The structure-function relationships of rice chitin elicitor receptors in plant innate immunity
Project/Area Number |
22570052
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Meiji University |
Principal Investigator |
KAKU HANAE 明治大学, 農学部, 教授 (70409499)
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Co-Investigator(Renkei-kenkyūsha) |
SHIBUYA Naoto 明治大学, 農学部, 教授 (70350270)
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Project Period (FY) |
2010-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | イネ / キチン受容体 / MAMP / キチンオリゴ糖 / CEBiP / OsCERK1 / 防御応答 / 過剰発現 / 植物免疫 / MAMPs |
Research Abstract |
Plants have the ability to recognize microbe/pathogen-associated molecular patterns (MAMPs/ PAMPs) and initiate various defense responses. Chitin, a major component of fungal cell wall, is a representative fungal MAMP and triggers defense signaling in a wide range of plant species. We identified two types of LysM-receptors, CEBiP and OsCERK1, which play important roles for chitin oligosaccharide-induced defense signaling in rice. In this study, we found that the heteromeric receptor complex of CEBiP and OsCERK1 is essential to activate its downstream signaling in rice and GPI anchored protein, CEBiP, is a major protein for ligand perception. We also found that one of the LysM domains in the extracellular region and the isoleucine residue in position 122 are the critical site/position for chitin binding in CEBiP. We also indicated that the sandwich typed dimerization of CEBiP is required for chitin mediated signal transduction in rice.
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Report
(5 results)
Research Products
(123 results)
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[Journal Article] Chitin-induced activation of immune signaling by the rice receptor CEBiP relies on a unique sandwich-type dimerization2014
Author(s)
M. Hayafunea, R. Berisiob, R. Marchettic, A. Silipoc, M. Kayamaa, Y. Desakia, S. Arimaa, F. Squegliab, A. Ruggierob, K. Tokuyasud, A. Molinaroc, H. Kaku, N. Shibuya
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 111
Related Report
Peer Reviewed
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[Journal Article] Chitin-induced activation of immune signaling by the rice receptor CEBiP relies on a unique sandwich-type dimerization2014
Author(s)
M. Hayafunea, R. Berisiob, R. Marchettic, A. Silipoc, M. Kayamaa, Y. Desakia, S. Arimaa, F. Squegliab, A. Ruggierob, K. Tokuyasud, A. Molinaroc, H. Kaku, N. Shibuya
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 111
Related Report
Peer Reviewed
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[Journal Article]2011
Author(s)
T. Nakagawa, H. Kaku, Y. Shimoda, A. Sugiyama, M. Shimamura, K. Takanashi, K. Yazaki, T. Aoki, N. Shibuya, H. Kouchi
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Journal Title
Plant J.
Volume: 65
Pages: 169-180
Related Report
Peer Reviewed
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[Journal Article] Two LysM receptor molecules, CEBiP and OsCERK1, cooperatively regulate chitin elicitor signaling in rice2010
Author(s)
T. Simizu, T. Nakano, D. Takamizawa,Y. Desaki, N. Ishii-Minami, Y. Nishizawa, E. Minami, K. Okada, H. Yamane, H. Kaku, N. Shibuya
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Journal Title
Plant J.
Volume: 64
Pages: 204-214
Related Report
Peer Reviewed
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[Presentation] イネキチン受容体CEBiPのリガンド認識機構2014
Author(s)
早船真広、出﨑能丈、加山実祐、谷本匠、R. Berisio, R. Marchetti, A. Silipo, F. Squeglia, A. Ruggiero, 徳安健, Antonio Molinaro, 渋谷直人、賀来華江
Organizer
第55回日本植物生理学会年会
Place of Presentation
富山大学
Related Report
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[Presentation] Ligand recognition, autophosphorylation and signaling by plant chitin receptors2013
Author(s)
N. Shibuya, H. Kaku, T. Shinya, K. Kito, N. Motoyama, M. Hayafune, R. Berisio, A. Silipo, A. Molinaro, K. Yamaguchi, T. Kawasaki
Organizer
KEYSTONE SYMPOSIA Plant immunity : Pathways and Translation
Related Report
Invited
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[Presentation] イネキチン受容体キナーゼOsCERK1の過剰発現は植物免疫応答を強化する2013
Author(s)
中道研地,出崎能丈,大友一平,中谷容子,成澤知子,香西雄介,青柳伸代,西澤洋子,南栄一,賀来華江,澁谷直人
Organizer
第54回日本植物生理学会年会
Related Report
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[Presentation] Ligand recognition, autophosphorylation and signaling by plant chitin receptors2013
Author(s)
Naoto Shibuya, Hanae Kaku, Tomonori Shinya, Keiji Kito, Noriko Motoyama, Masahiro Hayafune, Rita Berisio, Alba Silipo, Antonio Molinaro, Koji Yamaguchi, Tsutomu Kawasaki.
Organizer
KEYSTONE SYMPOSIA Plant immunity: Pathways and Translation
Place of Presentation
ビッグスカイリゾート・米国
Related Report
Invited
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[Presentation] Overexpression of rice chitin elicitor receptor kinase OsCERK1 improved immune responses in rice2013
Author(s)
Yoshitake Desaki, Kenji Nakamichi, Ippei Otomo, Hiroko Nakatani, Tomoko Narisawa, Yusuke Kouzai, Nobuyo Aoyagi, Yoko Nishizawa, Eiichi Minami, Hanae Kaku, Naoto Shibuya
Organizer
KEYSTONE SYMPOSIA Plant immunity: Pathways and Translation
Place of Presentation
ビッグスカイリゾート・米国
Related Report
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[Presentation] MAMPsシグナリングにおける受容体様細胞質キナーゼAtRLCK1の機能解析2013
Author(s)
新屋友規, 山口公志, 出﨑能丈, 成澤知子, 前田佳菜子, 小林佳弘, 鈴木丸陽, 谷本匠, 竹田潤, 船間亮汰, 山田健太, 鳴坂真理, 鳴坂義弘, 賀来華江, 川﨑努, 渋谷直人
Organizer
平成25年度 植物病理学会 関西部会
Place of Presentation
岡山大学
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[Presentation] Chitin Receptors in Plant Immunity2012
Author(s)
Naoto Shibuya, (Hanae Kaku), Tomonori Shinya, Takeo Shimizu*, Tomomi Nakagawa, Noriko Motoyama
Organizer
XVth International Congress on Molecular Plant-Microbe Interactions
Place of Presentation
Kyoto, Japan
Related Report
Invited
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[Presentation] MAMP-mediated priming and synergy : Closely related positive crosstalk of signaling cascade for effective mobilization of defense responses2011
Author(s)
Yoshitake Desaki, Ippei Otomo, Daijiro Kobayashi, Yusuke Jikumaru, Yuji Kamiya, Balakrishnan Venkatesh, Shinji Tsuyumu, Hiroshi Takatsuji, Hanae Kaku, Naoto Shibuya
Organizer
MCGS Network Symposium
Place of Presentation
University of Munster,ドイツ
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