Role of growth cone autonomy and translational trans-acting factors in axon guidance

• Project/Area Number: 22500336
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Yokohama City University
• Principal Investigator: SASAKI Yukio 横浜市立大学, 医学研究科, 特任准教授 (10295511)
• Co-Investigator(Renkei-kenkyūsha): GOSHIMA Yoshio 横浜市立大学, 医学研究科, 教授 (00153750)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 分子・細胞・神経生物学 / 成長円錐 / 翻訳調節 / マイクロ RNA / 横浜市 / 神経生物学 / マイクロRNA

Research Abstract

We have investigated two mRNA-binding, translational trans-acting factors to regulate local translation in axonal growth cones in neurons. We demonstratedthat Zipcode-Binding Protein 1 (ZBP1), an mRNA-binding protein, regulates translation of β-actin mRNA, and then direction of axonal extension, via phosphorylation induced by Brain-Derived Neurotrophic Factor (BDNF). We also found that microRNAs (miRNAs), non-coding RNAs bind to mRNA, exist in growth cones. We indicate the possibility that axonal miRNAs are involved in axonal extension regulated by BDNF.

Research Products

• [Journal Article] Regulation of neurite outgrowth mediated by localized phosphorylation of protein translational factor eEF2 in growth cones (2013)
  • Author(s): Iketani M., Iizuka A., Sengoku K., Kurihara Y., Nakamura F., Sasaki Y., Sato Y., Yamane M., Matsushita M., Nairn A.C., Takamatsu K., Goshima Y., Takei K.
  • Journal Title: Dev. Neurosci Volume: 73 Issue: 3 Pages: 230-246
  • DOI: 10.1002/dneu.22058
  • Peer Reviewed
• [Journal Article] Class 3 semaphorins as a therapeutic target (2012)
  • Author(s): Goshima Y., Sasaki Y., Yamashita N., Nakamura F
  • Journal Title: Expert Opin.Ther. Targets Volume: 16 Issue: 9 Pages: 933-944
  • DOI: 10.1517/14728222.2012.710201
  • Peer Reviewed
• [Journal Article] GSK3β/axin-1/β-catenin complex is involved in semaphorin3A signaling. (2012)
  • Author(s): Hida T., Yamashita N., Usui H., Nakamura F.Sasaki Y., Kikuchi A.Goshima Y.
  • Journal Title: J. Neurosci Volume: 32 Issue: 35 Pages: 11905-11918
  • DOI: 10.1523/jneurosci.6139-11.2012
  • Peer Reviewed
• [Journal Article] 神経軸索・成長円錐における局所翻訳制御と機能調節 (2012)
  • Author(s): 佐々木 幸生、五嶋 良郎
  • Journal Title: 細胞工学 Volume: 31 Pages: 677-682
• [Journal Article] CRMP5(collapsin response mediator protein 5) regulates dendritic development and synaptic plasticity in the cerebellar Purkinje cells (2011)
  • Author(s): Yamashita N, Mosinger B, Roy A, Miyazaki M, Ugajin K, Nakamura F, Sasaki Y, Yamaguchi K, Kolattukudy P, Goshima Y.
  • Journal Title: Journal of Neuroscience Volume: 31(5) Issue: 5 Pages: 1773-79
  • DOI: 10.1523/jneurosci.5337-10.2011
  • Peer Reviewed
• [Journal Article] Regulation of chemotropic guidance of nerve growth cones by microRNA (2011)
  • Author(s): Han, L., Wen, Z., Lynn, R. C., Baudet, M. L., Holt, C. E., Sasaki, Y., Bassell, G. J., Zheng, J. Q
  • Journal Title: Mol. Brain Volume: 4 Issue: 1 Pages: 40-40
  • DOI: 10.1186/1756-6606-4-40
  • Peer Reviewed
• [Journal Article] Phosphorylation of zipcode binding protein is required for BDNF signaling of local β-actin synthesis and growth cone turning (2011)
  • Author(s): Sasaki, Y., Welshhans, K., Wen, Z., Yao, J., Xu, M., Goshima, Y., Zheng, J. Q., Bassell, G. J.
  • Journal Title: J. Neurosci Volume: 30 Issue: 28 Pages: 9349-9358
  • DOI: 10.1523/jneurosci.0499-10.2010
  • Peer Reviewed
• [Journal Article] CRMP5(Collapsin Response Mediator Protein 5) Regulates Dendritic Development and Synaptic Plasticity in the Cerebellar Purkinje Cells. (2011)
  • Author(s): Yamashita, N., Mosinger, B., Roy, A., Miyazaki, M., Ugajin, K., Nakamura, F., Sasaki, Y., Yamaguchi, K., Kolattukudy, P., Goshima, Y.
  • Journal Title: J.Neurosci. Volume: 31 Pages: 1773-1779
  • Peer Reviewed
• [Journal Article] Phosphorylation of zipcode binding protein is required for BDNF signaling of local β-actin synthesis and growth cone turning. (2010)
  • Author(s): Sasaki, Y., Welshhans, K., Wen, Z., Yao, J., Xu, M., Goshima, Y., Zheng, J.Q., Bassell, G.J.
  • Journal Title: J.Neurosci. Volume: 30 Pages: 9349-9358
  • Peer Reviewed
• [Presentation] 翻訳トランス因子による軸索局所翻訳制御と神経疾患との関 (2013)
  • Author(s): 佐々木 幸生
  • Organizer: 連日本生理学会(招待講演)
  • Place of Presentation: タワーホール船堀(東京都)。
  • Year and Date: 2013-03-28
• [Presentation] 翻訳トランス因子による軸索局所翻訳制御と神経疾患との関連 (2013)
  • Author(s): 佐々木 幸生
  • Organizer: 日本生理学会
  • Place of Presentation: タワーホール船堀（東京）
  • Invited
• [Presentation] 神経軸索におけるマイクロ RNA オミクスにより同定された miRNA-720 は神経栄養因子による軸索伸長に関与する (2012)
  • Author(s): 佐々木 幸生.Xing, L.、Gross C.、五嶋 良郎, Bassell, G. J
  • Organizer: 日本 RNA 学会
  • Place of Presentation: 東北大学百周年記念会館川内萩ホール(宮城県)
  • Year and Date: 2012-07-20
• [Presentation] Identification of axonal microRNAs reveals involvement of miR-720 in neurotrophin-regulated axonal growth (2012)
  • Author(s): Sasaki Y., Xing L., Gross C., Goshima, Y., Bassell G.J.
  • Organizer: The 22nd CDB Meeting, RNA Sciences in Cell and Developmental Biology II
  • Place of Presentation: 理化学研究所 発生・再生科学総合研究センター(兵庫県)
  • Year and Date: 2012-06-13
• [Presentation] Identification of axonal microRNAs reveals involvement of miR-720 in neurotrophin-regulated axonal growth (2012)
  • Author(s): Sasaki Y,Xing L, Gross C, Goshima Bassell GJ
  • Organizer: The 22nd CDB Meeting, RNA Sciences in Cell and Developmental Biology II
  • Place of Presentation: 理研CDB（兵庫）
• [Presentation] 神経軸索におけるマイクロRNAオミクスにより同定されたmiRNA-720は神経栄養因子による軸索伸長に関与する (2012)
  • Author(s): 佐々木 幸生、Lei Xing、Christina Gross、五嶋 良郎, Gary J. Bassell
  • Organizer: 日本RNA学会
  • Place of Presentation: 東北大学（宮城）
• [Presentation] Brain-derived neurotrophic factor regulates localization of microRNA-720 in growth cones (2011)
  • Author(s): 佐々木 幸生, Xing, L., Gross, C.五嶋 良郎, Bassell, G. J
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府)。
  • Year and Date: 2011-09-24
• [Presentation] Brain-derived neurotrophic factor regulates localization of microRNA-720 in growth cones (2011)
  • Author(s): 佐々木幸生, Xing, L., Gross, C., 五嶋良郎., Bassell, G.J.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-09-24
• [Presentation] Brain-derived neurotrophic factor regulates localization of microRNA-720 in growth cones (2011)
  • Author(s): Sasaki, Y., Xing, L., Gross, C., Goshima, Y., and Bassell, G. J
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-09-16
• [Presentation] Brain-derived neurotrophic factor regulates localization of microRNA-720 in growth cones (2011)
  • Author(s): Sasaki, Y., Xing, L., Gross, C., Goshima, Y., Bassell, G.J.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: パシフィコ横浜(横浜)
  • Year and Date: 2011-09-16
• [Presentation] Profiling of microRNAs enriched in axons (2010)
  • Author(s): Sasaki Y., Xing, L., Gross, C., Goshima, Y., Bassell, G.J
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(兵庫県) 。
  • Year and Date: 2010-09-04
• [Presentation] Profiling of microRNAs enriched in axons (2010)
  • Author(s): Yukio Sasaki, Lei Xing, Christina Gross, Yoshio Goshima, Gary J.Bassell
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸国際会議場(神戸)
  • Year and Date: 2010-09-04