The identification of drug resistance in ovarian cancer : new insights form metabolomics and autophagy

• Project/Area Number: 22390308
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Yamagata University
• Principal Investigator: KURACHI HIROHISA 山形大学, 医学部, 教授 (40153366)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Toshifumi 山形大学, 医学部, 講師 (20302292) ; OHTA Tsuyoshi 山形大学, 医学部, 助教 (50375341) ; TAKATA Keiko 山形大学, 医学部, 助教 (平成22 年度) (70375343) ; SUTOH Takeshi 山形大学, 医学部, 助教 (70466605) ; MABUCHI Seiji 大阪大学, 医学部, 助教 (00452441) ; SAWADA Kenjiro 大阪大学, 医学部, 講師 (00452392)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2013: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 卵巣がん / 薬剤耐性化 / Rho/ROCK経路 / EGF受容体 / Trabectedin / ハニカム膜 / mTOR / メタボローム解析 / 卵巣癌 / 卵巣明細胞腺癌 / microRNA / 細胞増殖 / 細胞接着 / 卵巣癌幹細胞 / オートファジー / メタボロミクス / 薬剤耐性化機構 / トラベクテジン / AKT-mTORシグナル / 薬剤耐性

Research Abstract

We identified that the anti-tumor activity of trabectedin was greater than any other existing anti-cancer agents, indicating that trabectedin is a promising agent for ovarian clear cell carcinoma (CCC). We also identified that combination treatment with trabectedin and irinotecan or topotecan displays the greatest cytotoxic effect against ovarian CCC. We believe that our studies provide the rationale for future clinical trials of trabectedin-based combination chemotehrapies in patients with ovarian CCC. We are currently investigating the role of autophagy in the sensitivity of CCC cells to trabectedin-based combination chemotherapies. We also identified that high levels of WT1 expression correlated with aggressive clinical features in ovarian cancer. The median disease-free survival time in patients with high WT1 expression levels was significantly shorter (p=0.038) than that in patients with low WT1 expression.

Research Products

• [Journal Article] Redistribution of resistance and sensitivity to platinum during the observation period following treatment of epithelial ovarian cancer (2014)
  • Author(s): Yokoyama Y, Kurachi H
  • Journal Title: Mol clin oncol Volume: 2 Issue: 2 Pages: 212-218
  • DOI: 10.3892/mco.2013.223
  • Peer Reviewed
• [Journal Article] miR-92a inhibits peritoneal dissemination of ovarian cancer cells by inhibiting integrin α5 expression (2013)
  • Author(s): Ohyagi-Hara C, Sawada K, Mabuchi S, Takahashi T, Kurachi H
  • Journal Title: Am J Pathol Volume: 182(5) Issue: 5 Pages: 1876-1889
  • DOI: 10.1016/j.ajpath.2013.01.039
  • Peer Reviewed
• [Journal Article] Ovarian tumors with functioning stroma : a clinicopathologic study with special reference to serum estrogen level, stromal morphology, and aromatase expression (2013)
  • Author(s): Kato N, Hayasaka T, Takeda J, Osakabe M, Kurachi H
  • Journal Title: Int J Gynecol Pathol Volume: 32(6) Issue: 6 Pages: 556-561
  • DOI: 10.1097/pgp.0b013e31827c6362
  • Peer Reviewed
• [Journal Article] Non-sex cord-stromal ovarian tumors frequently produce and secrete estrogen in postmenopausal women : impact on bone metabolism and abnormal endometrial histology (2013)
  • Author(s): Matsumura S, Ohta T, Takahashi T, T Kurachi H
  • Journal Title: J Clin Endocrinol Metab Volume: 98(7) Issue: 7 Pages: 2775-2782
  • DOI: 10.1210/jc.2013-1267
  • Peer Reviewed
• [Journal Article] Investigation of the clinicopathological features of fallopian tube malignancy (2013)
  • Author(s): Yokoyama Y, Kurachi H
  • Journal Title: Oncol Rep Volume: 30 Issue: 1 Pages: 79-84
  • DOI: 10.3892/or.2013.2439
  • Peer Reviewed
• [Journal Article] Non-sex cord-stromal ovarian tumors frequently produce and secrete estrogen in postmenopausal women:impact on bone metabolism and abnormal endometrial histology. (2013)
  • Author(s): Matsumura S, Ohta T, Takahashi T, T Kurachi H. （他2名、6番目）
  • Journal Title: J Clin Endocrinol Metab Volume: 98 Pages: 2775-2782
  • Peer Reviewed
• [Journal Article] miR-92a inhibits peritoneal dissemination of ovarian cancer cells by inhibiting integrin α5 expression. (2013)
  • Author(s): Kato N, Hayasaka T, Takeda J, Osakabe M, Kurachi H. （他3名、5番目）
  • Journal Title: Int J Gynecol Pathol Volume: 32 Pages: 556-561
  • Peer Reviewed
• [Journal Article] miR-92a inhibits peritoneal dissemination of ovarian cancer cells by inhibiting integrin α5 expression. (2013)
  • Author(s): Ohyagi-Hara C, Sawada K, Mabuchi S, Takahashi T, Kurachi H.（他12名、15番目）
  • Journal Title: Am J Pathol Volume: 182 Pages: 1876-1889
  • Peer Reviewed
• [Journal Article] MiR-92a inhibits peritoneal dissemination of ovarian cancer cells by inhibiting integrin α5 expression. (2013)
  • Author(s): Ohyagi-Hara C, Sawada K, Kamiura S, Tomita Y, Isobe A, Hashimoto K, Kinose Y, Mabuchi S, Hisamatsu T, Takahashi T, Kumasawa K, Nagata S, Morishige K-I, Lengyel E, Kurachi H, Kimura T.
  • Journal Title: Am J Pathol Volume: in press
• [Journal Article] Inhibition of the Rho/ROCK pathway enhances the efficacy of cisplatin through the blockage of hypoxia-inducible factor-1α in human ovarian cancer cells (2012)
  • Author(s): Ohta T, Takahashi T, Kurachi H
  • Journal Title: Cacer Biol Ther Volume: 13(7) Issue: 1 Pages: 1-9
  • DOI: 10.4161/cbt.13.1.18440
  • Peer Reviewed
• [Journal Article] Gefitinib (ZD1839) increases the efficacy of cisplatin in ovarian cancer cells (2012)
  • Author(s): Ohta T, Takahashi T, Kurachi H
  • Journal Title: Cancer Biol Ther Volume: 13(6) Issue: 6 Pages: 408-416
  • DOI: 10.4161/cbt.19292
  • Peer Reviewed
• [Journal Article] Histological grading of ovarian clear cell adenocarcinoma : proposal for a simple and reproducible grouping system based on tumor growth architecture (2012)
  • Author(s): Yamamoto S, Kurachi H
  • Journal Title: Int J Gynecol Pathol Volume: 31(2) Issue: 2 Pages: 116-124
  • DOI: 10.1097/pgp.0b013e3182285c90
  • Peer Reviewed
• [Journal Article] 婦人科腫瘍との鑑別が困難であったGISTの2例. (2012)
  • Author(s): 松村創平,太田 剛,水谷雅臣,蜂谷 修,木村 理,菅井幸雄,高橋一広,倉智博久.
  • Journal Title: 臨床婦人科産科 Volume: 66（9） Pages: 799-802
  • Peer Reviewed
• [Journal Article] Phase II clinical study of the combination chemotherapy regimen of irinotecan plus oral etoposide for the treatment of recurrent ovarian cancer. (Tohoku Gynecologic Cancer Unit 101 Group Study) (2011)
  • Author(s): Shoji T, Kurachi H
  • Journal Title: Int J Gynecol Cancer Volume: 1(1) Issue: 1 Pages: 44-50
  • DOI: 10.1097/igc.0b013e3181ffbe9f
  • Peer Reviewed
• [Journal Article] Validation of the histologic grading for ovarian clear cell adenocarcinoma : a retrospective multi-institutional study by the Japan clear cell carcinoma study group (2011)
  • Author(s): Yamamoto S, Kurachi H
  • Journal Title: Int J Gynecol Pathol Volume: 30(2) Issue: 0 Pages: 129-138
  • DOI: 10.1097/pgp.0b013e3181f71264
  • Peer Reviewed
• [Journal Article] Identifying prognostic factors in Japanese women with pseudomyxoma peritonei : a retrospective clinico-pathological study of the Tohoku Gynecologic Cancer Unit (2011)
  • Author(s): Kojimahara T, Kurachi H
  • Journal Title: Tohoku J Exp Med Volume: 223(2) Pages: 91-96
  • NAID: 10027806806
  • URL: https://www.jstage.jst.go.jp/article/tjem/223/2/223_2_91/_pdf
  • Peer Reviewed
• [Journal Article] Clear cell adenocarcinoma with a component of poorly differentiated histology : a poor prognostic subgroup of ovarian clear cell adenocarcinoma (2011)
  • Author(s): Yamamoto S, Kurachi H
  • Journal Title: Int J Gynecol Pathol Volume: 30(5) Issue: 5 Pages: 431-441
  • DOI: 10.1097/pgp.0b013e3182165eba
  • Peer Reviewed
• [Journal Article] The activity of trabectedin as a single agent or in combination with everolimus for clear cell carcinoma of the ovary (2011)
  • Author(s): Mabuchi S, Sawada K, Takahashi T, Kurachi H
  • Journal Title: Clin Cancer Res Volume: 17(13) Issue: 13 Pages: 4462-4473
  • DOI: 10.1158/1078-0432.ccr-10-2987
  • Peer Reviewed
• [Journal Article] Identifying Prognostic Factors in Japanese Women with Pseudomyxoma Peritonei: A Retrospective Clinico-Pathological Study of the Tohoku Gynecologic Cancer Unit (2011)
  • Author(s): Kojimahara T, Nakahara K, Shoji T, Sugiyama T, Takano T, Yaegashi N, Yokoyama Y Mizunuma H, Tase R, Satou H, Tanaka T, Motoyama T, Kurachi H
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 223 Issue: 2 Pages: 91-96
  • DOI: 10.1620/tjem.223.91
  • NAID: 130004459878
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed
• [Journal Article] Phase II clinical study of the combination chemotherapy regimen of irinotecan plus oral etoposide for the treatment of recurrent ovarian cancer. (Tohoku Gynecologic Cancer Unit 101 Group Study) (2011)
  • Author(s): Shoji T, Takatori E, Omi H, Kumagai S, Yoshizaki A, Yokoyama Y, Mizunuma H, Fujimoto T, Tkano T, Yaegashi N, Tase T, Nakahara K, Kurachi H, Nishiyama H, Sugiyama T
  • Journal Title: Int J Gynecol Cancer Volume: 21 Pages: 44-50
  • Peer Reviewed
• [Journal Article] Estrogen induces neurite outgrowth via Rho family GTPases in neuroblastoma cells (2011)
  • Author(s): Takahashi K, Piao S, Yamatani H, Du B, Yin L, Ohta T, Kawagoe J, Takata K, Tsutsumi S, Kurachi H
  • Journal Title: Mol Cell Neurosci Volume: 48 Issue: 3 Pages: 217-224
  • DOI: 10.1016/j.mcn.2011.08.002
  • Peer Reviewed
• [Journal Article] Validation of the histologic grading for ovarian clear cell adenocarcinoma : a retrospective multi-institutional study by the Japan clear cell carcinoma study group (2011)
  • Author(s): Yamamoto S, Kasajima A, Takano M, Yaegashi N, Fujiwara H, Kuzuya K, Kigawa J, Tsuda H, Kurachi H, Kikuchi Y, Sugiyama T, Tsuda H, Moriya T
  • Journal Title: Int J Gynecol Pathol Volume: 30 Pages: 129-138
  • Peer Reviewed
• [Journal Article] Clear cell adenocarcinoma with a component of poorly differentiated histology : a poor prognostic subgroup of ovarian clear cell adenocarcinoma (2011)
  • Author(s): Yamamoto S, Tsuda H, Shimazaki H, Takano M, Yoshikawa T, Kuzuya K, Tsuda H, Kurachi H, Kigawa J, Kikuchi Y, Sugiyama T
  • Journal Title: Int J Gynecol Path Volume: 30 Pages: 431-441
  • Peer Reviewed
• [Journal Article] Histological grading of ovarian clear cell adenocarcinoma: proposal for a simple and reproducible grouping system based on tumor growth architecture (2011)
  • Author(s): Yamamoto S, Tsuda H, Shimazaki H, Takano M, Yoshikawa T, Kuzuya K, Tsuda H, Kurachi H, Kigawa J, Kikuchi y Sugiyama T, Matsubara O
  • Journal Title: Int J Gynecol Pathol Volume: 31 Pages: 116-124
  • Peer Reviewed
• [Journal Article] Validation of the histological grading for ovarian clear-cell adenocarcinoma : a retrospective multi-institutional study of japan clear cell carcinoma study group. (2011)
  • Author(s): Yamamoto S, Kasajima A, Takano M, Yaegashi N, Suzuki M, Kuzuya K, Kigawa J, Tsuda H, Kurachi H, Kikuchi Y, Sugiyama T, Tsuda H, Motiya T.
  • Journal Title: Int J Gynecol Path Volume: 30(2) Pages: 129-138
  • Peer Reviewed
• [Journal Article] Clear cell adenocarcinoma with a component of poorly differentiated histology : a poor prognostic subgroup of ovarian clear cell adenocarcinoma (2011)
  • Author(s): Yamamoto S, Tsuda H, Shimazaki H, Takano M, Yoshikawa T, Kuzuya K, Tsuda H, Kurachi H, Kigawa J, Kikuchi Y, Sugiyama T.
  • Journal Title: Int J Gynecol Path Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Molecular mechanism of the inhibition of estradiol-induced endometrial epithelial cell proliferation by clomiphene citrate (2010)
  • Author(s): Amita M, Takahashi T, Ohta T, Takata K, Kurachi H
  • Journal Title: Endocrinology Volume: 151(1) Issue: 1 Pages: 394-405
  • DOI: 10.1210/en.2009-0721
  • Peer Reviewed
• [Journal Article] Histopathological prognostic factors predicting para-aortic lymph node metastasis in patients with endometrioid uterine cancer (2010)
  • Author(s): Karube Y, Kurachi H
  • Journal Title: Gynecol Oncol Volume: 118(2) Issue: 2 Pages: 151-154
  • DOI: 10.1016/j.ygyno.2010.05.004
  • Peer Reviewed
• [Journal Article] Molecular mechanism of the inhibition of estradiol-induced endometrial epithelial cell proliferation by clomiphene citrate. (2010)
  • Author(s): Amita M, Takahashi T, Tsutsumi S, Ohta T, Takata K, Henmi N, Hara S, Igarashi K, Takahashi K, Kurachi H.
  • Journal Title: Endocrinology Volume: 151 Pages: 394-405
  • Peer Reviewed
• [Journal Article] Use of monoclonal antibodies in the treatment of ovarian cancer. (2010)
  • Author(s): Mabuchi S, Morishige K, Kimura T
  • Journal Title: Curr Opin Obstet Gynecol Volume: 22 Pages: 3-8
  • Peer Reviewed
• [Journal Article] Concurrent Weekly Nedaplatin, External Beam Radiotherapy and High-Dose-Rate Brachytherapy in Patients with FIGO Stage Tub Cervical Cancer : A Comparison with a Cohort Treated by Radiotherapy Alone. (2010)
  • Author(s): Mabuchi S, Ugaki H, Isohashi F, Yoshioka Y, Temma K, Yada-Hashimoto N, Takeda T, Yamamoto T, Yoshino K, Nakajima R, Kuragaki C, Morishige K, Enomoto T, Inoue T, Kimura T.
  • Journal Title: Gynecol Obstet Invest Volume: 69 Pages: 224-232
  • Peer Reviewed
• [Journal Article] Squamous cell carcinoma of the uterine cervix producing granulocyte colony-stimulating factor : a report of 4 cases and a review of the literature. (2010)
  • Author(s): Matsumoto Y, Mabuchi S, Muraji M, Morii E, Kimura T
  • Journal Title: Int J Gynecol Cancer Volume: 20 Pages: 417-421
  • Peer Reviewed
• [Presentation] 2年前の膀胱癌由来と思われる腹膜偽粘液腫の1例 (2013)
  • Author(s): 小島原敬信, 高橋俊文, 松尾幸城, 松村創平, 小幡美由紀, 松川淳, 倉智博久
  • Organizer: 第65回日本産科婦人科学会
  • Place of Presentation: 札幌市教育文化会館(札幌市)
• [Presentation] 2年前の膀胱癌由来と思われる腹膜偽粘液腫の1例. (2013)
  • Author(s): 小島原敬信, 高橋俊文, 松尾幸城, 松村創平, 小幡美由紀, 松川 淳, 倉智博久.
  • Organizer: 第65回日本産科婦人科学会
  • Place of Presentation: 札幌市教育文化会館（札幌市）
• [Presentation] 卵管悪性腫瘍の臨床的解析東北婦人科腫瘍研究会後方視的検討 (2012)
  • Author(s): 横山良仁, 倉智博久 (他13名)
  • Organizer: 第50回日本癌治療学会
  • Place of Presentation: パシフィコ横浜 (横浜市)
• [Presentation] 卵管悪性腫瘍の臨床的解析 東北婦人科腫瘍研究会後方視的検討. (2012)
  • Author(s): 横山良仁,二神真行,高取恵里子,杉山 徹,大槻健郎,八重樫伸生,藤本俊郎,寺田幸弘,佐藤直樹,小島原敬信,倉智博久,西山 浩,藤森敬也,田勢 亨,水沼英樹.
  • Organizer: 第50回日本癌治療学会
  • Place of Presentation: 横浜
• [Presentation] 術後に卵巣小細胞癌と子宮体癌の重複癌と判明した一例. (2012)
  • Author(s): 松村創平,高橋俊文,倉智博久.
  • Organizer: 第133回東北連合産科婦人科学会
  • Place of Presentation: 秋田
• [Presentation] 再発卵巣癌に対するリポソーマルドキソルビシン単剤療法における手足症候群および口内炎予防のためのサポーティブケアに関する多施設共同研究 (2011)
  • Author(s): 島田宗昭, 倉智博久 (他26名)
  • Organizer: 第63回日本産科婦人科学会 大阪国際会議場
  • Place of Presentation: リーガロイヤルホテル大阪(大阪市)
• [Presentation] 術後多発肺転移をきたした類上皮栄養膜細胞腫瘍の一症例 (2011)
  • Author(s): 須藤毅, 小島原敬信, 成味恵, 清野学, 高橋一広, 中原健次, 倉智博久, 本山悌一
  • Organizer: 第50回日本産科腫瘍学会
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] Malignant transformation of mature cystic teratoma25症例の予後調査と術後療法についての検討 (2011)
  • Author(s): 徳永英樹, 倉智博久, 藤本俊郎, 田勢亨, 横山良仁, 小見英夫, 西山浩, 杉山徹, 八重樫伸生
  • Organizer: 第63回日本産科婦人科学会
  • Place of Presentation: リーガロイヤルホテル大阪
• [Presentation] 再発卵巣癌に対するリポソーマルドキソルビシン単剤療法における手足症候群および口内炎予防のためのサポーティブケアに関する多施設共同研究 (2011)
  • Author(s): 島田宗昭, 紀川純三, 佐藤慎也, 八重樫伸生, 高野忠夫, 大槻健郎, 鈴木光明, 藤原寛行, 藤原恵一, 長尾昌二, 水沼英樹, 横山良仁, 倉智博久, 中原健次, 杉山徹, 熊谷晴介, 滝澤憲, 岩瀬春子, 竹原和宏, 中村紘子, 日浦昌道, 白山裕子, 吉川裕之, 佐藤豊実, 落合和徳, 山田恭輔, 波多江正紀, 中村俊昭
  • Organizer: 第63回日本産科婦人科学会
  • Place of Presentation: リーガロイヤルホテル大阪
• [Presentation] 腹式広汎性子宮頸部摘出術の安全性の検討 (2011)
  • Author(s): 大槻健郎, 西山浩, 藤本俊郎, 横山良仁, 田勢亨, 杉山徹, 倉智博久, 八重樫伸生
  • Organizer: 第63回日本産科婦人科学会
  • Place of Presentation: リーガロイヤルホテル大阪
• [Presentation] 卵巣明細胞腺癌に対する新規抗癌剤Trabectedinの抗腫瘍効果および耐性化のメカニズムの検討 (2011)
  • Author(s): 馬淵誠士, 久松武志, 河瀬千晶, 林正美, 澤田健二郎, 木村正
  • Organizer: 第63回日本産科婦人科学会
  • Place of Presentation: リーガロイヤルホテル大阪
• [Presentation] 子宮頸がん検診と予防ワクチンについて (2010)
  • Author(s): 倉智博久
  • Organizer: 「子宮頸がん」市民公開講座招待講演
  • Place of Presentation: 大阪市緒方ビル
  • Year and Date: 2010-06-18
• [Presentation] GFP結合ERαを用いた子宮内膜細胞におけるエストロゲン, 抗エストロゲン作用の可視化 (2010)
  • Author(s): 網田光善, 高橋俊文, 原周一郎, 五十嵐秀樹, 倉智博久
  • Organizer: 第62回日本産科婦人科学会
  • Place of Presentation: 東京国際フォーラム(東京都)
• [Presentation] 化学療法後の卵巣癌再発巣におけるAktとERKの活性化は予後良好因子である (2010)
  • Author(s): 清野 学, 太田剛, 高橋俊文, 逸見典子, 須藤毅, 本山悌一, 倉智博久
  • Organizer: 第62回日本産科婦人科学会
  • Place of Presentation: 東京国際フォーラム(東京都)
• [Presentation] 子宮癌肉腫に対するパクリタキセル・カルボプラチン併用術後補助化学療法の第II相試験 (2010)
  • Author(s): 高野忠夫, 大槻健郎, 吉永浩介, 永瀬智, 新倉仁, 伊藤潔, 八重樫伸生, 田勢亨, 中原健次, 倉智博久, 横山良仁, 水沼英樹, 竹原和宏, 野村弘行, 青木大輔, 中山裕樹, 藤本俊郎, 田中俊誠, 大竹秀幸, 片渕秀隆
  • Organizer: 第48回日本婦人科腫瘍学会
  • Place of Presentation: つくば国際会議場
• [Presentation] GFP-ERαを用いた子宮内膜細胞におけるエストロゲン・抗エストロゲン作用の可視化 (2010)
  • Author(s): 網田光善, 高橋俊文, 原周一郎, 五十嵐秀樹, 倉智博久
  • Organizer: 第55回日本生殖医学会
  • Place of Presentation: 徳島あわぎんホール
• [Presentation] 再発危険因子を有する子宮頚癌(I-II期)に対するネダプラチンを用いた術後Concurrent Chemoradiotherapy(CCRT)の意義 (2010)
  • Author(s): 馬淵誠士、森重健一郎、榎本隆之、木村正
  • Organizer: 第62回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] mTORをターゲットとした卵巣明細胞腺癌の治療戦略 (2010)
  • Author(s): 河瀬千晶、馬淵誠士、澤田健二郎、森重健一郎、木村正、大道正英、矢本希夫、伊藤公彦、西尾幸浩
  • Organizer: 第62回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] mTOR is a promising therapeutic target both in cisplatin-sensitive and cisplatin-resistant clear cell carcinoma of the ovary. (2010)
  • Author(s): Mabuchi, S.
  • Organizer: 13th Biennial Meeting of the International Gynecological Cancer Society
  • Place of Presentation: Prague
• [Book] 産婦人科手術・処置とトラブル対処法 (2010)
  • Author(s): 中原健次, 小島原敬信, 倉智博久
  • Total Pages: 324
  • Publisher: 婦人科手術 開腹手術 [キーワード:広汎性子宮全摘術] ここが聞きたい
• [Book] 産婦人科手術・処置とトラブル対処法 (2010)
  • Author(s): 中原健次, 太田剛, 倉智博久
  • Total Pages: 324
  • Publisher: 婦人科手術 開腹手術 [キーワード:卵巣腫瘍摘出術] ここが聞きたい
• [Remarks]
  • URL: http://www.id.yamagata-u.ac.jp/ObGyn/index.html
• [Remarks]
  • URL: http://www.id.yamagata-u.ac.jp/ObGyn/index.html
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/gyne/www/index.html