Molecular mechanism of a light-regulated transcription factor,aureochrome.
Project/Area Number |
22570162
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biophysics
|
Research Institution | Osaka University |
Principal Investigator |
HISATOMI Osamu 大阪大学, 大学院・理学研究科, 准教授 (60231544)
|
Co-Investigator(Renkei-kenkyūsha) |
KATAOKA Hironao 東北大学, 情報公開センター, 研究員 (30108568)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
Keywords | 光制御転写因子 / LOVドメイン / DNA結合 / 光スイッチ / 転写因子 / ナノバイオ / 分子認識 / 生物物理 / bZIPドメイン / DNA結合タンパク質 / 光形態形成 |
Research Abstract |
To understand the molecular mechanism of a blue light (BL)-regulated transcription factor,aureochrome (AUREO), we prepared six kinds of recombinant AUREOs. BL appeared to induce a shift of the α-helical structure of the LOV domain to a β-sheet structure, and an approximately 5% increase in the hydrodynamic radius of recombinant AUREOs. Our results support a schema where BL-induced changes in the LOV domain may cause global conformational changes in the bZIP and/or the linker of AUREO.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Blue light-induced conformational changes in a light-regulated transcription factor, aureochrome-1.2013
Author(s)
Hisatomi, O., Takeuchi, K., Zikihara, K., Ookubo, Y., Nakatani, Y., Takahashi, F., Tokutomi, S., Kataoka, H.
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Journal Title
Plant Cell Physiol.
Volume: 54(1)
Pages: 93-106
NAID
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[Presentation] Photoreaction of a light-regulated transcription factor, AUREO12011
Author(s)
Hisatomi, O., Takeuchi, K., Murakami, T.,Takahashi, F., Kataoka, H.
Organizer
日本生物物理学会第49回年会
Place of Presentation
兵庫県立大学(兵庫県)
Year and Date
2011-09-18
Related Report
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[Presentation] Characterization of a light-regulated transcription factor, AUREO1, expressed in E. coli2011
Author(s)
Hisatomi, O., Murakami, T., Furuya, K., Takahashi, F. and Kataoka, H
Organizer
5th Asia and Oceania Conference on Photobiology (AOCP2011)
Place of Presentation
Nara Prefectural New Public Hall, Nara, Japan
Year and Date
2011-07-31
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[Presentation] Blue-light induced conformational change of a transcription factor, AUREO1.2011
Author(s)
Hisatomi, O., Takeuchi, K., Nakatani, Y., Takahashi, F., Kataoka, H
Organizer
日本生物物理学会第50回年会
Place of Presentation
名古屋大学(東山キャンパス)
Related Report
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[Presentation] FTIR study of Aureochrome that possesses LOV domain at the C-terminus.2011
Author(s)
Ito, S., Zhang, Yu., Iwata, T., Hisatomi, O., Takahashi, F., Kataoka, H., Kandori, H
Organizer
日本生物物理学会第50回年会
Place of Presentation
名古屋大学(東山キャンパス)
Related Report
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[Presentation] Analyses of a light-regulated transcription factor, AUREO1, expressed in E.coli2010
Author(s)
Hisatomi, O., Ohata, R., Furuya, K., Takahashi, F., Kataoka H
Organizer
日本生物物理学会第48回年会
Place of Presentation
東北大学(仙台市)
Year and Date
2010-09-20
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[Presentation] Analyses of a light-regulated transcription factor, AUREO1, expressed in E.coli.2010
Author(s)
Hisatomi, O., Ohata, R., Furuya, K., Takahashi, F., Kataoka, H
Organizer
日本生物物理学会第48回年会
Place of Presentation
東北大(川内キャンパス)
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