Visualization of peroxisomes and microtubules by GFP marker in a green alga Klebsormidium
Project/Area Number |
22570060
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Morphology/Structure
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Research Institution | The University of Tokyo |
Principal Investigator |
HASHIMOTO Haruki 東京大学, 大学院・総合文化研究科, 准教授 (90134410)
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Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Makoto 上智大学, 理工学部, 准教授 (90332345)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | クレブソルミディウム / 形質転換 / GFP / ペルオキシソーム / 微小管 / マイクロボディ / パーティクルボンバードメント / 細胞周期 / 緑藻 / パーティクボンバードメント / 電気穿孔法 |
Research Abstract |
Exogenous expression of GFP marker genes by particle bombardment methods were employed in order to visualize peroxisomes and microtubules in a green alga Klebsormidium flaccidum. Two GFP-fluoresced cells were observed under the following settings: the Closterium CAB1 or CMV 35S promoters, 0.4 μm particles, 650 psi pressure, and a 12 cm distance for the gold particle bombardment.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Visualization of plastid movement in the pollen tube of Arabidopsis thaliana.2012
Author(s)
Fujiwara, M.T., Yoshioka, Y., Hirano, T., Kazama. Y., Abe, T., Hayashi, K., Itoh, R.D.
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Journal Title
Plant Signaling Behavior
Volume: vol. 7 (1)
Issue: 1
Pages: 34-37
DOI
Related Report
Peer Reviewed
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