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Generation of multi-step, site-specific recombination system in vivo

Research Project

Project/Area Number 22500384
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionThe University of Tokyo

Principal Investigator

ICHISE Hirotake  東京大学, 医科学研究所, 助教 (10313090)

Co-Investigator(Kenkyū-buntansha) ICHISE Taeko  東京大学, 医科学研究所, 助教 (00396863)
YOSHIDA Nobuaki  東京大学, 医科学研究所, 教授 (10250341)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsCre/loxP / FLP/FRT / Dre/rox / 部位特異的組換え酵素 / マウス
Research Abstract

We identified the transgene integration site of the CGH4 transgenic mice that conditionally express H-Ras and EGFP in a variety of tissues in a Cre/loxP recombination-dependent manner (Ichise et al., 2010), generated new mouse lines using targeting vectors for the locus, and found that the locus is suitable for site-spesific recomibinase-mediated, binary expression of transgenes in an endothelial cell lineage. Targeted transgenesis and binary expression system using the locus in combination with the Rosa26 locus will be valuable for developing multi-step, site-specific recombination system in vivo.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (16 results)

All 2013 2012 2011 2010

All Journal Article (9 results) (of which Peer Reviewed: 6 results) Presentation (5 results) Book (2 results)

  • [Journal Article] PTB deficiency causes the loss of adherens junctions in the dorsal telencehalon and leads to lethal2013

    • Author(s)
      柴嵜孝幸
    • Journal Title

      Cerebral Cortex

      Volume: 一(掲載予定) Issue: 8 Pages: 1-12

    • DOI

      10.1093/cercor/bhs161

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ras/MAPK Signaling Modulates VEGFR-3 Expression through Ets-Mediated p300 Recruitment and Histone Acetylation on the Vegfr3 Gene in Lymphatic Endothelial Cells.2012

    • Author(s)
      Ichise, T., Yoshida, N., Ichise, H.
    • Journal Title

      PLoS One.

      Volume: 7 Issue: 12 Pages: e51639-e51639

    • DOI

      10.1371/journal.pone.0051639

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Y.Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells.2012

    • Author(s)
      Osada, M., Inoue, O., Ding, G., Shirai, T., Ichise, H., Hirayama, K., Takano, K., Yatomi, Y., Hirashima, M., Fujii, H., Suzuki-Inoue, K., Ozaki
    • Journal Title

      The Journal of biological chemistry.

      Volume: 287 Issue: 26 Pages: 22241-22252

    • DOI

      10.1074/jbc.m111.329987

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11(p58) in mouse embryonic stem cells2011

    • Author(s)
      Ohno S, Shibayama M, Sato M, Tokunaga A, Yoshida N
    • Journal Title

      Cell Cycle

      Volume: 10(21) Issue: 21 Pages: 3706-3713

    • DOI

      10.4161/cc.10.21.17903

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] N.Fbxl10/Kdm2b deficiency accelerates neural progenitor cell death and leads to exencephaly.2011

    • Author(s)
      Fukuda, T., Tokunaga, A., Sakamoto, R., Yoshida
    • Journal Title

      Molecular and cellular neurosciences.

      Volume: 46 Issue: 3 Pages: 614-624

    • DOI

      10.1016/j.mcn.2011.01.001

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] N., Miki, H.Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive Dishevelled protein.2010

    • Author(s)
      Funato, Y., Terabayashi, T., Sakamoto, R., Okuzaki, D., Ichise, H., Nojima, H.,Yoshida
    • Journal Title

      Current biology.

      Volume: 20 Issue: 21 Pages: 1945-1952

    • DOI

      10.1016/j.cub.2010.09.065

    • Related Report
      2012 Final Research Report
  • [Journal Article] Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of flightless-I to myeloid differentiation primary response gene (88).2010

    • Author(s)
      Hayashi, T., Funato, Y., Terabayashi, T., Morinaka, A., Sakamoto, R., Ichise, H., Fukuda, H., Yoshida, N., Miki, H.
    • Journal Title

      The Journal of biological chemistry.

      Volume: 285 Issue: 24 Pages: 18586-18593

    • DOI

      10.1074/jbc.m110.106468

    • Related Report
      2012 Final Research Report
  • [Journal Article] リンパ管新生におけるRasの役割2010

    • Author(s)
      市瀬 広武
    • Journal Title

      リンパ学

      Volume: 33 Pages: 102-106

    • URL

      http://mol.medicalonline.jp/archive/search?jo=ed6lymph&ye=2010&vo=33&issue=2

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Journal Article] Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of in active dishevelled protein.2010

    • Author(s)
      Funato Y, Terabayashi T, Sakamoto R, Okuzaki D, Ichise H, Nojima H, Yoshida N, Miki H.
    • Journal Title

      Current Biology

      Volume: 20 Pages: 1945-1952

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 先進的医学研究のための遺伝子改変動物研究コンソーシアムの概要2012

    • Author(s)
      吉田 進昭
    • Organizer
      第85回日本生化学会大会フォーラム「わが国における遺伝子改変動物研究コンソーシアムの取組みと現状」
    • Place of Presentation
      福岡国際会議場(福岡)
    • Year and Date
      2012-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] A Modulatory Role of Ras in VEGFR-3 Signalling during Normal and Pathological Lymphangiogenesis"2011

    • Author(s)
      Hirotake Ichise
    • Organizer
      第32回日本炎症・再生医学会シンポジウム "Tumor Microenvironment and Inflammation"
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2011-06-02
    • Related Report
      2012 Final Research Report
  • [Presentation] A Modulatory Role of Ras in VEGFR-3 Signalling during Normal and Pathological Lymphangiogenesis2011

    • Author(s)
      Hirotake Ichise
    • Organizer
      第32回日本炎症・再生医学会
    • Place of Presentation
      国立京都国際会館(京都)(招待講演)
    • Year and Date
      2011-06-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] リンパ管新生における Ras の役割2010

    • Author(s)
      市瀬 広武
    • Organizer
      第34回日本リンパ学会総会
    • Place of Presentation
      東京大学山上会館(東京)
    • Year and Date
      2010-06-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Role of Ras in lymphangiogenesis"Gordon Research Conference 2010,2010

    • Author(s)
      Hirotake Ichise
    • Organizer
      Molecular Mechanisms in Lymphatic Function & Disease
    • Place of Presentation
      Lucca (Barga), Italy(招待講演)
    • Year and Date
      2010-06-15
    • Related Report
      2012 Final Research Report
  • [Book] よくわかるリンパ浮腫のすべて2011

    • Author(s)
      市瀬 広武(光嶋 勲 編著)
    • Total Pages
      6
    • Publisher
      永井書店
    • Related Report
      2012 Final Research Report
  • [Book] よくわかるリンパ浮腫のすべて(分担執筆)2011

    • Author(s)
      市瀬広武(光嶋勲編著)
    • Total Pages
      6
    • Publisher
      永井書店
    • Related Report
      2011 Annual Research Report

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Published: 2010-11-30   Modified: 2019-07-29  

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