Study on the Weak Interaction Regulating the Electronic Structure of Blue Copper Protein

• Project/Area Number: 22550145
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Ibaraki University
• Principal Investigator: KOHZUMA Takamitsu 茨城大学, 理工学研究科, 教授 (50215183)
• Co-Investigator(Kenkyū-buntansha): UNNO Masaki 茨城大学, フロンティア応用原子科学研究センター, 准教授 (10359549)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ブルー銅蛋白質 / 弱い相互作用 / シュウドアズリン / X線結晶構造解析 / X線吸収スペクトル / ブルー銅タンパク質 / ラマンスペクトル

Research Abstract

The Met16X pseudoazurin mutants were constructed, and spectroscopic and electrochemical properties of the mutant proteins were investigated. The electronic absorption and EPR spectra of Met16X variant proteins demonstrated the axial/rhombic components are rationally changeable by the characrer of the side chaing group of respective amino acids. Moreover the structure and function of Met16His can be modulated as a function of pH by the protonation/deprotonation of the newly introduced histidine moiety.

Research Products

• [Journal Article] X-ray Absorption Spectroscopic and EXAFS Studies of Unique Plastocyanin from a Fern, Dryopteris crassirhizoma (2013)
  • Author(s): Hiromi Togashi, Junko Yano, Vittal K. Yachandra, Takamitsu Kohzuma
  • Journal Title: Chemistry Letter Volume: 42 Issue: 1 Pages: 89-90
  • DOI: 10.1246/cl.2013.89
  • NAID: 130004426730
  • Peer Reviewed
• [Journal Article] Direct electron transfer from pseudoazurin to nitrous oxide reductase in catalytic N_2O reduction (2012)
  • Author(s): K. Fujita, M. H.-Fujita, D. E. Brown, Y. Obara, F. Ijima, T. Kohzuma, D. M. Dooley
  • Journal Title: J. Inorg. Biochem Volume: 115 Pages: 163-173
  • Peer Reviewed
• [Journal Article] Direct electron transfer from pseudoazurin to nitrous oxide reductase in catalytic N2O reduction (2012)
  • Author(s): Koyu Fujita
  • Journal Title: Journal of Inorganic Biochemistry Volume: 115 Pages: 163-173
  • DOI: 10.1016/j.jinorgbio.2012.07.013
  • Peer Reviewed
• [Journal Article] Theoretical studies on model reaction pathways of prostaglandinH2 isomerization to prostaglandin D2/E2 (2011)
  • Author(s): Naoto Yamaguchi, Tatsuya Naiki, Takamitsu Kohzuma, Toshikazu Takada, Fumihiko Sakata, Seiji Mori
  • Journal Title: Theor Chem Acc Volume: 128 Issue: 2 Pages: 191-206
  • DOI: 10.1007/s00214-010-0814-7
  • Peer Reviewed
• [Journal Article] Theoretical Studies on Model Reaction Pathways of Prostaglandin H_2 Isomerization to Prostaglandin D_2/E_2 (2011)
  • Author(s): Naoto Yamaguchi
  • Journal Title: Theoretical Chemistry Accounts Volume: 128 Pages: 191-206
  • Peer Reviewed
• [Journal Article] UV Resonance Raman Studies on the Activation Mechanism of Human Hematopoietic Prostaglandin D_2 Synthase by a Divalent Cation (2010)
  • Author(s): Yoshiko Uchida, Yoshihiro Urade, Seiji Mori, and Takamitsu Kohzuma
  • Journal Title: Mg^<2+> J. Bioinorg. Chem Volume: 104 Issue: 3 Pages: 331-340
  • DOI: 10.1016/j.jinorgbio.2009.12.003
  • Peer Reviewed
• [Journal Article] Czernuszewicz, Non-covalent interactions in blue copper protein probed by Met16 mutation and electronic and resonance Raman spectroscopy of Achromobacter cycloclastes pseudoazurin (2010)
  • Author(s): Marzena B. Fitzpatrick, Yuji Obara, Koyu Fujita, Doreen E. Brown, David M. Dooley, Takamitsu Kohzuma, Roman S
  • Journal Title: J. Bioinorg. Chem Volume: 104 Issue: 3 Pages: 250-260
  • DOI: 10.1016/j.jinorgbio.2009.11.004
  • Peer Reviewed
• [Presentation] Alcaligenes xylosoxidans由来シトクロムc’の全体構造及び電子状態のpH依存性と相関 (2013)
  • Author(s): 高階明子, Michael Tiedemann, 海野昌喜, Martin Stillman, （高妻孝光）
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学
• [Presentation] ブルー銅タンパク質シュウドアズリンH6V変異体の構造と性質 (2013)
  • Author(s): 坂入剛、海野昌樹、（高妻孝光）
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける弱い相互作用の効果 (2012)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 横浜
  • Year and Date: 2012-03-25
• [Presentation] ブルー銅タンパク質シュウドアズリンThr36Lys変異体の構造と性質 (2012)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 横浜
  • Year and Date: 2012-03-25
• [Presentation] Weak Interaction Effects on the Protein Stability of Blue Copper Protein, Pseudoazurin from Achromobacter cycloclastes IAM 1013 (2012)
  • Author(s): T. Yamaguchi, Y, Nihei, S. Asamura, D. Sutherland, M. Stillman, T. Kohzuma
  • Organizer: 11th European Biological Inorganic Chemistry Conference
  • Place of Presentation: Granada(スペイン)
• [Presentation] Effect of "Proton Switch" Induced by the Newly Introduced Uncoordinated Histidine in the Vicinity of the Blue Copper Active Site: High Resolution X-ray Crystallographic, Spectroscopic, and Electrochemical Studies of Met16His Pseudoazurin (2012)
  • Author(s): Takamitsu Kohzuma, Sayaka Asamura, Yuko Nihei,and Maski Unno
  • Organizer: 金属の関連す生体関連反応シンポジウム
  • Place of Presentation: 金沢大学
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける弱い相互作用の効果 (2012)
  • Author(s): 山口峻英, 仁平裕子, 松儀可奈子、Duncan Sutherland、矢野淳子、Vittal Yachandra、Martin Stillman、高妻孝光
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶應義塾大学日吉キャンパス
• [Presentation] シトクロームc’のダイナミクスに関する検討 (2012)
  • Author(s): 高階明子, Michael Tiedemann, 海野昌喜, Martin Stillman, （高妻孝光）
  • Organizer: 第22回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 金沢大学
• [Presentation] Dynamic and Static Structures and Properties of Cytochrome c’ from Alcaligenes xylosoxidans NCIMB 11015 (2012)
  • Author(s): Akiko Takashina, Michael Tiedemann, Martin Stillman, (Takamitsu Kohzuma)
  • Organizer: 11th European Biological Inorganic Chemistry Conference
  • Place of Presentation: Granada, Spain
• [Presentation] ヘムタンパク質シトクロームc’の特異的pH依存性 (2012)
  • Author(s): 高階明子, Michael Tiedemann, 海野昌喜, Martin Stillman, （高妻孝光）
  • Organizer: 第23回日本化学会関東支部茨城地区研究交流会
  • Place of Presentation: 日立市シビックセンター
• [Presentation] Alcaligenes xylosoxidas由来シトクロームc'の分光学的性質及びpH依存性 (2012)
  • Author(s): 高階明子, Michael Tiedemann, 海野昌喜, Martin Stillman, （高妻孝光）
  • Organizer: 第85回日本生化学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Unique Electronic Structure and Dynamics of Cytochrome c' from Alcaligenes xylosoxidans (2012)
  • Author(s): 高階明子, Michael Tiedemann, 海野昌喜, Martin Stillman, （高妻孝光）
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] Weak Interaction Effects on the Protein Stability of Blue Copper Protein, Pseudoazurin from Achromobacter cycloclastes IAM 1013 (2012)
  • Author(s): T. Yamaguchi, Y, Nihei, S. Asamura, D. Sutherland, M. Stillman, (T. Kohzuma)
  • Organizer: 11th European Biological Inorganic Chemistry Conference
  • Place of Presentation: Granada, Spain
• [Presentation] Structure and Properties of a Blue Copper Protein, Pseudoazurin His6Val Mutant (2012)
  • Author(s): 坂入剛、松儀可奈子、海野昌樹、（高妻孝光）
  • Organizer: 第22回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 金沢大学
• [Presentation] Two steps alkaline transition of stellacyanin from lacquer tree sap of Okukuji Urushi (2012)
  • Author(s): 室矢知徳、高橋里佳、寺門秀人、神長正則、Michael Tiedemann、Martin j. Stillman、 Roman S. Czernuszewicz、（高妻孝光）
  • Organizer: 第22回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 金沢大学
• [Presentation] Switch Effect Induced by the Newly Introduced Uncoordinated Histidine in the Vicinity of the Blue Copper Active Site: High Resolution X-ray Crystallographic, Spectroscopic, and Electrochemical Studies of Met16His Pseudoazurin (2012)
  • Author(s): (Takamitsu Kohzuma), Sayaka Asamura, Yuko Nihei, Masaki Unno
  • Organizer: 11th European Biological Inorganic Chemistry Conference
  • Place of Presentation: Granada, Spain
• [Presentation] 福島第一原子力発電所事故に伴う放射性物質放出と環境・食品への影響, リスクコミュニケーション (2012)
  • Author(s): （高妻孝光）
  • Organizer: 日本分析化学会第６１年会
  • Place of Presentation: 金沢大学
  • Invited
• [Presentation] XAS Studies on Electronic Structures of Metal Sites in Blue Copper Protein, fern Dryopteris chrassirhizoma (2011)
  • Author(s): 高妻孝光
  • Organizer: International Workshop on Improving Data Quality and Quantity for XAFS Experiments
  • Place of Presentation: KEK, Tsukuba
  • Year and Date: 2011-12-09
• [Presentation] Week Interaction Effect Study of Blue Copper Protein with XAS (2011)
  • Author(s): 高妻孝光
  • Organizer: International Workshop on Improving Data Quality and Quantity for XAFS Experiments
  • Place of Presentation: KEK, Tsukuba
  • Year and Date: 2011-12-09
• [Presentation] オシダ由来プラストシアニンのX線吸収スペクトル (2011)
  • Author(s): 高妻孝光
  • Organizer: 第22回日本化学会茨城地区研究交流会
  • Place of Presentation: 東海村
  • Year and Date: 2011-11-04
• [Presentation] 分光学および精密結晶構造解析によるヘムタンパク質シトクロームc'のリガンド結合機構と電子状態との相関 (2011)
  • Author(s): 高妻孝光
  • Organizer: 第22回日本化学会茨城地区研究交流会
  • Place of Presentation: 東海村
  • Year and Date: 2011-11-04
• [Presentation] ブルー銅タンパク質シュウドアズリンの活性中心構造のダイナミクスと電子状態 (2011)
  • Author(s): 高妻孝光
  • Organizer: 第22回日本化学会茨城地区研究交流会
  • Place of Presentation: 東海村
  • Year and Date: 2011-11-04
• [Presentation] 炎症発現系におけるプロスタグランジンD_2合成酵素における阻害反応機構の検討 (2011)
  • Author(s): 高妻孝光
  • Organizer: 第22回日本化学会茨城地区研究交流会
  • Place of Presentation: 東海村
  • Year and Date: 2011-11-04
• [Presentation] 奥久慈産漆中タンパク質Stellacyaninのアルカリ構造転移評価 (2011)
  • Author(s): 高妻孝光
  • Organizer: 第22回日本化学会茨城地区研究交流会
  • Place of Presentation: 東海村
  • Year and Date: 2011-11-04
• [Presentation] Precise Crystal Structure and Spectroscopic Analyses of a Heme Protein, Cytochrome c' (2011)
  • Author(s): 高妻孝光
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
  • Year and Date: 2011-09-16
• [Presentation] Effect of the non-covalent weak interaction on the electronic structure of blue copper protein (2011)
  • Author(s): 高妻孝光
  • Organizer: 3^<rd> Georgian Bay International Conference of Bioinorganic Chemistry
  • Place of Presentation: Parry Sound, Canada(招待講演)
  • Year and Date: 2011-06-02
• [Presentation] Additivity of Weak Interaction Effect in a Blue Copper Protein, Met16His/Thr36Lys Pseudoazurin (2011)
  • Author(s): 高妻孝光
  • Organizer: 3^<rd> Georgian Bay International Conference of Bioinorganic Chemistry
  • Place of Presentation: Parry Sound, Canada
  • Year and Date: 2011-06-02
• [Presentation] Alkaline Structural Transition of Laccase from Rhus vernicifera of Okukuji Area, Ibaraki Prefecture, Japan (2011)
  • Author(s): 高妻孝光
  • Organizer: 3^<rd> Georgian Bay International Conference of Bioinorganic Chemistry
  • Place of Presentation: Parry Sound, Canada
  • Year and Date: 2011-06-02
• [Presentation] X-ray Absorption Spectroscopic Studies of Blue Copper Proteins, Fern Plastocyanin and Pseudoazurin (2011)
  • Author(s): 高妻孝光
  • Organizer: 3^<rd> Georgian Bay International Conference of Bioinorganic Chemistry
  • Place of Presentation: Parry Sound, Canada
  • Year and Date: 2011-06-02
• [Presentation] Atomic Resolution X-ray crystallographic Analyses and Spectroscopic Studies of a Cytochrome c' from Alcaligenes xylosoxidans (2011)
  • Author(s): 高妻孝光
  • Organizer: 3^<rd> Georgian Bay International Conference of Bioinorganic Chemistry
  • Place of Presentation: Parry Sound, Canada
  • Year and Date: 2011-06-02
• [Presentation] マルチ銅タンパク質ラッカーゼの構造転移 (2011)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-29
• [Presentation] オシダ由来プラストシアニンのEXAFSスペクトル (2011)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-29
• [Presentation] Met16Hia/Thr36Lyaシュウドアズリンの構造と性質 (2011)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] 原子レベル分解能でのAlcaligenes xylosoxidans由来シトクロムげの分子構造と分光学的性質 (2011)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] シュウドアズリンM16X変異体と亜硝酸還元酵素との電子移動反応 (2011)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] ブルー銅タンパク質シュウドアズリンの電子状態に影響を与えるタンパク質内相互作用 (2011)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] 銅型亜硝酸還元酵素の電子伝達機構の解明 (2011)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] プロスタグランジンD_2酵素の分光学的性質 (2011)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2011-03-26
• [Presentation] Structure and Spectroscopic Properties of Non-covalent Interaction of Pseudoazurin (2011)
  • Author(s): 高妻孝光
  • Organizer: Gordon Research Conference, Metals in Biology
  • Place of Presentation: Four Points Sheraton, Ventura(ベンチュラ、米国)
  • Year and Date: 2011-01-30
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける弱い相互作用の加成性 (2011)
  • Author(s): 山口峻英、仁平裕子、浅村紗矢香、Duncan Sutherland, Martin Stillman, 高妻孝光
  • Organizer: 第22回日本化学会茨城地区研究交流会
  • Place of Presentation: 東海村
• [Presentation] Effect of the non-covalent weak interaction on the electronic structure of blue copper protein (2011)
  • Author(s): T. Kohzuma, Y. Nihei, S. Asamura, M. Unno, Y. Obara, T. Yamaguchi, K. Matsugi, R. S. Czernuszewicz , D. Sutherland, M. Stillman, and R. K. Szilagyi
  • Organizer: Parry Sound
  • Place of Presentation: Canda
• [Presentation] Additivity of Weak Interaction Effect in a Blue Copper Protein, Met16His/Thr36Lys Pseudoauzrin (2011)
  • Author(s): T. Yamaguchi, Y. Nihei, S. Asamura, T. Kohzuma
  • Organizer: 3rd Georgian Bay International Conference on Bioinorganic Chemistry
  • Place of Presentation: Parry Sound(カナダ)
• [Presentation] Alkaline Structural Transition of Laccase from Rhus vernicifera of Okukuji Area, Ibaraki Prefecture, Japan (2011)
  • Author(s): Tomonori Muroya, Rika Takahashi, Hideto Terakado, Masanori Kaminaga and Takamitsu Kohzuma
  • Organizer: 3rd Georgian Bay International Conference on Bioinorganic Chemistry
  • Place of Presentation: Parry Sound(カナダ)
• [Presentation] Atomic Resolution X-ray crystallographic Analyses and Spectroscopic Studies of a Cytochrome (2011)
  • Author(s): Akiko Takashina, Masaki Unno and Takamitsu Kohzuma
  • Organizer: c' from Alcaligenes xylosoxidans
  • Place of Presentation: Parry Sound(カナダ)
• [Presentation] X-ray Absorption Spectroscopic Studies of Blue Copper Proteins, Fern Plastocyanin and Pseudoazurin (2011)
  • Author(s): Hiromi Togashi, Sayaka Asamura, Junko Yano, Vittal Yachandra, Fuminori Yoshizaki, and Takamitsu Kohzuma
  • Organizer: 3rd Georgian Bay International Conference on Bioinorganic Chemistry
  • Place of Presentation: Parry Sound(カナダ)
• [Presentation] two steps alkaline transition of stellacyanin revealed byresonance Raman and electrochemistry (2010)
  • Author(s): 高妻孝光
  • Organizer: 2010年環太平洋国際化学会議
  • Place of Presentation: Hawaii Convention Center(ホノルル、米国)
  • Year and Date: 2010-12-17
• [Presentation] Effect of Weak Interaction on the Electronic Structure and Electrochemical Properties of Pseudoazurin Metl6X mutants (2010)
  • Author(s): 高妻孝光
  • Organizer: 2010年環太平洋国際化学会議ト
  • Place of Presentation: Hawau Coventon Center(ホノルル、米国)
  • Year and Date: 2010-12-17
• [Presentation] X-ray crystal structure analyses of a blue copper protein, pseudoazurin from Achromobacter cycloclestes IAM1013 and its mutant proteins (2010)
  • Author(s): 高妻孝光
  • Organizer: 2010年環太平洋国際化学会議
  • Place of Presentation: Hawaii Convention Center(ホノルル、米国)
  • Year and Date: 2010-12-17
• [Presentation] Precise X-ray Crystallographic Analysis of a Cytochrome c from Alcalygenes xylosoxidans NCIMB 11015 (2010)
  • Author(s): 高妻孝光
  • Organizer: 2010年環太平洋国際化学会議
  • Place of Presentation: Hawaii Convention Center(ホノルル、米国)
  • Year and Date: 2010-12-17
• [Presentation] Structure and functions of non-covalent weak interaction in a blue copper protein, paeudoazurin (2010)
  • Author(s): 高妻孝光
  • Organizer: 2010年環太平洋国際化学会議
  • Place of Presentation: Hawaii Convention Center(ホノルル、米国)
  • Year and Date: 2010-12-16
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける弱い相互作用の効果 (2010)
  • Author(s): 高妻孝光
  • Organizer: 第21回日本化学会関東支部茨城地区研究交流会
  • Place of Presentation: 多賀市民会館(日立)
  • Year and Date: 2010-11-05
• [Presentation] 精密構造解析から見えるブルー銅タンパク質における弱い相互作用 (2010)
  • Author(s): 高妻孝光
  • Organizer: 第21回日本化学会関東支部茨城地区研究交流会
  • Place of Presentation: 多賀市民会館(日立)
  • Year and Date: 2010-11-05
• [Presentation] 超高分解能X線結晶構造解析によるヘムタンパク質シトクロームcの特異的構造変化 (2010)
  • Author(s): 高妻孝光
  • Organizer: 第21回日本化学会関東支部茨城地区研究交流会
  • Place of Presentation: 多賀市民会館(日立)
  • Year and Date: 2010-11-05
• [Presentation] Achromobacter cycloclastes由来亜酸化窒素還元酵素の電子移動反応 (2010)
  • Author(s): 高妻孝光
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-22
• [Presentation] Effect of Weak Interaction on the Electronic Structure and Electrochemical Properties of Pseudoazurin Met16His/His6Val double mutant (2010)
  • Author(s): 高妻孝光
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-21
• [Presentation] ブルー銅タンパク質シュウドアズリンの酸性条件下における構造転移 (2010)
  • Author(s): 高妻孝光
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-21
• [Presentation] X-ray crystal structure analysis of a blue copper protein, pseudoazurinat 1.35 A resoution (2010)
  • Author(s): 高妻孝光
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-21
• [Presentation] Cu K-edge X-ray Absorption Spectroscopic Studies of Blue Copper Protein, Plastocyanin from a Fern Plant, Dryopteris crasssrhizoma (2010)
  • Author(s): 高妻孝光
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-20
• [Presentation] Atomic Resolution X-ray crystallographic Analyses and Spectroscopic Studies of Cytochrome d from Alcaligenes xylosoxidans (2010)
  • Author(s): 高妻孝光
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-20
• [Presentation] Two step alkaline transition of stellacyanin from fthus vernicifera (2010)
  • Author(s): 高妻孝光
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-20
• [Presentation] Regulation of the Structure and Protein Stability through the Weak Interaction in the 2nd Coordination Sphere of a Blue Copper Protein, Pseudoazurin (2010)
  • Author(s): 高妻孝光
  • Organizer: Xl_th International Symposium of Bioinorganic Chemistry
  • Place of Presentation: Hotel Verde Montana Wellness & Spa(クドバ、ポーランド)
  • Year and Date: 2010-09-08
• [Presentation] 弱い化学的相互作用によるタンパク質の電子状態制御 (2010)
  • Author(s): 高妻孝光
  • Organizer: 第4回日本化学会関東支部大会
  • Place of Presentation: 筑波大学(つくば)
  • Year and Date: 2010-08-30
• [Presentation] Perturbation on the Active Site of a Blue Copper Protein, Pseudoazurin through Non-covalent, Weak Interaction (2010)
  • Author(s): 高妻孝光
  • Organizer: 10th European Conference of Biological Inorganic Chemistry
  • Place of Presentation: Aristotle University(テサロニケ、ギリシャ)
  • Year and Date: 2010-06-24
• [Presentation] ブルー銅タンパク質シュウドアズリンThr36Lys変異体の構造と性質 (2010)
  • Author(s): 高妻孝光
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 神奈川大学(横浜)
  • Year and Date: 2010-03-26
• [Presentation] Atomic resolution X-ray crystallographic analyses, spectroscopic and kinetic studies of a cytochrome c' from Alcaligenes xylosoxidans (2010)
  • Author(s): Akiko Takashina, Masaki Unnno, Takamitsu Kohzuma
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii Convetion Center(アメリカ)
• [Presentation] Roman Czernuszewicz Structure and Functions of Non-Covalent Weak Interaction Probed with a Blue Copper Protein (2010)
  • Author(s): Takamitsu Kohzuma, Yuji Obara, Yuko Nihei, Koyu Fujita, Doreen E. Brown, David M. Dooley
  • Organizer: Met16X Pseudoazurin Variants, 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii Convetion Center(アメリカ)
• [Presentation] Effect of Weak Interaction on the Electronic Structure and Electrochemical Properties of Pseudoazurin Met16X mutants (2010)
  • Author(s): Yuji Obara, Marzena B. Fitzpatrick, Roman S. Czernuszewicz, Takamitsu Kohzuma
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii Convetion Center(アメリカ)
• [Presentation] 超高分解能X線結晶構造解析によるヘムタンパク質シトクロームc'の特異的構造変化 (2010)
  • Author(s): 高階明子, 海野昌喜, 高妻孝光
  • Organizer: 第21回日本化学会関東支部茨城地区研究交流会
  • Place of Presentation: 東海
• [Presentation] Regulation of the Structure and Protein Stability through the Weak Interaction in the 2nd Coordination Sphere of a Blue Copper Protein (2010)
  • Author(s): T. Kohzuma, Y. Nihei, S. Asamura, Y. Obara, and M. Unno
  • Organizer: Pseudoazurin, XI International Symposium
  • Place of Presentation: Kudowa(ポーランド)
• [Presentation] Regulation of the structure and protein stability through the weak interaction in the 2nd coordination sphere of metalloproteins (2010)
  • Author(s): T. Kohzuma
  • Organizer: 10th European Biological Inorganic Chemistry Conference
  • Place of Presentation: Thessalonike(ギリシャ)
• [Presentation] Atomic Resolution X-ray crystallographic Analyses and Spectroscopic Studies of a Cytochrome c' from Alcaligenes xylosoxidans (2010)
  • Author(s): 高階明子, 海野昌喜, 高妻孝光
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学
• [Presentation] Effect of Weak Interaction on the Electronic Structure and Electrochemical Properties of Pseudoazurin Met16His/His6Val double mutant (2010)
  • Author(s): 小原裕二、仁平裕子、大上利恵、高妻孝光
  • Organizer: 第48回生物物理学会年会
  • Place of Presentation: 東北大学
• [Presentation] 弱い化学的相互作用によるタンパク質の電子状態制御 (2010)
  • Author(s): 高妻孝光、仁平裕子、浅村紗矢香、小原裕二、海野昌喜
  • Organizer: 日本化学会関東支部
  • Place of Presentation: 筑波大学
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける弱い相互作用の効果 (2010)
  • Author(s): 高妻孝光、仁平裕子、浅村紗矢香、小原裕二、海野昌喜
  • Organizer: 第37回生体分子科学討論会
  • Place of Presentation: 山口大学
• [Book] 元素111の新知識 第2版増補版 (2013)
  • Author(s): 桜井 弘、荒野 泰、上山憲一、小谷 明、高妻孝光、佐治英郎、鈴木晋一郎、寺嶋孝仁、中山祐正、根矢三郎、羽場宏光、廣田 俊、藤井敏司、吉村哲彦 著
  • Total Pages: 463
  • Publisher: 講談社ブルーバックス