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Study on elucidation of relation between cell-wall related enzymes and physical properties of cooked barley

Research Project

Project/Area Number 22500729
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionNiigata University

Principal Investigator

OHTSUBO Ken-ichi  新潟大学, 自然科学系, 教授 (80353960)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords食品 / 植物 / 酵素 / 物性 / 食品科学 / 大麦 / セルラーゼ / キシラナーゼ / PCR / DNA解析
Research Abstract

Principal properties, such as amylose, protein and physical properties of cooked barley, were evaluated. Template DNAs for PCR were developed using world-wide barley samples, and primers for cell-wall related enzymes, such as cellulase or xylanase, were developed. Novel primers for main proteins, starch-related enzymes, protein Z, hop-related primers, yeast-related primers, were developed. Multiple regression analyses were carried out, using above-mentioned primers, for taste or foam stability of beer, which led to high multiple regression coefficients.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (8 results)

All 2013 2012 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (3 results) Book (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] A Novel Method for the Preparation of Template DNA for PCR from Beer to Detect Materials and to Develop DNA Markers to Evaluate the Quality of Beer2013

    • Author(s)
      Sumiko Nakamura, RyosukeTsushima, Ken'ichi Ohtsubo
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 4 Pages: 820-831

    • DOI

      10.1271/bbb.120969

    • NAID

      10031170725

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Search for Cell-Wall-Degrading Enzymes of World-Wide Rice Grains by PCR and Their Effects on the Palatability of Rice2012

    • Author(s)
      Sumiko Nakamura, KeisukeMachida, Ken'ichi Ohtsubo
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 9 Pages: 1645-1654

    • DOI

      10.1271/bbb.120147

    • NAID

      130004137954

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] ビールを試料とするPCRによる醸造原料および酵母の判別の試み2012

    • Author(s)
      中村澄子、對馬諒介、大坪研一
    • Organizer
      日本食品科学工学会第59回大会
    • Place of Presentation
      藤女子大学
    • Year and Date
      2012-08-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 稲の多様性とその利用について2011

    • Author(s)
      大坪研一
    • Organizer
      第52回食品新素材研究会
    • Place of Presentation
      糖業会館(東京都)
    • Year and Date
      2011-01-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] ビールを試料とするPCRによる醸造原料および酵母の判別の試み

    • Author(s)
      大坪研一
    • Organizer
      日本食品科学工学会誌
    • Place of Presentation
      藤女子大学
    • Related Report
      2012 Annual Research Report
  • [Book] 月刊食料と安全2011年1月号の「米及び米加工品に関する鼓近の研究事例」 5ページ2011

    • Author(s)
      大坪研一・中村澄子
    • Publisher
      全国瑞穂食糧検査協会
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] ビール原料及び製品の判別方法並びにその方法を利用したビール品質の評価方法2012

    • Inventor(s)
      大坪研一・中村澄子
    • Industrial Property Rights Holder
      新潟大学
    • Filing Date
      2012-09-20
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 工業所有権2012

    • Inventor(s)
      大坪研一・中村澄子
    • Industrial Property Rights Holder
      新潟大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-09-20
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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