| Project/Area Number |
22500384
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ICHISE Taeko 東京大学, 医科学研究所, 助教 (00396863)
YOSHIDA Nobuaki 東京大学, 医科学研究所, 教授 (10250341)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Cre/loxP / FLP/FRT / Dre/rox / 部位特異的組換え酵素 / マウス |
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| Research Abstract |
We identified the transgene integration site of the CGH4 transgenic mice that conditionally express H-Ras and EGFP in a variety of tissues in a Cre/loxP recombination-dependent manner (Ichise et al., 2010), generated new mouse lines using targeting vectors for the locus, and found that the locus is suitable for site-spesific recomibinase-mediated, binary expression of transgenes in an endothelial cell lineage. Targeted transgenesis and binary expression system using the locus in combination with the Rosa26 locus will be valuable for developing multi-step, site-specific recombination system in vivo.
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