma glutamine. Using discriminantanalysis with logistic regression, the two values of plasma glutamate and glutamine were shown to differentiate theASDgroup from the control group; the rate of correct classification was 91%.Theseresults suggest that abnormal immune responses as assessed by multiplex analysis and plasma glutamate and glutamine levelsmay serve as the biological markers for ASD.
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