Generation of multi-step, site-specific recombination system in vivo

• Project/Area Number: 22500384
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: The University of Tokyo
• Principal Investigator: ICHISE Hirotake 東京大学, 医科学研究所, 助教 (10313090)
• Co-Investigator(Kenkyū-buntansha): ICHISE Taeko 東京大学, 医科学研究所, 助教 (00396863) ; YOSHIDA Nobuaki 東京大学, 医科学研究所, 教授 (10250341)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: Cre/loxP / FLP/FRT / Dre/rox / 部位特異的組換え酵素 / マウス

Research Abstract

We identified the transgene integration site of the CGH4 transgenic mice that conditionally express H-Ras and EGFP in a variety of tissues in a Cre/loxP recombination-dependent manner (Ichise et al., 2010), generated new mouse lines using targeting vectors for the locus, and found that the locus is suitable for site-spesific recomibinase-mediated, binary expression of transgenes in an endothelial cell lineage. Targeted transgenesis and binary expression system using the locus in combination with the Rosa26 locus will be valuable for developing multi-step, site-specific recombination system in vivo.

Research Products

• [Journal Article] PTB deficiency causes the loss of adherens junctions in the dorsal telencehalon and leads to lethal (2013)
  • Author(s): 柴嵜孝幸
  • Journal Title: Cerebral Cortex Volume: 一(掲載予定) Issue: 8 Pages: 1-12
  • DOI: 10.1093/cercor/bhs161
  • Peer Reviewed
• [Journal Article] Ras/MAPK Signaling Modulates VEGFR-3 Expression through Ets-Mediated p300 Recruitment and Histone Acetylation on the Vegfr3 Gene in Lymphatic Endothelial Cells. (2012)
  • Author(s): Ichise, T., Yoshida, N., Ichise, H.
  • Journal Title: PLoS One. Volume: 7 Issue: 12 Pages: e51639-e51639
  • DOI: 10.1371/journal.pone.0051639
  • Peer Reviewed
• [Journal Article] Y.Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells. (2012)
  • Author(s): Osada, M., Inoue, O., Ding, G., Shirai, T., Ichise, H., Hirayama, K., Takano, K., Yatomi, Y., Hirashima, M., Fujii, H., Suzuki-Inoue, K., Ozaki
  • Journal Title: The Journal of biological chemistry. Volume: 287 Issue: 26 Pages: 22241-22252
  • DOI: 10.1074/jbc.m111.329987
  • Peer Reviewed
• [Journal Article] Polypyrimidine tract-binding protein regulates the cell cycle through IRES-dependent translation of CDK11(p58) in mouse embryonic stem cells (2011)
  • Author(s): Ohno S, Shibayama M, Sato M, Tokunaga A, Yoshida N
  • Journal Title: Cell Cycle Volume: 10(21) Issue: 21 Pages: 3706-3713
  • DOI: 10.4161/cc.10.21.17903
  • Peer Reviewed
• [Journal Article] N.Fbxl10/Kdm2b deficiency accelerates neural progenitor cell death and leads to exencephaly. (2011)
  • Author(s): Fukuda, T., Tokunaga, A., Sakamoto, R., Yoshida
  • Journal Title: Molecular and cellular neurosciences. Volume: 46 Issue: 3 Pages: 614-624
  • DOI: 10.1016/j.mcn.2011.01.001
  • Peer Reviewed
• [Journal Article] N., Miki, H.Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive Dishevelled protein. (2010)
  • Author(s): Funato, Y., Terabayashi, T., Sakamoto, R., Okuzaki, D., Ichise, H., Nojima, H.,Yoshida
  • Journal Title: Current biology. Volume: 20 Issue: 21 Pages: 1945-1952
  • DOI: 10.1016/j.cub.2010.09.065
• [Journal Article] Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of flightless-I to myeloid differentiation primary response gene (88). (2010)
  • Author(s): Hayashi, T., Funato, Y., Terabayashi, T., Morinaka, A., Sakamoto, R., Ichise, H., Fukuda, H., Yoshida, N., Miki, H.
  • Journal Title: The Journal of biological chemistry. Volume: 285 Issue: 24 Pages: 18586-18593
  • DOI: 10.1074/jbc.m110.106468
• [Journal Article] リンパ管新生におけるRasの役割 (2010)
  • Author(s): 市瀬 広武
  • Journal Title: リンパ学 Volume: 33 Pages: 102-106
  • URL: http://mol.medicalonline.jp/archive/search?jo=ed6lymph&ye=2010&vo=33&issue=2
• [Journal Article] Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of in active dishevelled protein. (2010)
  • Author(s): Funato Y, Terabayashi T, Sakamoto R, Okuzaki D, Ichise H, Nojima H, Yoshida N, Miki H.
  • Journal Title: Current Biology Volume: 20 Pages: 1945-1952
  • Peer Reviewed
• [Presentation] 先進的医学研究のための遺伝子改変動物研究コンソーシアムの概要 (2012)
  • Author(s): 吉田 進昭
  • Organizer: 第85回日本生化学会大会フォーラム「わが国における遺伝子改変動物研究コンソーシアムの取組みと現状」
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2012-12-16
• [Presentation] A Modulatory Role of Ras in VEGFR-3 Signalling during Normal and Pathological Lymphangiogenesis" (2011)
  • Author(s): Hirotake Ichise
  • Organizer: 第32回日本炎症・再生医学会シンポジウム "Tumor Microenvironment and Inflammation"
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-06-02
• [Presentation] A Modulatory Role of Ras in VEGFR-3 Signalling during Normal and Pathological Lymphangiogenesis (2011)
  • Author(s): Hirotake Ichise
  • Organizer: 第32回日本炎症・再生医学会
  • Place of Presentation: 国立京都国際会館(京都)(招待講演)
  • Year and Date: 2011-06-02
• [Presentation] リンパ管新生における Ras の役割 (2010)
  • Author(s): 市瀬 広武
  • Organizer: 第34回日本リンパ学会総会
  • Place of Presentation: 東京大学山上会館(東京)
  • Year and Date: 2010-06-26
• [Presentation] Role of Ras in lymphangiogenesis"Gordon Research Conference 2010, (2010)
  • Author(s): Hirotake Ichise
  • Organizer: Molecular Mechanisms in Lymphatic Function & Disease
  • Place of Presentation: Lucca (Barga), Italy(招待講演)
  • Year and Date: 2010-06-15
• [Book] よくわかるリンパ浮腫のすべて (2011)
  • Author(s): 市瀬 広武(光嶋 勲 編著)
  • Total Pages: 6
  • Publisher: 永井書店
• [Book] よくわかるリンパ浮腫のすべて(分担執筆) (2011)
  • Author(s): 市瀬広武(光嶋勲編著)
  • Total Pages: 6
  • Publisher: 永井書店