A novel biosensing mechanism using sensor kinase activity

• Project/Area Number: 22550149
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Nagoya University
• Principal Investigator: NAKAJIMA Hiroshi 名古屋大学, 大学院・理学研究科, 教授 (00283151)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 生体機能材料 / シグナル変換 / バイオセンシング / 電子伝達タンパク質 / センサーキナーゼ / アズリン / チトクロムc

Research Abstract

The objective of this study is to produce a novel bio-sensing mechanism constituted by sensor-kinase and signal transduction system that we proposed in previous works. Sensor-kinase is a protein which senses an external stimulus such as a simple molecule or light to phosphorylate a particular protein and regulate its activity. In this study, we attempted to transform the output of sensor-kinase to an electronic signal. We have almost achieved the initial objective by using a transcriptional regulator in place of sensor-kinase as a sensory moiety.

Research Products

• [Journal Article] Thermodynamic effects of the alteration of the axial ligand on the unfolding of the thermostable cytochrome c (2013)
  • Author(s): Rabindra KB., Nakajima, H., Jitumani R., Watanabe, Y., Mazumdar, S.
  • Journal Title: Biochemistry Volume: 52 Issue: 8 Pages: 1373-1384
  • DOI: 10.1021/bi300982v
  • Peer Reviewed
• [Journal Article] Cytochrome c552 from Thermus Thermophilus Engineered for Facile Substitution of Prosthetic Group (2011)
  • Author(s): Sk. Md. Ibrahim, H. Nakajima, K. Ramanathan, N. Takatani, T. Ohta, Y. Naruta, Y. Watanabe
  • Journal Title: Biochemistry Volume: 50(45) Issue: 45 Pages: 9826-9835
  • DOI: 10.1021/bi201048e
  • Peer Reviewed
• [Journal Article] Protein Photoconductors and Photodiodes (2011)
  • Author(s): Y. Tokita, Y. Goto, S. Yamada, T. Luo, H. Nakajima, Y. Watanabe
  • Journal Title: Angew. Chem. Int. Ed. Volume: 50 Issue: 49 Pages: 11663-11666
  • DOI: 10.1002/anie.201103341
  • Peer Reviewed
• [Journal Article] The role of the GAF and central domains of the transcriptional activator VnfA in Azotobacter vinelandii (2011)
  • Author(s): K.Yoshimitsu, N.Takatani, Y.Miura, Y.Watanabe, H.Nakajima
  • Journal Title: FEBS Journal Volume: 278 Issue: 18 Pages: 3287-3297
  • DOI: 10.1111/j.1742-4658.2011.08245.x
  • Peer Reviewed
• [Journal Article] Molecular Design of Heme Proteins for Future Application (2011)
  • Author(s): H. Nakajima, O. Shoji, Y. Watanabe
  • Journal Title: Catalysis Surveys from Asia Volume: 15 Issue: 2 Pages: 134-143
  • DOI: 10.1007/s10563-011-9117-9
  • Peer Reviewed
• [Journal Article] Rational engineering of Thermus thermophilus cytochrome c_<552> to a ther mally tolerant artificial peroxidase (2010)
  • Author(s): H.Nakajima, et al.
  • Journal Title: Dalton Trans. Volume: 39 Pages: 3105-3114
  • Peer Reviewed
• [Journal Article] Molecular Design of Heme Proteins for Future Application
  • Author(s): H.Nakajima, et al.
  • Journal Title: Catalysis Surveys from Asia Volume: 印刷中(未定)
  • Peer Reviewed
• [Presentation] Dynamic Control of Inter-proteins Interaction for Protein Based Signal Transduction System (2013)
  • Author(s): H. Nakajima
  • Organizer: Collaborative Conference on Materials Research 2013
  • Place of Presentation: Jeju, Korea
• [Presentation] An artificial Signal Transduction System Consisting of Electron Transfer Proteins (2013)
  • Author(s): H. Nakajima
  • Organizer: Campus Asia 3rd Meeting
  • Place of Presentation: Nanjing, China
• [Presentation] 金属タンパク質のダイナミクスを利用する機能の創出 (2013)
  • Author(s): 中島 洋
  • Organizer: 分子研研究会「生体配位化学の最前線と展望」
  • Place of Presentation: 岡崎
• [Presentation] シグナルトランスデューサーの化学 -機能解明と応用- (2013)
  • Author(s): 中島 洋
  • Organizer: 分子研研究会「生物物質科学の展望」
  • Place of Presentation: 岡崎
• [Presentation] シグナルトランスデューサータンパク質の化学 －機能解明と応用－ (2013)
  • Author(s): 中島 洋
  • Organizer: 分子研研究会「生物物質科学の展望」
  • Place of Presentation: 分子科学研究所、愛知
  • Invited
• [Presentation] 金属タンパク質のダイナミクスを利用する機能の創出 (2013)
  • Author(s): 中島 洋
  • Organizer: 分子研研究会「生体配位化学の最前線と展望」
  • Place of Presentation: 分子科学研究所、愛知
  • Invited
• [Presentation] An artificial Signal Transduction System Consisting of Electron Transfer Proteins (2013)
  • Author(s): H. Nakajima
  • Organizer: Campus Asia
  • Place of Presentation: Nanjing Univ., China
  • Invited
• [Presentation] 電子伝達タンパク質によるシグナル変換機構 －転写制御因子への利用－ (2013)
  • Author(s): 中島 洋、宮崎総司、渡辺芳人
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学、滋賀
• [Presentation] センサータンパク質に学ぶシグナル変換の仕組みづくり (2012)
  • Author(s): 中島洋
  • Organizer: 日本化学会第92春季年会(2012)
  • Place of Presentation: 慶応義塾大学(横浜)
  • Year and Date: 2012-03-26
• [Presentation] A Signal Transduction Mechanism Inspired by Natural Sensor Protein (2012)
  • Author(s): H.Nakajima
  • Organizer: Japan-India Workshop on "Biomedical Research"
  • Place of Presentation: 早稲田大学先端生命医科学センター(東京)(招待講演)
  • Year and Date: 2012-02-28
• [Presentation] A Signal Transduction Mechanism Inspired by Natural Sensor Protein (2012)
  • Author(s): 中島 洋
  • Organizer: JST 日印バイオ医学研究 ワークショップ
  • Place of Presentation: 東京
• [Presentation] 電子伝達タンパク質を利用する遺伝子転写制御因子の出力変換 (2012)
  • Author(s): 中島 洋
  • Organizer: 新学術領域「配位プログラミング」全体会議
  • Place of Presentation: 東京大学、東京
• [Presentation] アゾベンゼン修飾アズリンの外部刺激応答による酸化還元電位制御 (2012)
  • Author(s): 中島 洋、宮崎総司、渡辺芳人
  • Organizer: 第３９回生体分子科学討論会
  • Place of Presentation: 東北大学、宮城
• [Presentation] 置換活性なヘムを有するチトクロムcの調製と再構成 (2011)
  • Author(s): 中島洋
  • Organizer: 錯体化学会第61回討論会
  • Place of Presentation: 岡山理科大学(岡山)
  • Year and Date: 2011-09-17
• [Presentation] 部位特異的逐次修飾を利用した機能化アズリン修飾電極の調製 (2011)
  • Author(s): 中島洋
  • Organizer: 2011年電気化学秋季大会
  • Place of Presentation: 朱鷺メッセ・新潟コンベンションセンター(新潟)
  • Year and Date: 2011-09-09
• [Presentation] Signal Transduction System Consisting of Electron Transfer Proteins (2011)
  • Author(s): H.Nakajima
  • Organizer: ICBIC15
  • Place of Presentation: University of British Columbia (Vancouver, Canada)
  • Year and Date: 2011-08-08
• [Presentation] 置換活性なヘムを有するチトクロムc-生成、同定、再構成- (2011)
  • Author(s): 中島洋
  • Organizer: 第38回生体分子科学討論会
  • Place of Presentation: 筑波大学大学会館国際会議室(つくば)
  • Year and Date: 2011-06-23
• [Presentation] Engineering of Cytochrome c552 from a Thermophile (2011)
  • Author(s): H.Nakajima
  • Organizer: Nagoya University Global COE in Chemistry, 4th Annual Symposium
  • Place of Presentation: 名古屋大学(名古屋)
  • Year and Date: 2011-06-15
• [Presentation] 分離可能なヘムを有するチトクロムc-発現、精製、ヘムの分離- (2011)
  • Author(s): 中島洋
  • Organizer: 日本化学会第91春季年会(2011)
  • Place of Presentation: 日本化学会第91春季年会(2011)講演予稿集
  • Year and Date: 2011-03-11
• [Presentation] Engineering of Cytochrome c552 from a Thermophile (2011)
  • Author(s): H. Nakajima
  • Organizer: Nagoya University Global COE in Chemistry, 4th Annual Symposium
  • Place of Presentation: Nagoya
• [Presentation] Signal Transduction System Consisting of Electron Transfer Proteins (2011)
  • Author(s): H. Nakajima
  • Organizer: ICBIC15
  • Place of Presentation: Vancouver, Canada
• [Presentation] Functional analysis of VnfA, a nitrogenase transcriptional regulator from Azotobacter vinelandii (2010)
  • Author(s): H.Nakajima
  • Organizer: PACIFICHEM2010
  • Place of Presentation: Hawaii Convention Center, Honolulu, USA
  • Year and Date: 2010-12-16
• [Presentation] Protein Engineering of Cytochrome c_<552> from a thermophile (2010)
  • Author(s): H.Nakajima
  • Organizer: 10^<th> IRTG symposium
  • Place of Presentation: Munster Univ., Munster, Germany
  • Year and Date: 2010-11-30
• [Presentation] Artificial peroxidase founded on a thermally tolerant protein from Thermus thermophilus (2010)
  • Author(s): H.Nakajima
  • Organizer: 第60回記念錯体化学OSAKA国際会議
  • Place of Presentation: 大阪国際交流センター(大阪府)
  • Year and Date: 2010-09-28
• [Presentation] 電子伝達タンパク質-環境応答性分子複合体を用いた、シグナル変換の試み (2010)
  • Author(s): 中島洋
  • Organizer: 第20回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 徳島文理大学国際会議場(徳島県)
  • Year and Date: 2010-06-25
• [Presentation] Protein Engineering of Cytochrome c552 from a thermophile . A scaffold for artificial peroxidase (2010)
  • Author(s): H. Nakajima
  • Organizer: 10th IRTG joint symposium
  • Place of Presentation: Munster, Germany
• [Presentation] 電子伝達タンパク質-環境応答性分子複合体を用いた、シグナル変換の試み (2010)
  • Author(s): 中島 洋
  • Organizer: 第20回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 徳島
• [Remarks] ホームページ
  • URL: http://bioinorg.chem.nagoya-u.ac.jp/
• [Remarks] 生物無機化学研究室 - 名古屋大学
  • URL: http://bioinorg.chem.nagoya-u.ac.jp/
• [Remarks]
  • URL: http://www.chem.nagoya-u.ac.jp/005Overview/110/index.phtml.en