| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
To better understand of mechanism of protein C-mannosylation, we tried to develop a simple approach to assess this modification of cell lines. We have used site-directed mutagenesis to alter the tryptophan, a predicted C-mannosylation site in TSR protein A, to alanine or phenylalanine. Both mutations resulted in decreased secretion of the protein, andthe mutants were retained in the endoplasmic reticulum. These results suggested the possibility that the secretion of the protein may representthe protein C-mannosylation in the cell lines.
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