Study on NADPH oxidase function in development

• Project/Area Number: 22570140
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: KAMEI Kaeko 京都工芸繊維大学, 工芸科学研究科, 教授 (00214544)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Masamitsu 京都工芸繊維大学, 工芸科学研究科, 教授 (00182460)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 昆虫 / ショウジョウバエ / NADPHオキシダーゼ / 活性酸素種 / 発生 / 翅形成 / Nox/Duox

Research Abstract

NADPH (nicotinamide adenine dinucleotide phosphate) oxidase is an enzyme　that produces reactive oxygen species. The function of NADPH oxidase in the　development, especially in the wing formation, was analyzed using Drosophila.　We reveled that NADPH oxidase is essential for normal wing development　through the melanin formation and cross-linking of tyrosine for wing　sclerotization and the suppression of apoptosis. By genetic screening, the　genetic region on 3 rd chromosome that could interact with dDuox (catalytic　domain of NADPH oxidase) was found.

Research Products

• [Journal Article] Essential role of Duox in stabilization of Drosophila wing (2011)
  • Author(s): Nguyen Thi Tu Anh, Maiko Nishitani, Shigeharu Harada, Masamitsu Yamaguchi, and Kaeko Kamei
  • Journal Title: The Journal of Biological Chemistry Volume: 286(38) Pages: 33244-33251
  • Peer Reviewed
• [Journal Article] A Drosophila model for the screening of bioavailable NADPH oxidase inhibitors and antioxidants (2011)
  • Author(s): Nguyen Thi Tu Anh, Maiko Nishitani, Shigeharu Harada, Masamitsu Yamaguchi, and Kaeko Kamei.
  • Journal Title: Molecular and Cellular Biochemistry Volume: 352(1-2) Pages: 91-98
  • Peer Reviewed
• [Journal Article] Essential role of Duox in stabilization of Drosophila wing (2011)
  • Author(s): N.T.T.Anh, M.Nishitani, S.Harada, M.Yamaguchi, K.Kamei
  • Journal Title: J.Biol.l Chem. Volume: Vol.286 Issue: 38 Pages: 33244-33251
  • DOI: 10.1074/jbc.m111.263178
  • Peer Reviewed
• [Journal Article] Neutralization of Toxic Heme by Porphyromonas gingivalis Hemoglobin Receptor. (2010)
  • Author(s): N.T.Nhien, et al.
  • Journal Title: J Biochem. Volume: 147 Pages: 317-325
  • Peer Reviewed
• [Journal Article] Structural analysis of glycogen-like polysaccharides having macrophage-activating activity in extracts of Lentinula edodes mycelia. (2010)
  • Author(s): Kojima H, et al.
  • Journal Title: J Nat Med(Tokyo) Volume: 64 Pages: 16-23
  • Peer Reviewed
• [Presentation] Essential role of Duox in stabilization of Drosophila wing. (2011)
  • Author(s): Nguyen Ti Tu Anh、西谷真依子、山口政光、亀井加恵子
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-12-13
• [Presentation] Essential role of Duox in stabilization of Drosophila wing (2011)
  • Author(s): Nguyen Ti Tu Anh, ら
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-13
• [Presentation] Role of Duox oxidase in wing development of Drosophila melanogaster (2010)
  • Author(s): Nguyen Ti Tu Anh、西谷真依子、山口政光、亀井加恵子
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-07
• [Presentation] Role of Duox oxidase in wing development of Drosophila melanogaster (2010)
  • Author(s): Nguyen Thi Tu Anh, et al.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸(神戸国際展示場)
  • Year and Date: 2010-12-07