| Project/Area Number |
22570051
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
MASA H Sato 京都府立大学, 大学院・生命環境科学研究科, 准教授 (20283575)
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| Co-Investigator(Renkei-kenkyūsha) |
FUKAO Youichiro 奈良先端科学技術大学院大学, バイオサイエンス研究科, 特任助教 (80432590)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 植物分子機能 / 先端成長 / SNARE / 細胞の極性成長 / 根毛 / SYP123 / 細胞骨格 / メンブレントラフィック / 細胞内小胞輸送 / 細胞極性確立 / シロイヌナズナ / 根毛細胞 / 極性輸送 |
|---|
| Research Abstract |
In this study, we investigated the function and subcellular localization of SH3Ps using T-DNA insertion or RNAi knockdown mutants and transgenic plants expressing GFP-fusion proteins. We demonstrated that SH3P1-GFP was localized to various organelles including the plasma membrane, transport vesicles as well as the cell plate of dividing cells. In particular, SH3P1-GFP showed polar localization similar to the localization of PIN2 in epidermal cells, and an actin polymerization inhibitor, latrunculinB, a PI3P kinase inhibitor, wortmannin affected the polarized localization SH3P1. This result indicates that the polar localization of SH3P1-GFP depended on actin cytoskeleton and the endocytotic pathway.
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