| Project/Area Number |
22510026
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental impact assessment/Environmental policy
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| Research Institution | University of Toyama |
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Principal Investigator |
TANAKA Daisuke 富山大学, 大学院・理工学研究部(理学), 准教授 (40360804)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Shogo 富山大学, 大学院・理工学研究部(理学), 教授 (60134996)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 陸圏 / 水圏 / 大気圏影響評価 / 大気 / バイオエアロゾル / 細菌 / PCR-DGGE / 富山県 |
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| Research Abstract |
Bacterial community structure was investigated by analyzing air samples collected almost weekly on the roof of the Faculty of Science, University of Toyama, Toyama Prefecture, Japan. PCR-DGGE （Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis) was used to analyze changes in the structure of the bacterial communities in the atmosphere. The DGGE profiles showed significant differences among sampling days. Overall, 106 bands were excised from the DGGE gels and subsequently sequenced. About 80% of sequences were affiliated with the phylaProteobacteria, Firmicutes, and Bacteroidetes, and the most common genus was Pseudomonas. We also investigated bacterial communities in Asian dust-containing snow layers on Mt. Tateyama. Interestingly, we detected Bacillus isolates with identical 16S rDNA sequences （AB500941) over 2 consecutive years, and these results might reflect the long-distance transportation of microorganisms from dust source regions.
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