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Studies on a role of autophagy on the catabolism of sialylated oligosaccharides

Research Project

Project/Area Number 22570148
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SUZUKI Tadashi  独立行政法人理化学研究所, 糖鎖代謝学研究チーム, チームリーダー (90345265)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords遊離糖鎖 / オートファジー / シアル酸 / 糖鎖代謝 / 細胞質 / リソソーム / 血清
Research Abstract

Previously, we showed that in Atg5^<-/-> cells, sialyl oligosaccharides specifically accumulated in the cytosol. Accumulation of these glycans was observed under non-starved conditions, suggesting that non-induced, basal autophagy is essential for their catabolism. Interestingly, once accumulated in the cytosol, sialyl glycans cannot be efficiently catabolized by resumption of the autophagic process, suggesting that functional autophagy is important for preventing sialyl oligosaccharides from accumulating in the cytosol. Moreover, knockdown of sialin, a lysosomal transporter of sialic acids, resulted in a significant reduction of sialyl oligosaccharides, implying that autophagy affects the substrate specificity of this transporter. This study thus provides a surprising link between basal autophagy and catabolism of N-linked glycans.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (33 results)

All 2013 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (22 results) (of which Invited: 2 results) Book (2 results) Remarks (2 results)

  • [Journal Article] free oligosaccharides: formation and degradation (free oligosaccharides related to N-linked glycans)2013

    • Author(s)
      Tadashi Suzuki
    • Journal Title

      Encycl. Biol. Chem. (2^<nd> ed.)

      Volume: 2 Pages: 329-331

    • Related Report
      2012 Final Research Report
  • [Journal Article] Free oligosaccharides: formation and degradation (free oligosaccharides related to N-linked glycans)2013

    • Author(s)
      Tadashi Suzuki
    • Journal Title

      Encyclopedia of Biological Chemistry 2nd Editon

      Volume: 2 Pages: 329-331

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Calystegine B3 as a specific inhibitor for cytoplasmic alpha-mannosidase, Man2C12011

    • Author(s)
      Atsushi Kato, Li Wang, Kumiko Ishii, Junichi Seino, Naoki Asano and Tadashi Suzuki
    • Journal Title

      J. Biochem.

      Volume: 149 Pages: 415-422

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metabolism of free oligosaccharides is facilitated in the och1 mutant of Saccharomyces cerevisiae2011

    • Author(s)
      Hiroto Hirayama and Tadashi Suzuki
    • Journal Title

      Glycobiology

      Volume: 21 Pages: 1341-1348

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] N-glycan is essential for stability and intracellular trafficking of glucose transporter, GLUT42011

    • Author(s)
      Yoshimi Haga, Kumiko Ishii and Tadashi Suzuki
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 31320-31327

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calystegine B3 as a specific inhibitor for cytoplasmic α-mannosidase, Man2C12011

    • Author(s)
      A.Kato, L.Wang, K.Ishii, J.Seino, N.Asano, T.Suzuki
    • Journal Title

      Journal of Biochemistry

      Volume: 149 Issue: 4 Pages: 415-422

    • DOI

      10.1093/jb/mvq153

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] N-Glycosylation Is Critical for the Stability and Intracellular Trafficking of Glucose Transporter GLUT42011

    • Author(s)
      Yoshimi Haga, Kumiko Ishii, Tadashi Suzuki
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 286 Issue: 36 Pages: 31320-31327

    • DOI

      10.1074/jbc.m111.253955

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] N型糖鎖代謝とオートファジーの不思議な関係2012

    • Author(s)
      鈴木 匡
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡(国内学会講演)
    • Year and Date
      2012-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] 糖鎖の一生を探る2012

    • Author(s)
      鈴木 匡
    • Organizer
      第10回日本糖鎖科学コンソーシアムシンポジウム
    • Place of Presentation
      東京(国内学会講演)
    • Year and Date
      2012-11-30
    • Related Report
      2012 Final Research Report
  • [Presentation] The cytoplasmic peptide: N-glycanase (PNGase) and non-lysosomal catabolic pathway for N-glycans.(英語発表)2011

    • Author(s)
      鈴木 匡
    • Organizer
      第34回日本分子生物学会大会
    • Place of Presentation
      横浜(国内学会講演)
    • Year and Date
      2011-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] The cytoplasmic peptide : N-glycanase (PNGase) and non-lysosomal catabolic pathway for glycans2011

    • Author(s)
      T.Suzuki
    • Organizer
      第34回日本分子生物学会大会
    • Place of Presentation
      横浜(英語発表)
    • Year and Date
      2011-12-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] N型遊離糖鎖の代謝におけるオートファジーの役割について2011

    • Author(s)
      清野 淳一、王 麗、石井 久美子、水島 昇、鈴木 匡
    • Organizer
      GlycoTOKYO 2011 シンポジウム
    • Place of Presentation
      和光(国内学会発表)
    • Year and Date
      2011-12-09
    • Related Report
      2012 Final Research Report
  • [Presentation] N型遊離糖鎖の代謝におけるオートファジーの役割について2011

    • Author(s)
      清野淳一、王麗、石井久美子、水島昇、鈴木匡
    • Organizer
      GlycoTOKYO 2011シンポジウム
    • Place of Presentation
      和光
    • Year and Date
      2011-12-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Role of autophagy in the catabolism of sialylated oligosaccharides in mammalian cells2011

    • Author(s)
      Tadashi Suzuki
    • Organizer
      31^<st> Naito Conference on Glycan Expression and Regulation [II]
    • Place of Presentation
      Sapporo (Japan)(国際学会講演)
    • Year and Date
      2011-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Role of autophagy in the catabolism of sialylated oligosaccharides in mammalian cells2011

    • Author(s)
      T.Suzuki
    • Organizer
      31^<st> Naito Conference on Glycan Expression and Regulation [II]
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] A novel metabolic pathway for N-linked glycans (Keynote Lecture)2011

    • Author(s)
      Tadashi Suzuki
    • Organizer
      21st International Symposium on Glycoconjugates
    • Place of Presentation
      Vienna (Austria)(国際学会講演)
    • Year and Date
      2011-08-25
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel metabolic pathway for N-linked glycans2011

    • Author(s)
      T.Suzuki
    • Organizer
      21^<th> International Symposium on Glycoconjugates
    • Place of Presentation
      Vienna (Austria)(Keynote Lecture)
    • Year and Date
      2011-08-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Autophagy regulates catabolism of N-linked oligosaccharides2011

    • Author(s)
      Junichi Seino, Li Wang, Kumiko Ishii, Noboru Mizushima, and Tadashi Suzuki
    • Organizer
      Glycobiology Gordon Research Conference
    • Place of Presentation
      Barga (Italy)(国際学会講演)
    • Year and Date
      2011-05-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Autophagy regulates catabolism of sialylated oligosaccharides2011

    • Author(s)
      T.Suzuki
    • Organizer
      Glycobiology Gordon Research Conference
    • Place of Presentation
      Barga (Italy)
    • Year and Date
      2011-05-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Free oligosaccharides in the cytosol: formation and degradation.(英語発表)2010

    • Author(s)
      鈴木 匡
    • Organizer
      第33回日本分子生物学会大会/第83回日本生化学会大会合同大会 (BMB2010)
    • Place of Presentation
      神戸(国内学会講演)
    • Year and Date
      2010-12-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Free oligosaccharides in the cytosol : formation and degradation.2010

    • Author(s)
      鈴木匡
    • Organizer
      第33回日本分子生物学会大会/第83回日本生化学会大会合同大会(BMB2010)
    • Place of Presentation
      神戸(兵庫)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Role of autophagy in the catabolism of sialylated N-glycans2010

    • Author(s)
      Junichi Seino, Li Wang, Kumiko Ishii, Noboru Mizushima, and Tadashi Suzuki
    • Organizer
      Sialoglyco2010
    • Place of Presentation
      Potsdam (Germany)(国際学会講演)
    • Year and Date
      2010-08-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Role of autophagy in the catabolism of sialylated N-glycans.2010

    • Author(s)
      J.Seino, K.Ishii, N.Mizushima, T.Suzuki(発表者:鈴木匡)
    • Organizer
      Sialoglyco2010
    • Place of Presentation
      Posdam, Germany
    • Year and Date
      2010-08-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Free oligosaccharides in the cytosol: formation and degradation2010

    • Author(s)
      Tadashi Suzuki
    • Organizer
      International Symposium on Organelle Network: Interface among Infection-Immunity, Cell Biology and Glycobiology
    • Place of Presentation
      Toyonaka (Japan)(国際学会講演)
    • Year and Date
      2010-04-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Free oligosaccharides in the cytosol : formation and degradation.2010

    • Author(s)
      鈴木匡
    • Organizer
      International Symsposium on organelle network : interface among infection-immunity, cell biology and glycobiology
    • Place of Presentation
      豊中(大阪)
    • Year and Date
      2010-04-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] Free oligosaccharides: recent topics2010

    • Author(s)
      Tadashi Suzuki
    • Organizer
      2^<nd> Japan/China Joint Symposium on Glycobiology
    • Place of Presentation
      Wako (Japan)(国際学会講演)
    • Year and Date
      2010-04-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Free oligosaccharides :recent topics.2010

    • Author(s)
      鈴木匡
    • Organizer
      2^<nd> Japan/China Joint Symposium on Glycobiology
    • Place of Presentation
      和光(埼玉)
    • Year and Date
      2010-04-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] N型糖鎖代謝とオートファジーの不思議な関係

    • Author(s)
      鈴木 匡
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 糖鎖の一生を探る

    • Author(s)
      鈴木 匡
    • Organizer
      第10回糖鎖科学コンソーシアムシンポジウム
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] Chapter 6, mammalian sialidases In "Sialobiology; structure, biosynthesis and function of sialic acid glycoconjugates in health and disease2013

    • Author(s)
      Tadashi Suzuki and Katsunori Yamaguchi
    • Publisher
      Bentham Science Publisher Ltd.
    • Related Report
      2012 Final Research Report
  • [Book] Sialobiology; Structure, Biosynthesis and Function of Sialic Acid Glycoconjugates in Health and Disease (J. Tiralongo and I. Martinez-Duncker, eds.)2013

    • Author(s)
      Tadashi Suzuki and Katsunori Yamaguchi (Chapter 6, Mammalian Sialidases)
    • Total Pages
      531
    • Publisher
      Bentham Science Publisher Ltd.
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.riken.go.jp/research/labs/grc/riken_max_planck/sys_glycobiol/glycometabolome/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.riken.jp/r-world/research/lab/frontier-rs/sys-gly/glyco/index.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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