Visualization of peroxisomes and microtubules by GFP marker in a green alga Klebsormidium

• Project/Area Number: 22570060
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: The University of Tokyo
• Principal Investigator: HASHIMOTO Haruki 東京大学, 大学院・総合文化研究科, 准教授 (90134410)
• Co-Investigator(Kenkyū-buntansha): FUJIWARA Makoto 上智大学, 理工学部, 准教授 (90332345)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: クレブソルミディウム / 形質転換 / GFP / ペルオキシソーム / 微小管 / マイクロボディ / パーティクルボンバードメント / 細胞周期 / 緑藻 / パーティクボンバードメント / 電気穿孔法

Research Abstract

Exogenous expression of GFP marker genes by particle bombardment methods were employed in order to visualize peroxisomes and microtubules in a green alga Klebsormidium flaccidum. Two GFP-fluoresced cells were observed under the following settings: the Closterium CAB1 or CMV 35S promoters, 0.4 μm particles, 650 psi pressure, and a 12 cm distance for the gold particle bombardment.

Research Products

• [Journal Article] Role of galactolipid biosynthesis in coordinated development of photosynthetic complexes and thylakoid membranes during chloroplast biogenesis in Arabidopsis. (2013)
  • Author(s): Kobayashi, K., Narise, T., Sonoike, K., Hashimoto, H. 他
  • Journal Title: The Plant Journal Volume: 73 Pages: 250-261
  • Peer Reviewed
• [Journal Article] Characterization of a heavy-ion induced white flower mutant of allotetraploid Nicotiana tabacum. (2013)
  • Author(s): Kazama Y, Fujiwara MT, Takehisa H, Ohbu S., Saito H, Ichida H, Hayashi Y, Abe T.他
  • Journal Title: Plant Cell Reports Volume: 32(1) Issue: 1 Pages: 11-19
  • DOI: 10.1007/s00299-012-1336-7
  • Peer Reviewed
• [Journal Article] Role of galactolipid biosynthesis in coordinated development of photosynthetic complexes and thylakoid (2013)
  • Author(s): (1) Kobayashi, K. et al.
  • Journal Title: The Plant Journal Volume: 73 Pages: 250-261
  • Peer Reviewed
• [Journal Article] SlWUS1; A novel X-linked gene having no homologous Y-linked copy in Silene latifolia (2012)
  • Author(s): Kazama, Y., Nishihara, K., Fujiwara, M.T. Abe, T., and Kawano, S
  • Journal Title: G3 Volume: 2 Issue: 10 Pages: 1269-1278
  • DOI: 10.1534/g3.112.003749
  • Peer Reviewed
• [Journal Article] Visualization of plastid movement in the pollen tube of Arabidopsis thaliana. (2012)
  • Author(s): Fujiwara, M.T., Yoshioka, Y., Hirano, T., Kazama. Y., Abe, T., Hayashi, K., Itoh, R.D.
  • Journal Title: Plant Signaling Behavior Volume: vol. 7 (1) Issue: 1 Pages: 34-37
  • DOI: 10.4161/psb.7.1.18484
  • Peer Reviewed
• [Journal Article] Visualization of plastid movement in the pollen tube of Arabidopsis thaliana. (2012)
  • Author(s): Fujiwara, M.T. et al.
  • Journal Title: Plant Sigmaling & Behavior Volume: 7 Pages: 34-37
  • Peer Reviewed
• [Journal Article] Visualization of plastid movement in the pollen tube of Arabidopsis thaliana (2012)
  • Author(s): Fujiwara, M.T., et al.
  • Journal Title: Plant Signaling & Behavior Volume: 7 Pages: 34-37
  • Peer Reviewed
• [Journal Article] Dynamic morphologies of pollen plastids visualized by vegetative-specific FtsZ1-GFP in Arabidopsis thaliana. (2010)
  • Author(s): Fujiwara, M.T., Hashimoto, H., Kazama, Y. 他.
  • Journal Title: Protoplasma Volume: 242 Issue: 1-4 Pages: 19-33
  • DOI: 10.1007/s00709-010-0119-7
  • Peer Reviewed
• [Journal Article] Regulation of leucoplast morphology in roots: interorganellar signaling from mitochondria? (2010)
  • Author(s): Itoh RD, Fujiwara MT .
  • Journal Title: Plant Signaling Behavior Volume: 5(7) Pages: 856-859
  • Peer Reviewed
• [Journal Article] Chemical induction of rapid and reversible plastid filamentation in Arabidopsis thaliana roots. (2010)
  • Author(s): Itoh RD, Yamasaki H, Septiana A, Yoshida S, Fujiwara MT.
  • Journal Title: Physiologia Plantarum Volume: 139(2) Pages: 144-158
  • Peer Reviewed
• [Presentation] 細胞内共生説に魅せられて (2012)
  • Author(s): 箸本春樹
  • Organizer: 日本植物形態学会第24回大会
  • Place of Presentation: 姫路
  • Year and Date: 2012-09-14
• [Presentation] Klebsormidium flaccidum の細胞周 期における微小管の動態 (2011)
  • Author(s): 山本弘貴, 箸本 春樹 緑藻
  • Organizer: 日本植物形態学会第23回大会
  • Year and Date: 2011-09-16
• [Presentation] 緑藻Klebsormidium flaccidumの細胞周期における微小管の動態 (2011)
  • Author(s): 山本弘貴, 箸本春樹
  • Organizer: 日本植物形態学会第23回大会
  • Place of Presentation: 東京・日本女子大学
  • Year and Date: 2011-09-16
• [Presentation] Electron microscopic study on division of secondary plastids surrounded by four membranes in chromophyte algae. (2011)
  • Author(s): Hashimoto, Haruki
  • Organizer: VI European Congress of Protistology
  • Place of Presentation: ベルリン
  • Year and Date: 2011-07-25
• [Presentation] Electron microscopic study on division of secondary plastids surrounded by four membranes in chromophyte algae (2011)
  • Author(s): Haruki Hashimoto
  • Organizer: VI European congress of protistology
  • Place of Presentation: ドイツ・ベルリン自由大学
  • Year and Date: 2011-07-25
• [Presentation] Plastid replication in leaf epidermisInsights from the atminE1 mutant of Arabidopsis (2011)
  • Author(s): Fujiwara MT, et al.'
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-22
• [Presentation] Plastid replication in leaf epidermis: Insights from the atminE1 mutant of Arabidopsis (2010)
  • Author(s): Fujiwara MT, et al.
  • Organizer: The 21st International Conference onArabidopsis Research (ICAR2010)
  • Place of Presentation: Yokohama
  • Year and Date: 2010-06-07
• [Presentation] 細胞内共生説に魅せられて
  • Author(s): 箸本春樹
  • Organizer: 日本植物形態学会第24回大会
  • Place of Presentation: 兵庫県立大学（兵庫県姫路市）
• [Remarks]
  • URL: http://librsh01.lib.sophia.ac.jp/Profiles/70/0006951/profile.html