Development of a method for the pinpoint detection of methylated DNA
| Project/Area Number |
22501037
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Tumor diagnosis
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| Research Institution | Tokyo University of Technology |
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Principal Investigator |
KATO Teru 東京工科大学, 応用生物学部, 准教授 (00367195)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 遺伝子 / がん / メチル化 / エピジェネティクス / 腫瘍マーカー / メチルシトシン / メチル化DNA / がんマーカー / 遺伝子診断 |
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| Research Abstract |
Methylated DNA plays an important role in the epigenetic gene regulation and thus can be a promising biomarker for cancer diagnosis. Therefore, a simple method for the analysis of methylation ratio of cytosines in target gene is highly required. We herein report a new method for the pinpoint detection of 5-methylcytosine that is based on the selective chemical modification of cytosine at the branching point of DNA three-way junction.
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Report
(4 results)
Research Products
(7 results)
