Studies on a role of autophagy on the catabolism of sialylated oligosaccharides

• Project/Area Number: 22570148
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SUZUKI Tadashi 独立行政法人理化学研究所, 糖鎖代謝学研究チーム, チームリーダー (90345265)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 遊離糖鎖 / オートファジー / シアル酸 / 糖鎖代謝 / 細胞質 / リソソーム / 血清

Research Abstract

Previously, we showed that in Atg5^<-/-> cells, sialyl oligosaccharides specifically accumulated in the cytosol. Accumulation of these glycans was observed under non-starved conditions, suggesting that non-induced, basal autophagy is essential for their catabolism. Interestingly, once accumulated in the cytosol, sialyl glycans cannot be efficiently catabolized by resumption of the autophagic process, suggesting that functional autophagy is important for preventing sialyl oligosaccharides from accumulating in the cytosol. Moreover, knockdown of sialin, a lysosomal transporter of sialic acids, resulted in a significant reduction of sialyl oligosaccharides, implying that autophagy affects the substrate specificity of this transporter. This study thus provides a surprising link between basal autophagy and catabolism of N-linked glycans.

Research Products

• [Journal Article] free oligosaccharides: formation and degradation (free oligosaccharides related to N-linked glycans) (2013)
  • Author(s): Tadashi Suzuki
  • Journal Title: Encycl. Biol. Chem. (2^<nd> ed.) Volume: 2 Pages: 329-331
• [Journal Article] Free oligosaccharides: formation and degradation (free oligosaccharides related to N-linked glycans) (2013)
  • Author(s): Tadashi Suzuki
  • Journal Title: Encyclopedia of Biological Chemistry 2nd Editon Volume: 2 Pages: 329-331
• [Journal Article] Calystegine B3 as a specific inhibitor for cytoplasmic alpha-mannosidase, Man2C1 (2011)
  • Author(s): Atsushi Kato, Li Wang, Kumiko Ishii, Junichi Seino, Naoki Asano and Tadashi Suzuki
  • Journal Title: J. Biochem. Volume: 149 Pages: 415-422
  • Peer Reviewed
• [Journal Article] Metabolism of free oligosaccharides is facilitated in the och1 mutant of Saccharomyces cerevisiae (2011)
  • Author(s): Hiroto Hirayama and Tadashi Suzuki
  • Journal Title: Glycobiology Volume: 21 Pages: 1341-1348
  • Peer Reviewed
• [Journal Article] N-glycan is essential for stability and intracellular trafficking of glucose transporter, GLUT4 (2011)
  • Author(s): Yoshimi Haga, Kumiko Ishii and Tadashi Suzuki
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 31320-31327
  • Peer Reviewed
• [Journal Article] Calystegine B3 as a specific inhibitor for cytoplasmic α-mannosidase, Man2C1 (2011)
  • Author(s): A.Kato, L.Wang, K.Ishii, J.Seino, N.Asano, T.Suzuki
  • Journal Title: Journal of Biochemistry Volume: 149 Issue: 4 Pages: 415-422
  • DOI: 10.1093/jb/mvq153
  • Peer Reviewed
• [Journal Article] N-Glycosylation Is Critical for the Stability and Intracellular Trafficking of Glucose Transporter GLUT4 (2011)
  • Author(s): Yoshimi Haga, Kumiko Ishii, Tadashi Suzuki
  • Journal Title: The Journal of Biological Chemistry Volume: 286 Issue: 36 Pages: 31320-31327
  • DOI: 10.1074/jbc.m111.253955
  • Peer Reviewed
• [Presentation] N型糖鎖代謝とオートファジーの不思議な関係 (2012)
  • Author(s): 鈴木 匡
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡(国内学会講演)
  • Year and Date: 2012-12-15
• [Presentation] 糖鎖の一生を探る (2012)
  • Author(s): 鈴木 匡
  • Organizer: 第10回日本糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 東京(国内学会講演)
  • Year and Date: 2012-11-30
• [Presentation] The cytoplasmic peptide: N-glycanase (PNGase) and non-lysosomal catabolic pathway for N-glycans.(英語発表) (2011)
  • Author(s): 鈴木 匡
  • Organizer: 第34回日本分子生物学会大会
  • Place of Presentation: 横浜(国内学会講演)
  • Year and Date: 2011-12-16
• [Presentation] The cytoplasmic peptide : N-glycanase (PNGase) and non-lysosomal catabolic pathway for glycans (2011)
  • Author(s): T.Suzuki
  • Organizer: 第34回日本分子生物学会大会
  • Place of Presentation: 横浜(英語発表)
  • Year and Date: 2011-12-16
• [Presentation] N型遊離糖鎖の代謝におけるオートファジーの役割について (2011)
  • Author(s): 清野 淳一、王 麗、石井 久美子、水島 昇、鈴木 匡
  • Organizer: GlycoTOKYO 2011 シンポジウム
  • Place of Presentation: 和光(国内学会発表)
  • Year and Date: 2011-12-09
• [Presentation] N型遊離糖鎖の代謝におけるオートファジーの役割について (2011)
  • Author(s): 清野淳一、王麗、石井久美子、水島昇、鈴木匡
  • Organizer: GlycoTOKYO 2011シンポジウム
  • Place of Presentation: 和光
  • Year and Date: 2011-12-09
• [Presentation] Role of autophagy in the catabolism of sialylated oligosaccharides in mammalian cells (2011)
  • Author(s): Tadashi Suzuki
  • Organizer: 31^<st> Naito Conference on Glycan Expression and Regulation [II]
  • Place of Presentation: Sapporo (Japan)(国際学会講演)
  • Year and Date: 2011-09-14
• [Presentation] Role of autophagy in the catabolism of sialylated oligosaccharides in mammalian cells (2011)
  • Author(s): T.Suzuki
  • Organizer: 31^<st> Naito Conference on Glycan Expression and Regulation [II]
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-14
• [Presentation] A novel metabolic pathway for N-linked glycans (Keynote Lecture) (2011)
  • Author(s): Tadashi Suzuki
  • Organizer: 21st International Symposium on Glycoconjugates
  • Place of Presentation: Vienna (Austria)(国際学会講演)
  • Year and Date: 2011-08-25
• [Presentation] A novel metabolic pathway for N-linked glycans (2011)
  • Author(s): T.Suzuki
  • Organizer: 21^<th> International Symposium on Glycoconjugates
  • Place of Presentation: Vienna (Austria)(Keynote Lecture)
  • Year and Date: 2011-08-25
• [Presentation] Autophagy regulates catabolism of N-linked oligosaccharides (2011)
  • Author(s): Junichi Seino, Li Wang, Kumiko Ishii, Noboru Mizushima, and Tadashi Suzuki
  • Organizer: Glycobiology Gordon Research Conference
  • Place of Presentation: Barga (Italy)(国際学会講演)
  • Year and Date: 2011-05-09
• [Presentation] Autophagy regulates catabolism of sialylated oligosaccharides (2011)
  • Author(s): T.Suzuki
  • Organizer: Glycobiology Gordon Research Conference
  • Place of Presentation: Barga (Italy)
  • Year and Date: 2011-05-09
• [Presentation] Free oligosaccharides in the cytosol: formation and degradation.(英語発表) (2010)
  • Author(s): 鈴木 匡
  • Organizer: 第33回日本分子生物学会大会/第83回日本生化学会大会合同大会 (BMB2010)
  • Place of Presentation: 神戸(国内学会講演)
  • Year and Date: 2010-12-10
• [Presentation] Free oligosaccharides in the cytosol : formation and degradation. (2010)
  • Author(s): 鈴木匡
  • Organizer: 第33回日本分子生物学会大会/第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸(兵庫)
  • Year and Date: 2010-12-10
• [Presentation] Role of autophagy in the catabolism of sialylated N-glycans (2010)
  • Author(s): Junichi Seino, Li Wang, Kumiko Ishii, Noboru Mizushima, and Tadashi Suzuki
  • Organizer: Sialoglyco2010
  • Place of Presentation: Potsdam (Germany)(国際学会講演)
  • Year and Date: 2010-08-22
• [Presentation] Role of autophagy in the catabolism of sialylated N-glycans. (2010)
  • Author(s): J.Seino, K.Ishii, N.Mizushima, T.Suzuki(発表者:鈴木匡)
  • Organizer: Sialoglyco2010
  • Place of Presentation: Posdam, Germany
  • Year and Date: 2010-08-22
• [Presentation] Free oligosaccharides in the cytosol: formation and degradation (2010)
  • Author(s): Tadashi Suzuki
  • Organizer: International Symposium on Organelle Network: Interface among Infection-Immunity, Cell Biology and Glycobiology
  • Place of Presentation: Toyonaka (Japan)(国際学会講演)
  • Year and Date: 2010-04-13
• [Presentation] Free oligosaccharides in the cytosol : formation and degradation. (2010)
  • Author(s): 鈴木匡
  • Organizer: International Symsposium on organelle network : interface among infection-immunity, cell biology and glycobiology
  • Place of Presentation: 豊中(大阪)
  • Year and Date: 2010-04-13
• [Presentation] Free oligosaccharides: recent topics (2010)
  • Author(s): Tadashi Suzuki
  • Organizer: 2^<nd> Japan/China Joint Symposium on Glycobiology
  • Place of Presentation: Wako (Japan)(国際学会講演)
  • Year and Date: 2010-04-07
• [Presentation] Free oligosaccharides :recent topics. (2010)
  • Author(s): 鈴木匡
  • Organizer: 2^<nd> Japan/China Joint Symposium on Glycobiology
  • Place of Presentation: 和光(埼玉)
  • Year and Date: 2010-04-07
• [Presentation] N型糖鎖代謝とオートファジーの不思議な関係
  • Author(s): 鈴木 匡
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] 糖鎖の一生を探る
  • Author(s): 鈴木 匡
  • Organizer: 第10回糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 東京
  • Invited
• [Book] Chapter 6, mammalian sialidases In "Sialobiology; structure, biosynthesis and function of sialic acid glycoconjugates in health and disease (2013)
  • Author(s): Tadashi Suzuki and Katsunori Yamaguchi
  • Publisher: Bentham Science Publisher Ltd.
• [Book] Sialobiology; Structure, Biosynthesis and Function of Sialic Acid Glycoconjugates in Health and Disease (J. Tiralongo and I. Martinez-Duncker, eds.) (2013)
  • Author(s): Tadashi Suzuki and Katsunori Yamaguchi (Chapter 6, Mammalian Sialidases)
  • Total Pages: 531
  • Publisher: Bentham Science Publisher Ltd.
• [Remarks]
  • URL: http://www.riken.go.jp/research/labs/grc/riken_max_planck/sys_glycobiol/glycometabolome/
• [Remarks]
  • URL: http://www.riken.jp/r-world/research/lab/frontier-rs/sys-gly/glyco/index.html