Influenza surveillance in Myanmar: investigations on emergence of drug resistant strains and clinical pictures of H1N1pdm09

• Project/Area Number: 22406013
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Virology
• Research Institution: Niigata University
• Principal Investigator: SAITO Reiko 新潟大学, 医歯学系, 教授 (30345524)
• Co-Investigator(Kenkyū-buntansha): NAITO Makoto 新潟大学, 医歯学系, 教授 (30045786) ; HASEGAWA Go 新潟大学, 医歯学系, 准教授 (90251800) ; FUJII Masahiro 新潟大学, 医歯学系, 教授 (30183099) ; OOIE Masayasu 新潟大学, 医歯学系, 助教 (70108017)
• Co-Investigator(Renkei-kenkyūsha): OOHASHI Riuko 新潟大学, 医歯学総合病院, 助教 (20447600) ; SAITO Takehiko 独立行政法人農業・食品産業技術総合研究機構, 動物衛生研究所, 上席研究員 (00263393)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: インフルエンザ / ミャンマー / 流行時期 / ウイルス遺伝子解析 / ウイルス進化 / 薬剤耐性 / 新型インフルエンザ / 国際研究 / 日本 / 感染症疫学 / 分子疫学 / HA遺伝子 / NA遺伝子 / オセルタミビル / 遺伝子解析 / サーベイランス

Research Abstract

We investigated influenza viruses circulated in the community in Yangon and Nay Pyi Taw in Myanmar during FY 2010-2012. In total we isolated 643 influenza viruses during three years, and 257 were A/H1N1pdm09, 182 A/H3N2, and 204 B. Epidemic season in Myanmar was from May to November during the rainy season. For A/H3N2 and B, new genetic variants appeared a half year earlier than in Japan. Drug resistant surveillance showed that there was no oseltamivir resistant H274Y mutated strains during the years. We identified two drug resistant influenza B that conferred resistance to zanamivir and lanaimivir, and possessed I248G mutation in the NA gene.

Research Products

• [Journal Article] Delayed emergence of oseltamivir-resistant seasonal influenza A(H1N1) and pandemic influenza A(H1N1)pdm09 viruses in Myanmar (2012)
  • Author(s): Dapat C, Saito R, Kyaw Y, Myint YY, Oo HN, 00 KY, Naito M, Hasegawa G, Dapat IC, Suzuki H.
  • Journal Title: Influenza and other respiratory viruses Volume: 5 Issue: 5 Pages: 1-6
  • DOI: 10.1111/irv.12030
  • Peer Reviewed
• [Journal Article] Identification of oseltamivir resistance among pandemic and seasonal influenza A (H1N1) viruses by an His275Tyr genotyping assay using the cycling probe method (2011)
  • Author(s): Suzuki Y, Saito R, Sato I, Zaraket H, Nishikawa M, Tamura T, Dapat C, Caperig-Dapat I, Baranovich T, Suzuki T, Suzuki H
  • Journal Title: J Clin Microbiol Volume: 49(1) Pages: 125-30
  • Peer Reviewed
• [Journal Article] Identification of oseltamivir resistance among pandemic and seasonal influenza A(RIN1)viruses by an His275Tyrgenotyping assay using the cycling probe method. (2011)
  • Author(s): Suzuki Y, Saito R, Sato I, et al
  • Journal Title: J Clin Microbiol. Volume: 49巻 Pages: 125-130
  • Peer Reviewed
• [Journal Article] Genetic makeup of amantadine-resistant and oseltamivir- resistant human influenza A/H1N1 viruses (2010)
  • Author(s): Zaraket H, Saito R, Suzuki Y, Baranovich T, Dapat C, Caperig-Dapat I, Suzuki H
  • Journal Title: J Clin Microbiol Volume: 48(4) Pages: 1085-92
  • Peer Reviewed
• [Journal Article] New genotypes within respiratory syncytial virus group B genotype BA in Niigata, Japan (2010)
  • Author(s): Dapat IC, Shobugawa Y, Sano Y, Saito R, Sasaki A, Suzuki Y, Kumaki A, Zaraket H, Dapat C, Oguma T, Yamaguchi M, Suzuki H
  • Journal Title: J Clin Microbiol Volume: 48(9) Pages: 3423-7
  • Peer Reviewed
• [Journal Article] Reduced effectiveness of oseltamivir in children infected with oseltamivir-resistant influenza A (H1N1) viruses with His275Tyr mutation (2010)
  • Author(s): Saito R, Sato I, Suzuki Y, Baranovich T, Matsuda R, Ishitani N, Dapat C, Dapat IC, Zaraket H, Oguma T, Suzuki H
  • Journal Title: Pediatr Infect Dis J Volume: 29(10) Pages: 898-904
  • Peer Reviewed
• [Journal Article] Rare influenza A (H3N2) variants with reduced sensitivity to antiviral drugs (2010)
  • Author(s): Dapat C, Suzuki Y, Saito R, Kyaw Y, Myint YY, Lin N, Oo HN, Oo KY, Win N, Naito M, Hasegawa G, Dapat IC, Zaraket H, Baranovich T, Nishikawa M, Saito T, Suzuki H
  • Journal Title: Emerg Infect Dis Volume: 16(3) Pages: 493-6
  • Peer Reviewed
• [Journal Article] Genomic events contributing to the high prevalence of amantadine-resistant influenza A/H3N2. (2010)
  • Author(s): Zaraket H, Saito R, Suzuki Y, Suzuki Y, Caperig-Dapat I, et.al
  • Journal Title: Antivir Ther. Volume: 15巻 Pages: 307-319
  • Peer Reviewed
• [Journal Article] Reduced effectiveness of oseltamivir in children infected with oseltamivir-resistant influenza A(RIN1)viruses with His275Tyr mutation. (2010)
  • Author(s): Saito R, Sato I, Suzuki Y, Baranovich T, Matsuda R, et.al
  • Journal Title: Pediatr Infect Dis J. Volume: 29巻 Pages: 898-904
  • Peer Reviewed
• [Presentation] Epidemiology of Influenza in Myanmar (2013)
  • Author(s): Reiko Saito, Makoto Naito, Yadanar Kyaw, Yi Yi Myint, Khin Yi Oo, Go Hasegawa,Clyde Dapat, Isolde Dapat, Yugo Shobugawa, Kousuke Saito, Hiroshi Suzuki
  • Organizer: 新興・再興感染症に関するアジア・アフリカリサーチフォーラムAARF2013
  • Place of Presentation: 東京都
• [Presentation] Genetic Characterization of Human Influenza A (H3N2) Virus in Myanmar, 2007-2011 (2013)
  • Author(s): Clyde Dapat、齋藤玲子、齋藤孔良、Isolde Dapat、近藤 大貴、鈴木宏
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪市
• [Presentation] Epidemiology of Influenza in Myanmar (2013)
  • Author(s): Reiko Saito, Makoto Naito, Yadanar Kyaw, Yi Yi Myint, Khin Yi Oo, Go Hasegawa,Clyde Dapat, Isolde Dapat, Yugo Shobugawa, Kousuke Saito, Hiroshi Suzuki
  • Organizer: 新興・再興感染症に関するアジア・アフリカリサーチフォーラム AARF2013
  • Place of Presentation: 東京医科歯科大学鈴木章夫講堂（東京都）
• [Presentation] Genetic Characterization of Human Influenza A (H3N2) Virus in Myanmar, 2007-2011 (2012)
  • Author(s): Dapat Clyde、齋藤 玲子、齋藤 孔良、Dapat Isolde、近藤 大貴、鈴木 宏
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪（大阪府）
• [Presentation] 薬剤耐性インフルエンザとその臨床 (2011)
  • Author(s): 齋藤玲子
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島市
  • Year and Date: 2011-11-07
• [Presentation] Prevalence of antiviral drug-resistant influenza in Myanmar from 2007 to 2010 (2011)
  • Author(s): Clyde Dapat, Reiko Saito, Isolde Dapat, Yasushi Suzuki, Makoto Naito, Yadanar Kyaw, Yi Yi Myint, Hiroshi Suzuki
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-15
• [Presentation] PREVALENCE OF ANTIVIRAL DRUG-RESISTANT INFLUENZA A VIRUSES IN MYANMAR FROM 2007 TO 2010 (2011)
  • Author(s): Clyde Dapat, Reiko Saito, Isolde Dapat, Yasushi Suzuki, Makoto Naito, Yadanar Kyaw, Yi Yi Myint, Hiroshi Suzuki
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 北海道(札幌コンベンションセンター)
  • Year and Date: 2011-09-15
• [Book] 黄金のパゴダミャンマーの光と影 (2013)
  • Author(s): 内藤眞
  • Total Pages: 231
  • Publisher: 新潟日報事業所
• [Book] 黄金のパゴダ ミャンマーの光と影 (2013)
  • Author(s): 内藤 眞
  • Total Pages: 231
  • Publisher: 新潟日報事業所
• [Remarks] 新潟大学大学院医歯学総合研究科実験病理学(第二病理)
  • URL: http://www.med.niigata-u.ac.jp/pa2/katudouhoukoku.htm
• [Remarks] ミャンマー訪問記録
• [Remarks] 第20回目の訪緬 －激動のミャンマー、変らぬミャンマー－（その１）
  • URL: http://www.med.niigata-u.ac.jp/pa2/myanmar2012akipart1.htm
• [Remarks] 第20回目の訪緬 －激動のミャンマー、変らぬミャンマー－（その2）
  • URL: http://www.med.niigata-u.ac.jp/pa2/myanmar2012akipart2.htm