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New practical technology using pulsed power - material incorporation for fertilized eggs with a high intensity voltage with very short pulse duration

Research Project

Project/Area Number 22560290
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Power engineering/Power conversion/Electric machinery
Research InstitutionAriake National College of Technology

Principal Investigator

KONO Susumu  有明工業高等専門学校, 電気工学科, 准教授 (30270375)

Co-Investigator(Kenkyū-buntansha) TOMINAGA Nobuaki  有明工業高等専門学校, その他部局等, 教授 (30227631)
Co-Investigator(Renkei-kenkyūsha) YAMAGUCHI Akemi   (90399262)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsパルスパワー / 物質導入 / 受精卵 / 極短パルス / メダカ / シクロヘキシミド / アクチノマイシンD / 成長遅れ / アクチノマイシン
Research Abstract

To establish a simple and efficient method to introduce materials into living organisms, pulse system was designed and tested. This system consists of a pulsed power generator to generate a high intensity voltage with very short pulse duration, several RC discharge circuits to generate low voltage pulses with long pulse duration, a trigger signal generator, and reactor. By using this system, chemical substances, such as cycloheximide and actinomycin D, or a fluorescent dye had been incorporated into fertilized Medaka eggs without damage inside them.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (24 results)

All 2013 2012 2011 2010

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (17 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Development of Pulsed Power Supply for Material Incorporation into Medaka (Oryzias latipes) Eggs2012

    • Author(s)
      Takahiro Kubo, Akemi Yamaguchi, Nobuaki Tominaga, Susumu Kono
    • Journal Title

      Proceedings of 2^(nd) International Symposium on Technology for Sustainability (ISTS2012)

      Pages: 297-300

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of Pulsed Power Supply for Material Incorporation into Medaka (Oryzias latipes) Eggs2012

    • Author(s)
      Takahiro Kubo et al.
    • Journal Title

      2012 International Symposium on Technology for Sustainability (ISTS2012)

      Volume: - Pages: 297-300

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A study of material incorporation for Medaka (Oryzias latipes) eggs by various voltage pulses2011

    • Author(s)
      Susumu Kono, Akemi Yamaguchi, Takashi Tanabe, Nobuaki Tominaga, Hidenori Akiyama
    • Journal Title

      Proceedings of the 18th IEEE International Pulsed Power Conference

      Pages: 1165-1170

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Study of Material Incorporation for Medaka (Oryzias latipes) Eggs by Various Voltage Pulses2011

    • Author(s)
      Susumu Kono, et al
    • Journal Title

      Proc.of the 18th IEEE Int.Pulsed Power Conf.

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel material incorporation technique for Medaka (Oryzias latipes) eggs using nanosecond pulsed electric fields2010

    • Author(s)
      Nobuaki Tominaga, Susumu Kono, Akemi Yamaguchi, Hidenori Akiyama, Koji Arizono
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74(6) Pages: 1279-1282

    • NAID

      10027556606

    • URL

      http://dx.doi.org/10.1271/bbb.90958

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Material Incorporation Technique for Medaka (Oryzias latipes) Eggs Using Nanosecond Pulsed Electric Fields2010

    • Author(s)
      Nobuaki TOMINAGA, et al.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74(6) Pages: 1279-1282

    • NAID

      10027556606

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Improvement of Pulsed Power System for Incorporating Materials into Medaka (Oryzias latipes) Eggs2012

    • Author(s)
      Susumu Kono, Akemi Yamaguchi, Takahiro Kubo, Yasutaka Imamura, Nobuaki Tominaga
    • Organizer
      9th International Bioelectrics Symposium (BIOELECTRICS 2012)
    • Place of Presentation
      KKR Hotel Kumamoto (Kumamoto, Japan)
    • Year and Date
      2012-09-06
    • Related Report
      2012 Final Research Report
  • [Presentation] パルスパワーを用いたメダカ受精卵への物質導入実験におけるパラメータの検討2012

    • Author(s)
      久保貴博,山口明美,今村泰隆,冨永伸明,河野晋
    • Organizer
      平成23年度(第2回)高専卒業研究発表会
    • Place of Presentation
      熊本大学(熊本)
    • Year and Date
      2012-03-08
    • Related Report
      2012 Final Research Report
  • [Presentation] パルスパワーを用いたメダカ受精卵への物質導入実験におけるパラメータの検討2012

    • Author(s)
      久保貴博, 他
    • Organizer
      電気学会パルスパワー研究会
    • Place of Presentation
      熊本大学(熊本県)
    • Year and Date
      2012-03-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] パルスパワーを用いたメダカ受精卵への物質導入システムの改善2012

    • Author(s)
      久保貴博,今村泰隆,山口明美,冨永伸明,河野晋
    • Organizer
      平成23年度(第2回)高専卒業研究発表会
    • Place of Presentation
      久留米高専(福岡)
    • Year and Date
      2012-03-03
    • Related Report
      2012 Final Research Report
  • [Presentation] パルスパワーを用いたメダカ受精卵への物質導入システムの改善2012

    • Author(s)
      久保貴博, 他
    • Organizer
      電気学会九州支部平成23年度(第2回)高専卒業研究発表会
    • Place of Presentation
      久留米高専(福岡県)
    • Year and Date
      2012-03-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Improvement of Pulsed Power System for Incorporating Materials into Medaka (Oryzias latipes) Eggs2012

    • Author(s)
      Susumu Kono et al.
    • Organizer
      9th International Bioelectrics Symposium  (BIOELECTRICS 2012)
    • Place of Presentation
      Kumamoto, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高電界パルスによるメダカ卵への物質導入;分子量と導入効率についての検討2011

    • Author(s)
      山口明美,今村泰隆,河野晋,冨永伸明
    • Organizer
      第64回電気関係学会九州支部連合大会
    • Place of Presentation
      佐賀大学(佐賀)
    • Year and Date
      2011-09-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 極性の異なる2連続パルスによるメダカ卵への影響2011

    • Author(s)
      今村泰隆,山口明美,河野晋,冨永伸明
    • Organizer
      第64回電気関係学会九州支部連合大会
    • Place of Presentation
      佐賀大学(佐賀)
    • Year and Date
      2011-09-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 高電界パルスによるメダカ卵への物質導入;分子量と導入効率についての検討2011

    • Author(s)
      山口明美, 他
    • Organizer
      第64回電気関係学会九州支部連合大会
    • Place of Presentation
      佐賀大学(佐賀県)
    • Year and Date
      2011-09-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 極性の異なる2連続パルスによるメダカ卵への影響2011

    • Author(s)
      今村泰隆, 他
    • Organizer
      第64回電気関係学会九州支部連合大会
    • Place of Presentation
      佐賀大学(佐賀県)
    • Year and Date
      2011-09-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] メダカ卵内への化学物質導入量の増加を目的とした2種類の電圧パルスの連続印加実験2011

    • Author(s)
      河野晋,山口明美,冨永伸明
    • Organizer
      平成23年電気学会基礎・材料・共通部門大会
    • Place of Presentation
      東京工業大学(東京都)
    • Year and Date
      2011-09-21
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 異なる2種類のパルス電圧の連続印可によるメダカ受精卵への物質導入2011

    • Author(s)
      河野晋,山口明美,田邉貴志,冨永伸明
    • Organizer
      平成23年電気学会全国大会
    • Place of Presentation
      大阪大学(大阪)
    • Year and Date
      2011-03-18
    • Related Report
      2012 Final Research Report
  • [Presentation] 異なる高電界矩形波パルスによるメダカ受精卵への物質導入について2011

    • Author(s)
      山口明美,河野晋,田邉貴志,冨永伸明
    • Organizer
      平成23年電気学会全国大会
    • Place of Presentation
      大阪大学(大阪)
    • Year and Date
      2011-03-18
    • Related Report
      2012 Final Research Report
  • [Presentation] 異なる2種類のパルス電圧の連続印可によるメダカ受精卵への物質導入2011

    • Author(s)
      河野晋, 他
    • Organizer
      平成23年電気学会全国大会
    • Place of Presentation
      大阪大学(大阪)(※中止。発表は成立。)
    • Year and Date
      2011-03-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 異なる高電界矩形波パルスによるメダカ受精卵への物質導入について2011

    • Author(s)
      山口明美, 他
    • Organizer
      平成23年電気学会全国大会
    • Place of Presentation
      大阪大学(大阪)(※中止。発表は成立。)
    • Year and Date
      2011-03-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 化学物質評価への応用を目指したパルスパワーによるメダカ受精卵への化学物質の新規導入法2011

    • Author(s)
      冨永伸明
    • Organizer
      バイオエレクトリクスシンポジウム2011
    • Place of Presentation
      熊本大学(熊本)
    • Year and Date
      2011-03-10
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] パルスパワーを用いたメダカ卵への物質導入に関する基礎研究2010

    • Author(s)
      田邉貴志,山口明美,冨永伸明,河野晋
    • Organizer
      平成22年度(第63回)電気関係学会九州支部連合大会
    • Place of Presentation
      九州産業大学(福岡)
    • Year and Date
      2010-09-25
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 物質導入方法およびその装置2013

    • Inventor(s)
      河野晋,冨永伸明,山口明美
    • Industrial Property Rights Holder
      独立行政法人国立高等専門学校機構
    • Filing Date
      2013-06-06
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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