| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
Effects of low-dose bisphenol A (BPA) on the hippocampus were examined. (1)In adult rat hippocampal slices, 10 nM BPA increased dendritic spines (especially middle-head spines with head diameter of 0.4-0.5 μm) within 2h via ERRγ localized in principal neurons. Inhibitors of MAP kinases and NMDA receptors blocked the BPA effects. (2)Perinatal exposure to low-dose BPA (30 μg/kg BW/day) via dam modulated the synapse development in the pup hippocampus. In male at postnatal8-weeks, the spine density was decreased compared with that of control. In female, perinatal BPA exposure attenuated the estrus cycle-dependent spine density changes, resulting in the disappearance of sex-related differences. (3)BPA concentration was determined in male hippocampus (approx. 64 nM) by mass-spectrometry.
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