Analysis of the recognition of stop codons by eRF1 using various species of ciliates
| Project/Area Number |
22570170
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Nara Women's University |
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Principal Investigator |
HARUMOTO Terue 奈良女子大学, 自然科学系, 教授 (80198936)
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| Co-Investigator(Kenkyū-buntansha) |
SUZAKI Toshinobu 神戸大学, 大学院・理学研究科, 准教授 (00187692)
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| Co-Investigator(Renkei-kenkyūsha) |
ITO Koichi 東京大学, 大学院・新領域創成科学研究科, 准教授 (10262073)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | タンパク質合成 / 遺伝暗号 / 原生生物 / 翻訳終結因子 / 翻訳 / コドン / 繊毛虫 / eRF1 |
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| Research Abstract |
To identify key residues in ciliate eRF1s for stop codon recognition, we introduced mutations at the predicted sites of Dileptus eRF1 and examined the activity by in vivo yeast assay systems. The results indicate that R128 is one of the key residues preserving the ability to recognize all three stop codons, especially UGA in ciliate eRF1s.
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Report
(4 results)
Research Products
(114 results)
