Method for Pin-Point Structure Analysis of Proteins by Fluorescence-Detected CD
| Project/Area Number |
22550038
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Organic chemistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
NEHIRA Tatsuo 広島大学, 大学院・総合科学研究科, 助教 (60321692)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAZAKI Takeshi 広島大学, 大学院・総合科学研究科, 教授 (30192397)
ISHIDA Atsuhiko 広島大学, 大学院・総合科学研究科, 准教授 (90212886)
HIRANO Tetsuo 広島大学, 大学院・総合科学研究科, 教授 (50228805)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 構造有機化学 / 蛋白質 / 円二色性 / 蛍光 / タンパク質 / CD |
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| Research Abstract |
Fluorescence-detected circular dichroism (FDCD) was applied to model proteins for the first time with the device that we have recently developed. Using metmyoglobin, a tryptophan-containing protein that gets denatured separately atsecondary and tertiary structure levels depending on the pH changes, it was unveiled that FDCD mainly observes the change of tertiary structure. Using a fluorescently-labeledcalmodulin, which alters its structure by the existence of calcium ions and/or binding peptides, it was demonstrated that FDCD can selectively observe the structure changes of the target protein.
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Report
(4 results)
Research Products
(5 results)
