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Role of growth cone autonomy and translational trans-acting factors in axon guidance

Research Project

Project/Area Number 22500336
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionYokohama City University

Principal Investigator

SASAKI Yukio  横浜市立大学, 医学研究科, 特任准教授 (10295511)

Co-Investigator(Renkei-kenkyūsha) GOSHIMA Yoshio  横浜市立大学, 医学研究科, 教授 (00153750)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords分子・細胞・神経生物学 / 成長円錐 / 翻訳調節 / マイクロ RNA / 横浜市 / 神経生物学 / マイクロRNA
Research Abstract

We have investigated two mRNA-binding, translational trans-acting factors to regulate local translation in axonal growth cones in neurons. We demonstratedthat Zipcode-Binding Protein 1 (ZBP1), an mRNA-binding protein, regulates translation of β-actin mRNA, and then direction of axonal extension, via phosphorylation induced by Brain-Derived Neurotrophic Factor (BDNF). We also found that microRNAs (miRNAs), non-coding RNAs bind to mRNA, exist in growth cones. We indicate the possibility that axonal miRNAs are involved in axonal extension regulated by BDNF.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (21 results)

All 2013 2012 2011 2010

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (12 results) (of which Invited: 1 results)

  • [Journal Article] Regulation of neurite outgrowth mediated by localized phosphorylation of protein translational factor eEF2 in growth cones2013

    • Author(s)
      Iketani M., Iizuka A., Sengoku K., Kurihara Y., Nakamura F., Sasaki Y., Sato Y., Yamane M., Matsushita M., Nairn A.C., Takamatsu K., Goshima Y., Takei K.
    • Journal Title

      Dev. Neurosci

      Volume: 73 Issue: 3 Pages: 230-246

    • DOI

      10.1002/dneu.22058

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Class 3 semaphorins as a therapeutic target2012

    • Author(s)
      Goshima Y., Sasaki Y., Yamashita N., Nakamura F
    • Journal Title

      Expert Opin.Ther. Targets

      Volume: 16 Issue: 9 Pages: 933-944

    • DOI

      10.1517/14728222.2012.710201

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] GSK3β/axin-1/β-catenin complex is involved in semaphorin3A signaling.2012

    • Author(s)
      Hida T., Yamashita N., Usui H., Nakamura F.Sasaki Y., Kikuchi A.Goshima Y.
    • Journal Title

      J. Neurosci

      Volume: 32 Issue: 35 Pages: 11905-11918

    • DOI

      10.1523/jneurosci.6139-11.2012

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 神経軸索・成長円錐における局所翻訳制御と機能調節2012

    • Author(s)
      佐々木 幸生、五嶋 良郎
    • Journal Title

      細胞工学

      Volume: 31 Pages: 677-682

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] CRMP5(collapsin response mediator protein 5) regulates dendritic development and synaptic plasticity in the cerebellar Purkinje cells2011

    • Author(s)
      Yamashita N, Mosinger B, Roy A, Miyazaki M, Ugajin K, Nakamura F, Sasaki Y, Yamaguchi K, Kolattukudy P, Goshima Y.
    • Journal Title

      Journal of Neuroscience

      Volume: 31(5) Issue: 5 Pages: 1773-79

    • DOI

      10.1523/jneurosci.5337-10.2011

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of chemotropic guidance of nerve growth cones by microRNA2011

    • Author(s)
      Han, L., Wen, Z., Lynn, R. C., Baudet, M. L., Holt, C. E., Sasaki, Y., Bassell, G. J., Zheng, J. Q
    • Journal Title

      Mol. Brain

      Volume: 4 Issue: 1 Pages: 40-40

    • DOI

      10.1186/1756-6606-4-40

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of zipcode binding protein is required for BDNF signaling of local β-actin synthesis and growth cone turning2011

    • Author(s)
      Sasaki, Y., Welshhans, K., Wen, Z., Yao, J., Xu, M., Goshima, Y., Zheng, J. Q., Bassell, G. J.
    • Journal Title

      J. Neurosci

      Volume: 30 Issue: 28 Pages: 9349-9358

    • DOI

      10.1523/jneurosci.0499-10.2010

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] CRMP5(Collapsin Response Mediator Protein 5) Regulates Dendritic Development and Synaptic Plasticity in the Cerebellar Purkinje Cells.2011

    • Author(s)
      Yamashita, N., Mosinger, B., Roy, A., Miyazaki, M., Ugajin, K., Nakamura, F., Sasaki, Y., Yamaguchi, K., Kolattukudy, P., Goshima, Y.
    • Journal Title

      J.Neurosci.

      Volume: 31 Pages: 1773-1779

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation of zipcode binding protein is required for BDNF signaling of local β-actin synthesis and growth cone turning.2010

    • Author(s)
      Sasaki, Y., Welshhans, K., Wen, Z., Yao, J., Xu, M., Goshima, Y., Zheng, J.Q., Bassell, G.J.
    • Journal Title

      J.Neurosci.

      Volume: 30 Pages: 9349-9358

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 翻訳トランス因子による軸索局所翻訳制御と神経疾患との関2013

    • Author(s)
      佐々木 幸生
    • Organizer
      連日本生理学会(招待講演)
    • Place of Presentation
      タワーホール船堀(東京都)。
    • Year and Date
      2013-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 翻訳トランス因子による軸索局所翻訳制御と神経疾患との関連2013

    • Author(s)
      佐々木 幸生
    • Organizer
      日本生理学会
    • Place of Presentation
      タワーホール船堀(東京)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 神経軸索におけるマイクロ RNA オミクスにより同定された miRNA-720 は神経栄養因子による軸索伸長に関与する2012

    • Author(s)
      佐々木 幸生.Xing, L.、Gross C.、五嶋 良郎, Bassell, G. J
    • Organizer
      日本 RNA 学会
    • Place of Presentation
      東北大学百周年記念会館川内萩ホール(宮城県)
    • Year and Date
      2012-07-20
    • Related Report
      2012 Final Research Report
  • [Presentation] Identification of axonal microRNAs reveals involvement of miR-720 in neurotrophin-regulated axonal growth2012

    • Author(s)
      Sasaki Y., Xing L., Gross C., Goshima, Y., Bassell G.J.
    • Organizer
      The 22nd CDB Meeting, RNA Sciences in Cell and Developmental Biology II
    • Place of Presentation
      理化学研究所 発生・再生科学総合研究センター(兵庫県)
    • Year and Date
      2012-06-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Identification of axonal microRNAs reveals involvement of miR-720 in neurotrophin-regulated axonal growth2012

    • Author(s)
      Sasaki Y,Xing L, Gross C, Goshima Bassell GJ
    • Organizer
      The 22nd CDB Meeting, RNA Sciences in Cell and Developmental Biology II
    • Place of Presentation
      理研CDB(兵庫)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 神経軸索におけるマイクロRNAオミクスにより同定されたmiRNA-720は神経栄養因子による軸索伸長に関与する2012

    • Author(s)
      佐々木 幸生、Lei Xing、Christina Gross、五嶋 良郎, Gary J. Bassell
    • Organizer
      日本RNA学会
    • Place of Presentation
      東北大学(宮城)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Brain-derived neurotrophic factor regulates localization of microRNA-720 in growth cones2011

    • Author(s)
      佐々木 幸生, Xing, L., Gross, C.五嶋 良郎, Bassell, G. J
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府)。
    • Year and Date
      2011-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Brain-derived neurotrophic factor regulates localization of microRNA-720 in growth cones2011

    • Author(s)
      佐々木幸生, Xing, L., Gross, C., 五嶋良郎., Bassell, G.J.
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Brain-derived neurotrophic factor regulates localization of microRNA-720 in growth cones2011

    • Author(s)
      Sasaki, Y., Xing, L., Gross, C., Goshima, Y., and Bassell, G. J
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Brain-derived neurotrophic factor regulates localization of microRNA-720 in growth cones2011

    • Author(s)
      Sasaki, Y., Xing, L., Gross, C., Goshima, Y., Bassell, G.J.
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Profiling of microRNAs enriched in axons2010

    • Author(s)
      Sasaki Y., Xing, L., Gross, C., Goshima, Y., Bassell, G.J
    • Organizer
      第33回日本神経科学大会
    • Place of Presentation
      神戸コンベンションセンター(兵庫県) 。
    • Year and Date
      2010-09-04
    • Related Report
      2012 Final Research Report
  • [Presentation] Profiling of microRNAs enriched in axons2010

    • Author(s)
      Yukio Sasaki, Lei Xing, Christina Gross, Yoshio Goshima, Gary J.Bassell
    • Organizer
      第33回日本神経科学大会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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