Project/Area Number |
22560290
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Power engineering/Power conversion/Electric machinery
|
Research Institution | Ariake National College of Technology |
Principal Investigator |
KONO Susumu 有明工業高等専門学校, 電気工学科, 准教授 (30270375)
|
Co-Investigator(Kenkyū-buntansha) |
TOMINAGA Nobuaki 有明工業高等専門学校, その他部局等, 教授 (30227631)
|
Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Akemi (90399262)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | パルスパワー / 物質導入 / 受精卵 / 極短パルス / メダカ / シクロヘキシミド / アクチノマイシンD / 成長遅れ / アクチノマイシン |
Research Abstract |
To establish a simple and efficient method to introduce materials into living organisms, pulse system was designed and tested. This system consists of a pulsed power generator to generate a high intensity voltage with very short pulse duration, several RC discharge circuits to generate low voltage pulses with long pulse duration, a trigger signal generator, and reactor. By using this system, chemical substances, such as cycloheximide and actinomycin D, or a fluorescent dye had been incorporated into fertilized Medaka eggs without damage inside them.
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