Structural studies of a chromoprotein from Pleurotus Salmoneostramineus L.Vass

• Project/Area Number: 22570123
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: University of Hyogo
• Principal Investigator: SHIBATA Naoki 兵庫県立大学, 大学院・生命理学研究科, 准教授 (30295753)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 色素タンパク質 / 結晶構造解析 / 担子菌 / 酸素発生 / タンパク質 / 色素

Research Abstract

In this study cDNA coloning, purification, and X-ray structureanalysis of a chromoprotein from Pleurotus Salmoneostramineushave been performed. The X-ray structure revealed that the chromoprotein does not contain the reported pigment molecule but does a novel linear chromophore which might lei along the active site of it.

Research Products

• [Presentation] Primer design for cDNA synthesis based on the crystal structure、XXII Congress and General Assembly (2011)
  • Author(s): 柴田直樹・井上豪・樋口芳樹・甲斐泰
  • Organizer: International Union of Crystallography
  • Place of Presentation: マドリッド(スペイン)
• [Presentation] 結晶構造に基づくトキイロヒラタケ由来色素タンパク質の遺伝子クローニング (2011)
  • Author(s): 柴田直樹・井上豪・武隈秀子・武隈真一・吉田善一・樋口芳樹・甲斐泰
  • Organizer: 平成 23 年度日本結晶学会年会
  • Place of Presentation: 北海道大学(北海道)
• [Presentation] Primer design for cDNA synthesis based on the crystal structure (2011)
  • Author(s): 柴田直樹
  • Organizer: IUCr 2011
  • Place of Presentation: マドリッド(スペイン)
• [Presentation] 結晶構造に基づくトキイロヒラタケ由来色素タンパク質の遺伝子クローニング (2011)
  • Author(s): 柴田直樹
  • Organizer: 平成23年度日本結晶学会年会
  • Place of Presentation: 北海道大学(北海道)
• [Remarks]
  • URL: http://www.sci.u-hyogo.ac.jp/life/biophys1/index-j.html