Roles of heparan sulfate glycosaminoglycan containing positive charged domain on cell proliferation, differentiation and apoptosis

• Project/Area Number: 22570150
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Nihon Pharmaceutical University (2011-2012); Aichi Cancer Center Research Institute (2010)
• Principal Investigator: KYOGASHIMA Mamoru 日本薬科大学, 薬学部, 教授 (50225091)
• Co-Investigator(Renkei-kenkyūsha): EHARA Takashi 松本大学, 人間健康学部, 教授 (00203646) ; ODA Koji 愛知県がんセンター(研究所), 疫学・予防部, 研究員 (30311715)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 糖鎖生物学 / ヘパラン硫酸糖鎖 / 陽性荷電 / ヘパラン硫酸 / 細胞増殖 / JM403抗原 / がんマーカー / 上皮 / 細胞分化 / 分化 / アポトーシス / がんバイオマーカー

Research Abstract

Expression of heparan sulfate glycosaminoglycan with positively charged domain (JM403 antigen) in cytoplasm was examined in proliferative and/or differentiated cells of cancers and fetal rodent tissues. The JM403 antigen was limitedly expressed in epithelial cells but not in nervous cells and connective tissue cells, suggesting its involvement with epithelial proliferation and differentiation.

Research Products

• [Journal Article] JM403 antigen GlcA-GlcNH3+ on heparan sulfate glycosaminoglycan may be involved in cell proliferation and apoptosis (2011)
  • Author(s): Akiko Yusa, et al
  • Journal Title: Glycoconjugate Journal Volume: 28 Pages: 270-271
• [Journal Article] Cytoplasmic expression of the JM403 antigen GlcA-GlcNH3+ on heparan sulfate glycosaminoglycan in mammary carcinomas - A novel proliferative biomarker for breast cancers with high malignancy (2010)
  • Author(s): Fujii M, Yusa A, Yokoyama Y, Kokuryo T, Tsunoda N, Oda K, Nagino M, Ishimaru T, Shimoyama Y, Utsunomiya H, Iwata H, Itoh Y, Itoh J, Kannagi R, Kyogashima M
  • Journal Title: Glycoconj J Volume: 27 Issue: 7-9 Pages: 661-672
  • DOI: 10.1007/s10719-010-9311-4
  • Peer Reviewed
• [Journal Article] Cytoplasmic expression of the JM403 antigen GlcA-GlcNH_3^+ on heparan sulfate glycosaminoglycan in mammary carcinomas-A novel proliferative biomarker for breast cancers with high malignancy. (2010)
  • Author(s): Fujii M, et al.
  • Journal Title: Glycoconjugate Journal Volume: 27 Pages: 661-672
  • Peer Reviewed
• [Presentation] JM403 antigen GlcA-GlcNH3+on heparan sulfate glycosaminoglycan may be involved in cell proliferation and apoptosis (2011)
  • Author(s): Akiko Yusa, Masahiro Fujii, Hirotoshi Utsunomiya, Kouji Tanaka, Reiji Kannagi, Koji Oda, Mamoru Kyogashima
  • Organizer: XXI International Symposium on Glycoconjugates
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-08-22
• [Presentation] JM403 antigen GlcA-GlcNH3+ on heparan sulfate glycosaminoglycan may involve in cell proliferation, and apoptosis (2011)
  • Author(s): Akiko Yusa, et al
  • Organizer: 21^<st> International Symposium on Glycoconjugates
  • Place of Presentation: オーストリア・ウィーン
  • Year and Date: 2011-08-22
• [Presentation] Different expression of cationic heparan sulfate in the cells at proliferation, differentiation and apoptosis (2010)
  • Author(s): Akiko Yusa, et al.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] 陽性荷電をもつヘパラン硫酸糖 鎖GlcA-GlcNH3+の細胞生物学的研究 (2010)
  • Author(s): 遊佐亜希子、藤井正宏、後藤嘉子、小田高司、宇都宮洋才、京ケ島 守、神奈木玲児
  • Organizer: 糖鎖科学名古屋拠点「若手の力」フォーラム第8回
  • Place of Presentation: 名古屋
  • Year and Date: 2010-09-06
• [Presentation] Different expression of cationic heparan sulfate in the cells at proliferation (2010)
  • Author(s): Akiko Yusa, Masahiro Fujii, Yoshiko Goto, Koji Oda, Hirotoshi Utsunomiya, Mamoru Kyogashima, Reiji Kannagi
  • Organizer: differentiation and apoptosis.BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-01-20