| Project/Area Number |
22510232
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Yamaguchi University |
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Principal Investigator |
MIGITA Taiko 山口大学, 農学部, 教授 (90159161)
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| Co-Investigator(Kenkyū-buntansha) |
OZAKI Shinichi 山口大学, 農学部, 教授 (40280581)
MIGITA Kouto 山口大学, 農学部, 教授 (90116757)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Michihiko 山形大学, 医学部, 准教授 (00135344)
ZHANG Xuhong 山形大学, 医学系研究院, 助教 (10292442)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 酵素活性の微調節 / 酸素消費の速度論解析 / 酸性化環境への適応 / ESI-MSによる逐次反応速度論解析 / ヘムオキシゲナーゼ / 環境応答機構 / 反応調節 / 環境変化 |
|---|
| Research Abstract |
Different biological species, whose biological niches are different, commonly retain heme degradation enzyme, heme oxygenase (HO), where heme is indispensable for proceeding many in vivo reactions. We have prepared HOs from a land mammalian, rat, marine puffer fish, a land plant, soybean, and a freshwater cyanobacterium and compared their heme degradation activity under different reaction conditions. As a result, we have found that the HO from different origin is affected at different reaction stages under respective reaction conditions.
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