Experimental determination of haplotypes in radiosensitivity-associated genes

• Project/Area Number: 22510215
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical genome science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: MICHIKAWA Yuichi 独立行政法人放射線医学総合研究所, 緊急被ばく医療研究センター, 主任研究員 (20360688)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ゲノム / 放射線 / 遺伝子 / 核酸 / 1分子計測 / １分子計測（SMD） / 1分子計測(SMD) / 1分子計測ア(SMD)

Research Abstract

In this study, experimental haplotype determination has been conducted using cryopreserved human blood samples. Improvement of technology to remove contaminating small DNA fragments generated by freeze and thaw process was carried out. Four SNPs of the CD44 gene were selected and analysis of 50 frozen human bloods was attempted by this experimental system. Failure of 6 samples indicated that some physical properties of DNA preparation might be affecting suitability for this experimental system. Further improvement should be necessary to overcome this.

Research Products

• [Journal Article] Combination effects of distinct cores in 11q13 amplification region on cervical lymph node metastasis of oral squamous cell carcinoma (2011)
  • Author(s): K.Sugahara, Y.Michikawa
  • Journal Title: International Journal of Oncology Volume: 39 Pages: 761-769
  • DOI: 10.3892/ijo.2011.1094
  • Peer Reviewed
• [Journal Article] Genetic variants ofNPAT-ATM and AURKA are associated withan early adverse reaction in thegastrointestinal tract of patientswith cervical cancer treated withpelvic radiation therapy. (2011)
  • Author(s): Ishikawa A, Suga T, Shoji Y, Kato S,Ohno T, Ishikawa H, Yoshinaga S, OharaK, Ariga H, Nomura K, Shibamoto Y,Ishikawa K, Moritake T, Michikawa Y,Iwakawa M, Imai T.
  • Journal Title: Int JRadiat Oncol Biol Phys. Volume: 81 Issue: 4 Pages: 1144-52
  • DOI: 10.1016/j.ijrobp.2010.09.012
  • Peer Reviewed
• [Journal Article] Dynamics of Delayed p53 Mutations in Mice Given Whole-Body Irradiation at Eight Weeks (2011)
  • Author(s): R.Okazaki, Y.Michikawa, et al
  • Journal Title: International Journal of Radiation Oncology Biology Physics Volume: 79 Issue: 1 Pages: 247-254
  • DOI: 10.1016/j.ijrobp.2010.07.022
  • Peer Reviewed
• [Presentation] Basics of the interaction between the human body and ionizing radiation (2011)
  • Author(s): Yuichi Michikawa
  • Organizer: 2011 UOEH International Symposium "Health of Workers Responding to A Nuclear Crisis"
  • Place of Presentation: ヒルトン福岡シーホーク
  • Year and Date: 2011-11-09