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Development of electrochemical sensor using biocomposite material as the reaction field of binding between biomolecules

Research Project

Project/Area Number 22550078
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionMaebashi Institute of Technology

Principal Investigator

SUGAWARA Kazuharu  前橋工科大学, 工学部, 教授 (30271753)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords生体高分子 / 複合材料 / カーボン / タンパク質-タンパク質相互作用 / 磁性ビーズ / オボアルブミン / コンカナバリンA / Phos-tag / ガリウム(III)錯体 / キチン / キトサン / アビジン-ビオチン間結合 / ラベル化 / 乳酸菌 / 生体分子 / 生体材料 / コラーゲン / カーボンファイバー / ボルタンメトリー
Research Abstract

In the present study, biopolymer-carbon and biopolymer- biopolymer composite materials were constructed in order to enhance the functionalization of the biopolymeric materials. First, the electrochemical monitoring of biomolecule produced from bacteria enclosed in the material and the surface modification of the composite material covered with a functional protein were performed. Second, the biomolecules wereimmobilized to the material that was considered as the reaction field of the molecular interaction. Furthermore, the characteristic of each material was analyzed, and the electrochemical sensing systems of the biomolecules were developed.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (38 results)

All 2012 2011 2010

All Journal Article (17 results) (of which Peer Reviewed: 15 results) Presentation (21 results)

  • [Journal Article] Electrochemical Genotoxicity Assay Based on a SOS/umu Test Using Hydrodynamic Voltammetry in a Droplet2012

    • Author(s)
      Kuramitz, H.; Sazawa, K.; Nanayama, Y.; Hata, N.; Taguchi, S.; Sugawara, K.;Fukushima, M.
    • Journal Title

      Sensors

      Volume: 12 Pages: 17414-17432

    • NAID

      120005158961

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical assay of concanavalin A-ovalbumin binding on magnetic beads2012

    • Author(s)
      Sugawara, K.; Yugami, A.; Kadoya T.;Kuramitz, H.; Hosaka, K.
    • Journal Title

      Analyst

      Volume: 137 Pages: 3781-3786

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Voltammetric sensing of phosphoproteins using a gallium(III) acetylacetonatemodified carbon paste electrode2012

    • Author(s)
      Sugawara, K.; Yugami, A.; Kadoya, T.
    • Journal Title

      Anal.Sci.

      Volume: 28 Pages: 251-255

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiplexed Assay for Proteins Based on SequestrationElectrochemistry Using the Protein Binding Electroactive Magnetic Microbeads2012

    • Author(s)
      Kuramitz, H. ; Mawatari, Y. ; Ikeuchi, M. ; Kutomi, O. ; Hata, N. ; Taguchi, S. ; Sugawara, K.
    • Journal Title

      Anal. Sci

      Volume: 23 Pages: 77-82

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical assay of concanavalin A-ovalbumin binding on magnetic beads2012

    • Author(s)
      Sugawara, K., Yugami, A., Kadoya T., Kuramitz, H., Hosaka, K.
    • Journal Title

      Analyst

      Volume: Vol. 137 Issue: 16 Pages: 3781-3786

    • DOI

      10.1039/c2an35667h

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical mutagenicity assay based on SOS/umu test using hydrodynamic voltammetry in a droplet.2012

    • Author(s)
      H. Kuramitz, M. Fukushima
    • Journal Title

      Sensors

      Volume: 12 Issue: 12 Pages: 17414-17432

    • DOI

      10.3390/s121217414

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Voltammetric Sensing of Phosphoproteins Using a Gallium(III) Acetylacetonate-Modified Carbon Paste Electrode2012

    • Author(s)
      Sugawara, K.; Yugami, A.; Kadoya, T.; Hosaka, K.
    • Journal Title

      Analytical Sciences

      Volume: 28 Issue: 3 Pages: 251-251

    • DOI

      10.2116/analsci.28.251

    • NAID

      130004442122

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiplexed Assay for Proteins Based on Sequestration Electrochemistry Using the Protein Binding Electroactive Magnetic Microbeads2012

    • Author(s)
      Kuramitz, H.; Mawatari, Y.; Ikeuchi, M.; Kutomi, O.; Hata, N.; Taguchi, S.; Sugawara, K.
    • Journal Title

      Analytical Sciences

      Volume: 28 Issue: 1 Pages: 77

    • DOI

      10.2116/analsci.28.77

    • NAID

      130004827183

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemically monitoring the binding of concanavalin A and ovalbumin2011

    • Author(s)
      Sugawara, K.; Yugami, A.; Kadoya, T.; Hosaka, K.
    • Journal Title

      Talanta

      Volume: 85 Pages: 425-429

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] キチン-キトサン膜で被覆された電極を用いるタンパク質-リガンド間結合の電気化学的評価2011

    • Author(s)
      菅原一晴
    • Journal Title

      分析化学

      Volume: 60 Pages: 353-356

    • NAID

      10028107116

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Binding assay for cholera toxin based on sequestration electrochemistry using lactose labeled with an electroactivecompound2011

    • Author(s)
      Kuramitz, H.; Miyagaki, S.; Ueno, E.; Hata,N.; Taguchi S.; Sugawara, K.
    • Journal Title

      Analyst

      Volume: 136 Pages: 2327-2378

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] ELISA によるグルコース/グリコアルブミンアッセイ法の開発2011

    • Author(s)
      菅原一晴、 竹部拓也
    • Journal Title

      前橋工科大学研究紀要

      Volume: 14 Pages: 93-96

    • NAID

      40018877543

    • Related Report
      2012 Final Research Report
  • [Journal Article] Binding assay for cholera toxin based on sequestration electrochemistry using lactose labeled with an electroactive compound2011

    • Author(s)
      Kuramitz, H.; Miyagaki, S.; Ueno, E.; Hata, N.; Taguchi, S.; Sugawara, K.
    • Journal Title

      Analyst

      Volume: Vol.136 Issue: 11 Pages: 2373-2378

    • DOI

      10.1039/c1an15100b

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Evaluation of Protein-Ligand Binding Using an Electrode Covered with Chitin-Chitosan Film2011

    • Author(s)
      菅原一晴、由上麻子
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 60 Issue: 4 Pages: 353-356

    • DOI

      10.2116/bunsekikagaku.60.353

    • NAID

      10028107116

    • ISSN
      0525-1931
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ELISAによるグルコース/グリコアルブミンアッセイ法の開発2011

    • Author(s)
      菅原一晴、竹部拓也
    • Journal Title

      前橋工科大学研究紀要

      Volume: 14巻 Pages: 93-96

    • NAID

      40018877543

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Voltammetric Detection of Biological Molecules Using Chopped Carbon Fiber2010

    • Author(s)
      Sugawara, K.; Yugami, A.; Kojima, A.
    • Journal Title

      Ana. Sci.

      Volume: 26 Pages: 1059-1063

    • NAID

      10026785664

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Voltammetric Detection of Biological Molecules Using Chopped Carbon Fiber2010

    • Author(s)
      Sugawara, K., ; Yugami, A., : Kojima, A.
    • Journal Title

      Analytical Sciences

      Volume: VOL.26 Pages: 1059-1064

    • NAID

      10026785664

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] マンノースとチオニンを修飾した金ナノ粒子被覆磁性マイクロ粒子を用いる大腸菌の電気化学アッセイの開発2012

    • Author(s)
      今井健一郎、倉光英樹、波多宣子、田口茂、菅原一晴
    • Organizer
      日本化学会近畿支部 平成24年度研究発表会
    • Place of Presentation
      福井、福井大学文京キャンパス
    • Year and Date
      2012-11-17
    • Related Report
      2012 Final Research Report
  • [Presentation] マンノースとチオニンを修飾した金ナノ粒子被覆磁性マイクロ粒子を用いる大腸菌の電気化学アッセイの開発2012

    • Author(s)
      今井健一郎,倉光英樹,波多宣子,田口茂,菅原一晴
    • Organizer
      日本化学会近畿支部 平成24年度研究発表会
    • Place of Presentation
      福井大学文京キャンパス(福井県)
    • Year and Date
      2012-11-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] タンパク質認識能を有する電極活性磁性マイクロ粒子を用いた大腸菌アッセイの開発2012

    • Author(s)
      馬渡暢子、倉光英樹、波多宣子、田口茂、菅原一晴
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      石川、金沢大学 角間キャンパス
    • Year and Date
      2012-09-19
    • Related Report
      2012 Final Research Report
  • [Presentation] 標識ペプチド-オボアルブミン間相互作用のボルタンメトリー的評価2012

    • Author(s)
      菅原一晴、 門屋利彦、 倉光英樹、由上麻子、田中俊逸
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      石川、金沢大学 角間キャンパス
    • Year and Date
      2012-09-19
    • Related Report
      2012 Final Research Report
  • [Presentation] 電気化学的変異原性試験法を用いた土壌燃焼物の変異原性評価2012

    • Author(s)
      佐澤和人、倉光英樹、波多宣子、田口茂、菅原一晴
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      石川、金沢大学 角間キャンパス
    • Year and Date
      2012-09-19
    • Related Report
      2012 Final Research Report
  • [Presentation] 電気化学的変異原性試験法を用いた土壌燃焼物の変異原性評価2012

    • Author(s)
      佐澤和人、倉光英樹、波多宣子、田口茂、菅原一晴
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学角間キャンパス(石川県)
    • Year and Date
      2012-09-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] 標識ペプチドーオボアルブミン間相互作用のボルタンメトリー的評価2012

    • Author(s)
      菅原一晴、門屋利彦、倉光英樹、由上麻子、田中俊逸
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学角間キャンパス(石川県)
    • Year and Date
      2012-09-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] タンパク質認識能を有する電極活性磁性マイクロ粒子を用いた大腸菌アッセイの開発2012

    • Author(s)
      馬渡暢子、倉光英樹、波多宣子、田口茂、菅原一晴
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢大学角間キャンパス(石川県)
    • Year and Date
      2012-09-19
    • Related Report
      2012 Annual Research Report
  • [Presentation] 糖質部位をもつ非イオン性界面活性剤を利用したレクチンの電気化学的センシング2012

    • Author(s)
      菅原一晴、倉光英樹、由上麻子
    • Organizer
      第72回分析化学討論会
    • Place of Presentation
      鹿児島、鹿児島大学郡元キャンパス
    • Year and Date
      2012-05-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 糖質部位をもつ非イオン性界面活性剤を利用したレクチンの電気化学的センシング2012

    • Author(s)
      菅原一晴、倉光英樹、由上麻子
    • Organizer
      第72回分析化学討論会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県)
    • Year and Date
      2012-05-20
    • Related Report
      2012 Annual Research Report
  • [Presentation] ガリウム錯体修飾カーボンペースト電極を用いたリンタンパク質の電気化学的センシング2011

    • Author(s)
      菅原一晴、由上麻子、倉光英樹、門屋利彦
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋、名古屋大学
    • Year and Date
      2011-09-15
    • Related Report
      2012 Final Research Report
  • [Presentation] コンカナバリン A-オバルミン間結合の電気化学的モニタリング2011

    • Author(s)
      菅原一晴、 由上麻子、倉光英樹、 保坂公平
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台、東北大学
    • Year and Date
      2011-09-15
    • Related Report
      2012 Final Research Report
  • [Presentation] 分子認識能を導入した磁性マイクロ粒子を用いるタンパク質の電気化学的アッセイの開発2011

    • Author(s)
      池内麻り愛、倉光英樹、菅原一晴、波多宣子、田口 茂
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台、東北大学
    • Year and Date
      2011-09-15
    • Related Report
      2012 Final Research Report
  • [Presentation] ガリウム錯体修飾カーボンペースト電極を用いたリンタンパク質の電気化学的センシング2011

    • Author(s)
      菅原一晴・由上麻子・倉光英樹・門屋利彦
    • Organizer
      日本分析化学会第60年会
    • Place of Presentation
      名古屋大学(名古屋)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] One-step Binding Assay by Magnetic Microbead-based Sequestration Electrochemistry for Cholera Toxin2011

    • Author(s)
      Ikeuchi, M.; Kuramitz, H.; Hata, N.; Taguchi, S.; Sugawara, K.
    • Organizer
      The 62nd ISE Annual Meeting in Niigata
    • Place of Presentation
      Niigata Convention Center
    • Year and Date
      2011-09-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Electrochemical assay of Concanavalin A-Ovalbumin binding using magnetic beads2011

    • Author(s)
      Sugawara, K.; Yugami, A.; Kuramitz, H.; Hosaka, K.
    • Organizer
      IUPAC International Congress on Analytical Sciences 2011
    • Place of Presentation
      Kyoto International Conference Center
    • Year and Date
      2011-05-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Electrochemical evaluation of biomolecular interaction using an electrode covered with a biomaterial film2010

    • Author(s)
      Sugawara, K., Yugami, A, Tanaka, S.
    • Organizer
      2010 International Chemical Congress of Pacific Basin Sacieties
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] コンカナバリンA-オバルミン間結合の電気化学的モニタリング2010

    • Author(s)
      菅原一晴・由上麻子・倉光英樹・保坂公平
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台、東北大学
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 分子認識能を導入した磁性マイクロ粒子を用いるタンパク質の電気化学的アッセイの開発2010

    • Author(s)
      池内麻り愛・倉光英樹・菅原一晴・波多宣子・田口茂
    • Organizer
      日本分析化学会第59年会
    • Place of Presentation
      仙台、東北大学
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] The protein sensing by sequestration electrochemistry and its applications2010

    • Author(s)
      Kuramitz, H., Sugawara, K.
    • Organizer
      3rd Penang International Conference for Young Chemists
    • Place of Presentation
      Copthorne Orchid Hotel Penang, Malaysia
    • Year and Date
      2010-06-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 炭素繊維チョップトファイバーを用いた電気化学的センサの試作2010

    • Author(s)
      菅原一晴、由上麻子、小島昭
    • Organizer
      第71回分析化学討論会
    • Place of Presentation
      島根、島根大学
    • Year and Date
      2010-05-16
    • Related Report
      2012 Final Research Report 2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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