Development of an artificial hyperthermophilic cellulase toward a hydrolysis of crystalline cellulose.
| Project/Area Number |
22570128
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
UEGAKI Koichi 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究グループ長 (00356544)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Tsutomu 産業技術総合研究所, 健康工学研究部門, 主任研究員 (10357668)
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| Co-Investigator(Renkei-kenkyūsha) |
IKEGAMI Takahisa 大阪大学, 生命機能研究科, 准教授 (20283939)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | タンパク質 / バイオマス / セルラーゼ / 人工酵素 / 高機能化 / セルロース / キチナーゼ / 融合酵素 / ドメイン / 耐熱性 / 糖分解酵素 / リンカー長 |
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| Research Abstract |
The artificial hyperthermophilic cellulase was created by adding a hyperthermophilic carbohydrate-binding module (CBM) of chitinase from P. furiosus to the C-terminal region of hyperthermophilic cellulase of P. horikoshii. In this project, the effect of l
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Report
(4 results)
Research Products
(76 results)
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[Journal Article] Expression, refolding, and purification of active diacetylchitobiose deacetylase from Pyrococcus horikoshii2012
Author(s)
Mine, S., Ikegami, T., Kawasaki, K., Nakamura, T., Uegaki, K
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Journal Title
Protein Expr Purif
Volume: Vol.84, No.2
Issue: 2
Pages: 265-269
DOI
Related Report
Peer Reviewed
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[Journal Article] Application of a water jet system to the pretreatment of cellulose2011
Author(s)
Watanabe, Y., Kitamura, S., Kawasaki, K., Kato, T., Uegaki, K., Ogura, K., Ishikawa,K
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Journal Title
Biopolymers
Volume: Vol.95, No.12
Issue: 12
Pages: 833-839
DOI
Related Report
Peer Reviewed
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[Journal Article] N-terminal phosphorylation of HP1{alpha} promotes its chromatin binding2011
Author(s)
Hiragami-Hamada, K., Shinmyozu, K., Hamada, D., Tatsu, Y., Uegaki, K., Fujiwar,a S., Nakayama, J.I
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Journal Title
Mol Cell Biol
Volume: Vol.31, No.6
Issue: 6
Pages: 1186-1200
DOI
Related Report
Peer Reviewed
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[Journal Article] Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus-the role of conserved residues in the active site2010
Author(s)
Tsuji, H.,, Nishimura 、 S., Inui1T,, Kado, Y., Ishikawa, K., Nakamuram T.,Uegaki, K
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Journal Title
FEBS J
Volume: Vol.277, No.12
Issue: 12
Pages: 2683-2695
DOI
Related Report
Peer Reviewed
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