Analysis of the mechanism of histone methylation by using genetic approaches

• Project/Area Number: 22570176
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HAYASHI Aki 独立行政法人理化学研究所, クロマチン動態研究チーム, 研究員 (80399534)
• Co-Investigator(Renkei-kenkyūsha): NAKAYAMA Jun-ichi 名古屋市立大学, システム自然科学研究科, 准教授 (60373338)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ヘテロクロマチン / RNAi機構 / ヒストン修飾 / HP1

Research Abstract

Epigenetics plays an important role for regulation of gene expression during developmental stages in eukaryote. To understand the molecular mechanism of epigenetic regulation, I analyzed the mechanism of histone modification at heterochromatic region of fission yeast by using genetic approaches. I obtained two uncharacterized genes involved in the formation of heterochromatin by screening. One of the genes, Ers1, binds both of heterochromatin protein 1 (HP1) and an RNA helicase Hrr1 functioned in RNAi pathway. Genetic and biochemical analysis revealed that Ers1 recruits the RNAi machinery to heterochromatic region and facilitates siRNA generation through the binding of HP1.

Research Products

• [Journal Article] Heterochromatin protein 1 homologue Swi6 acts in concert with Ers1 to regulate RNAi-directed heterochromatin assembly (2012)
  • Author(s): Hayashi A, Ishida M, Kawaguchi R, Urano T, Murakami Y, Nakayama J.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 109 Pages: 6159-6164
  • Peer Reviewed
• [Journal Article] A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA (2012)
  • Author(s): Kawakami K, Hayashi A, Nakayama J, Murakami Y.
  • Journal Title: Genes Dev. Volume: 26 Pages: 1811-24
  • Peer Reviewed
• [Journal Article] Intrinsic nucleic acid-binding activity of Chp1 chromodomain is required for heterochromatin assembly (2012)
  • Author(s): 3.Ishida M, Shimojo H, Hayashi A, Kawaguchi R, Ohtani Y, Uegaki K, Nishimura Y, Nakayama J.
  • Journal Title: Mol Cell. Volume: 47 Pages: 1-14
  • Peer Reviewed
• [Journal Article] A novel RNAi protein, Dsh1, assembles RNAi machinery on chromatin to amplify heterochromatic siRNA. (2012)
  • Author(s): Kei Kawakami
  • Journal Title: Genes Dev. Volume: 26 Pages: 1811-1824
  • Peer Reviewed
• [Presentation] Ers1因子はHP1を介してヘテロクロマチン上にRNAi機構をリクルートする (2012)
  • Author(s): 林 亜紀
  • Organizer: 日本遺伝学会第84回大会
  • Place of Presentation: 博多(口頭)
• [Presentation] Ers1はHP1を介してRNAi機構をヘテロクロマチンにリクルートする (2012)
  • Author(s): 林 亜紀
  • Organizer: 第45回 酵母遺伝学フォーラム
  • Place of Presentation: 宇治(口頭)
• [Presentation] HP1/Swi6 Acts in Concert with Ers1 to Regulate RNAi-directed Heterochromatin Assembly (2012)
  • Author(s): Aki Hayashi
  • Organizer: 22^<nd> CDB meeting: RNA Sciences in Cell and Developmental Biology II
  • Place of Presentation: Kobe(oral)
• [Presentation] Ers1因子はHP1を介してヘテロクロマチン上にRNAi機構をリクルートする (2012)
  • Author(s): 林 亜紀
  • Organizer: 日本遺伝学会 第８４回大会
  • Place of Presentation: 九大百年講堂・博多
• [Presentation] Ers1はHP1を介してRNAi機構をヘテロクロマチンにリクルートする (2012)
  • Author(s): 林 亜紀
  • Organizer: 第４５回 酵母遺伝学フォーラム
  • Place of Presentation: きはだホール・宇治
• [Presentation] HP1/Swi6 Acts in Concert with Ers1 to Regulate RNAi-directed Heterochromatin Assembly (2012)
  • Author(s): Aki Hayashi
  • Organizer: 22nd CDB meeting: RNA Sciences in Cell and Developmental Biology II
  • Place of Presentation: CDB, Kobe
• [Presentation] HP1/Swi6はErs1を介してRNAi機構によるヘテロクロマチン形成を促進する (2011)
  • Author(s): 林亜紀
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-15
• [Presentation] Ers1 links heterochromatin and RNAi machinery (2011)
  • Author(s): Aki Hayashi
  • Organizer: The Sixth International Fission Yeast Meeting
  • Place of Presentation: Harvard Medical School (Boston, U.S.A.,)
  • Year and Date: 2011-06-26
• [Presentation] 分裂酵母ヘテロクロマチン形成とRNAi機構のリンク (2011)
  • Author(s): 林亜紀・石田真由美・中山潤一
  • Organizer: 第28回染色体ワークショップ
  • Place of Presentation: 瑠璃光・石川県加賀市
  • Year and Date: 2011-01-13
• [Presentation] HP1/Swi6 acts in concert with Ers1 to drive RNAi-directed heterochromatin assembly (2011)
  • Author(s): 林 亜紀
  • Organizer: 第34回 日本分子生物学会
  • Place of Presentation: 横浜(口頭+ポスター)
• [Presentation] Ers1 links heterochromatin and RNAi machinery (2011)
  • Author(s): Aki Hayashi.
  • Organizer: 6th International Fission Yeast Meeting
  • Place of Presentation: Boston, U.S.A.(poster)
• [Presentation] 分裂酵母ヘテロクロマチン形成とRNAi機構のリンク (2011)
  • Author(s): 林亜紀
  • Organizer: 第28回 染色体ワークショップ
  • Place of Presentation: 山代(口頭)