Maintenance of bone mineral density in Senescence Accelerated MiceP6 (SAMP6)
| Project/Area Number |
22500684
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TOMITA Masafumi 川崎医科大学, 医学部, 准教授 (50113197)
日高 和夫 川崎医科大学, 医学部, 准教授 (00069064)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 加齢 / 老化 / 老化促進マウス / ビタミンK2 / 骨形態計測 / 水浸拘束ストレス / 骨代謝 / 骨代謝マーカー / 生理活性物質 |
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| Research Abstract |
The P6 strain of senescence-accelerated mice (SAMP6) exhibit features of skeletal aging including reduced bone mineral density (BMD), so that various physiological active agents affecting BMD could be examined. We developed the further bone resorption model using water-immersion restraint stress (WRS). Male SAMP6 were divided into three groups, i.e., control group, WRS (6 h/day, 5 times/wk) group, and WRS + vitamin K2 (VK2) (30 mg/kg s.c.) group. SAMP6 were suffered from this stress between 6 to 10 weeks old. BMD was measured using computed tomography (CT) system for small animals, and fluctuation of bone turnover markers and bone histomorphometrical analysis were also performed.BMD of trabecular bone for WRS groups was significantly lower than that of control group, suggesting that WRS was effective to reduce BMD. However, no significant difference of BMD was observed between WRS group and WRS + VK2 group. In bone turnover markers, urinary excretion of CTX, bone resorption mark，was significantly higher in WRS groups than in control. And urinary excretionof calcium was significantly higher in WRS group than in other groups. On the other hand, bone histomorphometrical analysis indicated that trabeculae of WRS showed sthin and mall shape, but that of WRS + VK2 showed larger and round shape. On the resorption area, large osteoclast was observed in WRS, but the number of osteoclasts was significantly decreased in WRS + VK2. These resultsindicated that vitamin K2 could affect bone turnover.
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Report
(4 results)
Research Products
(9 results)
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[Presentation] Vitamin K2 administration and bone mineral density in Senescence Accelerated Mice P-6 (SAMP6).2012
Author(s)
Katsuyama H, Fushimi S, Yamane K, Hinenoya H, Akiyama Y, Tomita M, Okuyama T, Watanabe Y, Katsuyama M, Ngoc Anh L, Saijoh K.
Organizer
EXPERIMENTAL BIOLOGY '12
Place of Presentation
(San Diego Convention Center, San Diego, CA, USA
Related Report
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[Presentation] Vitamin K2 administration and bone mineral density in Senescence Accelerated Mice P-6 (SAMP6)2012
Author(s)
Hironobu Katsuyama, Shigeko Fushimi, Kunikazu Yamane, Hajime Hinenoya, Yuji Akiyama, Masafumi Tomita, Toshiko Okuyama, Yoko Watanabe, Midori Katsuyama, Le Ngoc Anh, Kiyofumi Saijoh
Organizer
EXPERIMENTAL BIOLOGY
Place of Presentation
San Diego Convention Center (San Diego, USA)
Related Report
