Study of genes regulated by heterotrimeric G protein in rice

• Project/Area Number: 22570048
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Fukui Prefectural University
• Principal Investigator: IWASAKI Yukimoto 福井県立大学, 生物資源学部, 教授 (20193732)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: RNAi 法 / イネ / 細胞分裂 / 3量体Gタンパク質 / シグナル伝達 / ブラシノステロイド / 植物 / ３量体Ｇタンパク質 / RNAi 法 / d1 / 細胞数 / αサブユニット / βサブユニット / RNAi法

Research Abstract

The rice mutant for heterotrimeric G protein α subunit gene d1 showed dwarf. The cause of dwarf in d1 was due to the reduction of cell number. By microarray analysis, transcripts for eleven genes, which may be concerned with cell division, were suppressed in d1. In reduction of cell number in d1, two hypotheses are considered, the former is reduction of cell division frequency and the latter is the impaired vertical development due to abnormal orientation of cell division.

Research Products

• [Journal Article] Rice transgenic plants with suppressed expression of the β subunit of the heterotrimeric G protein (2012)
  • Author(s): Utsunomiya, Y., Samejima, C., Fujisawa, Y., Kato, H. and Iwasaki, Y
  • Journal Title: Plant Signaling Behavior Volume: 7 Pages: 443-446
  • Peer Reviewed
• [Journal Article] Identification of heterotrimeric G protein α and βsubunits in rice (2012)
  • Author(s): Abe, Y., Matsusita, K., Komatu, S. and Iwasaki. Y
  • Journal Title: Protein Peptide Letters Volume: 19 Pages: 277-281
  • Peer Reviewed
• [Journal Article] Rice transgenic plants with suppressed expression of the β subunit of the heterotrimeric G protein. (2012)
  • Author(s): Utsunomiya, Y., Samejima, C., Fujisawa, Y., Kato, H. and Iwasaki, Y.(Iwasaki)
  • Journal Title: Plant Signaling Behavior Volume: 7 Pages: 443-446
  • Peer Reviewed
• [Journal Article] Identification of heterotrimeric G protein α and β subunits in rice (2012)
  • Author(s): Abe, Y., Matsusita, K., Komatu, S. and Iwasaki. Y. (Iwasaki)
  • Journal Title: Protein Peptide Letters Volume: 19 Pages: 277-281
  • Peer Reviewed
• [Journal Article] Suppression of the rice heterotrimeric G protein β-subunit gene, RGB1, causes dwarfism and browning of internodes and lamina joint regions (2011)
  • Author(s): Utsunomiya, Y., Samejima, C., Takayanagi, Y., Izawa, Y., Yoshida, T., Sawada, Y., Fujisawa, Y., Kato, H. and Iwasaki, Y
  • Journal Title: Plant J Volume: 67 Pages: 907-916
  • Peer Reviewed
• [Journal Article] Expression profile of the α subunit of the heterotrimeric G protein in rice (2010)
  • Author(s): Izawa, Y., Minami, M., Ohki, S.and Iwasaki Y
  • Journal Title: Plant Signaling Behavior Volume: 5 Pages: 1-3
  • Peer Reviewed
• [Journal Article] Function and expression pattern of the α subunit of the heterotrimeric G protein in rice (2010)
  • Author(s): Izawa, Y., Takayanagi, Y., Inaba, N., Abe, Y., Minami, M., Fujisawa, Y., Kato, H., Ohki, S., Kitano, H., and Iwasaki Y
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 271-281
  • NAID: 10027346850
  • Peer Reviewed
• [Presentation] Function of rice heterotrimeric G protein (2013)
  • Author(s): Iwasaki, Y
  • Organizer: Japan-China Joint Symposium on Rice Developmental Biology ーFrom Morphogenesis to Yield.
  • Place of Presentation: Beppu, Oita, Japan
• [Presentation] 短粒で矮性を示すイネ3 量体G タンパク質aサブユニット欠損変異体(d1) とブラシノステロイド関連変異体の組織学的な形態の比較 (2013)
  • Author(s): 瀬上修平、井沢 有希、香野みずき、北野英己、三浦孝太郎、岩崎行玄
  • Organizer: 日本育種学会 第123回講演会
  • Place of Presentation: 東京農業大学
• [Presentation] Function of rice heterotrimeric G protein (2013)
  • Author(s): Iwasaki, Y.
  • Organizer: Japan-China Joint Symposium on Rice Developmental Biology
  • Place of Presentation: 別府
  • Invited
• [Presentation] 短粒で矮性を示すイネ3 量体G タンパク質αサブユニット欠損変異体(d1) とブラシノステロイド関連変異体の組織学的な形態の比較 (2013)
  • Author(s): 瀬上修平、井沢 有希、香野みずき、北野英己、三浦孝太郎、岩崎行玄
  • Organizer: 日本育種学会 第123回講演会
  • Place of Presentation: 東京
• [Presentation] イネの種子形を制御する遺伝子 (2012)
  • Author(s): 岩崎行玄、三浦孝太郎、瀬上修平
  • Organizer: 日本育種学会122回講演会 ワークショップ
  • Place of Presentation: 京都
• [Presentation] Study of heterotrimeric G protein complex in rice (2012)
  • Author(s): Abe, Y., Izawa, Y., Utsunomiya, U., Kato, H. and Iwasaki, Y.
  • Organizer: Gordon Research Conferences, Plant Molecular Biology.
  • Place of Presentation: NH, USA
• [Presentation] イネの種子形を制御する遺伝子 (2012)
  • Author(s): 岩崎行玄、三浦孝太郎、瀬上修平
  • Organizer: 日本育種学会１２２回講演会 ワークショップ
  • Place of Presentation: 京都
• [Presentation] Study of heterotrimeric G protein complex in rice. Gordon Research Conferences, Plant Molecular Biology (2012)
  • Author(s): Abe, Y., Izawa, Y., Utsunomiya, U., Kato, H. and Iwasaki, Y.
  • Organizer: Gordon Research Conferences
  • Place of Presentation: NH, USA
• [Presentation] イネ3量体Gタンパク質αサブユニットは、葉鞘の細胞数の制御において、ブラシノステロイドシグナリングの下流で機能する (2011)
  • Author(s): 井沢有希,香野みずき,北野英己,三浦孝太郎,岩崎行玄
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] イネ3量体Gタンパク質βサブユニットの機能解析 (2011)
  • Author(s): 宇都宮有瑞子,鮫島千裕,高柳欣幸,井沢有希,吉田貴寿,藤澤由紀子,加藤久晴,岩崎行玄
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] 縦方向の伸長を制御するイネ遺伝子群の解析 (2010)
  • Author(s): 香野みずき、井沢有希、北野英己、三浦孝太郎、岩崎行玄
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 秋田
• [Presentation] Analysis of β subunit of Rice Heterotrimeric G Protein (2010)
  • Author(s): Utsunomiya, U., Samejima, C., Fujisawa, Y., Kato, H. and Iwasaki, Y
  • Organizer: XVII Congress of the Federation of European Societies of Plant Biology (FESPB)
  • Place of Presentation: Valencia, Spain
• [Presentation] Function and expression of the α subunit of rice heterotrimeric G protein (2010)
  • Author(s): Izawa, Y., Yoshiyuki Takayanagi, Y., Inaba, N., Abe, Y., Minami, M., Fujisawa, Y., Kato, H., Ohki, S., Kitano, H. Iwasaki, Y
  • Organizer: XVII Congress of the Federation of European Societies of Plant Biology (FESPB)
  • Place of Presentation: Valencia, Spain