Relationship between a number of polymorphisms on D-loop region of mitochondrial DNA and risk of drinking and smoking for esophageal cancer

• Project/Area Number: 22501063
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cancer epidemiology and prevention
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: KUMIMOTO Hiroshi 愛知県がんセンター(研究所), 中央実験室, 研究員 (00291170)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ミトコンドリアDNA / 多型 / ミトコンドリア / 遺伝子環境交互作用 / 遺伝子多型 / 分子疫学 / がん / 食道 / 疫学 / 癌

Outline of Final Research Achievements

Using a number of polymorphisms o D-loop region of mitochondrial DNA (mtDNA) as an index of somatic mutations on mtDNA, to analyze risk of drinking and smoking for esophageal cancer, case-control study were planed. Comprehensive analysis of D-loop region of mtDNA was done to find polymorphisms.
After DNA sequencing was done for esophageal cancer patients and non-cancer controls, polymorphisms were identified. We found 7.5 polymorphisms among esophageal cancer patients and 6.8 polymorphisms among non-cancer controls on average, respectively. Mainly the difference between cancer patients and non-cancer controls was base substitution. 5.9 base substitutions were identified among esophageal cancer patients and 5.4 among non-cancer controls.