Molecular mechanism of microtubule stabilization by plus-end tracking proteins

• Project/Area Number: 22570190
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Yokohama City University
• Principal Investigator: HAYASHI Ikuko 横浜市立大学, 大学院・生命医科学研究科, 准教授 (80464527)
• Co-Investigator(Renkei-kenkyūsha): YASUNAGA Takuo 九州工業大学, 大学院・情報工学研究院, 教授 (60251394)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: x線結晶構造解析 / 電子顕微鏡解析 / 細胞骨格 / 細胞遊走 / ｘ線結晶構造解析

Research Abstract

Microtubule plus-endtracking protein CLASP proteins playa critical role in chromosomal segregation and cell migration. We determined the crystal structures of two microtubule-binding TOG domains in CLASP2. By mutational analysis, we showed that bothTOG domains are essential for CLASP2 binding to microtubules and are able to stabilizemicrotubules to a maximum extent when CLASP2 forms a complex with another microtubule-binding protein EB1.

Research Products

• [Journal Article] Crystallization and preliminary X-ray data analysis of the pXO1 plasmid-partitioning factor TubZ from Bacillus cereus (2012)
  • Author(s): Hoshino S, Maki T, Hayashi I
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun Volume: 68 Pages: 1550-3
• [Journal Article] Filament formation of FtsZ/tubulin-like protein TubZ from the Bacillus cereus pXO1 plasmid (2012)
  • Author(s): Hoshino S, Hayashi I
  • Journal Title: J Biol Chem Volume: 287 Pages: 32103-12
• [Journal Article] A mutation of the fission yeast EB1 overcomes negative regulation by phosphorylation and stabilizes microtubules (2012)
  • Author(s): Iimori M, Ozaki K, Chikashige Y, Habu T, Hiraoka Y, Maki T, Hayashi I, Obuse C, Matsumoto T
  • Journal Title: Exp Cell Res Volume: 318 Pages: 262-75
  • NAID: 120003874262
• [Journal Article] Crystallization and preliminary X-ray data analysis of the pXO1 plasmid-partitioning factor TubZ from Bacillus cereus. (2012)
  • Author(s): S Hoshino, T Maki, I Hayashi
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun. Volume: 68 Issue: 12 Pages: 1550-1553
  • DOI: 10.1107/s1744309112045551
  • Peer Reviewed
• [Journal Article] Filament formation of FtsZ/tubulin-like protein TubZ from the Bacillus cereus pXO1 plasmid. (2012)
  • Author(s): S Hoshino, I Hayashi
  • Journal Title: J Biol Chem Volume: 287 Issue: 38 Pages: 32103-32112
  • DOI: 10.1074/jbc.m112.373803
  • Peer Reviewed
• [Presentation] Bacillus cereus由来pXO1様プラスミド分配因子TubZの結晶構造解析 (2012)
  • Author(s): 星野昌太、林郁子
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2012-12-16
• [Presentation] Structural analyses of pXO1 plasmid partitioning factor TubZ from Bacillus cereus (2012)
  • Author(s): S Hoshino, I Hayashi
  • Organizer: Gordon Research Conference
  • Place of Presentation: 米国ニューハンプシャー
  • Year and Date: 2012-07-19
• [Presentation] Molecular mechanism of cooperative microtubule stabilization by microtubule plus-end tracking proteins CLASP2 and EB1 (2012)
  • Author(s): T Maki,I Hayashi
  • Organizer: Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: 米国サンフランシスコ
• [Presentation] A Novel Microtubule Binding Protein, MARKAP1, Regulates Stability of the Golgi-NucleatedMicrotubules and Directional Cell Migration (2012)
  • Author(s): Y. Satoh, K. Hayashi, Y. Amano, M. Takahashi,I. Hayashi, S. Ohno, A. Suzuki
  • Organizer: Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: 米国サンフランシスコ
• [Presentation] Molecular mechanism of cooperative microtubule stabilization by microtubule plus-end tracking proteins CLASP2 and EB1 complex (2011)
  • Author(s): T Maki, I Hayashi
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] 新規微小管結合タンパク質p250による細胞運動極性制御機構の解析 (2011)
  • Author(s): Y Satoh, M Akitsu, K Hayashi, Y Amano, I Hayashi, S Ohno, Atsushi Suzuki
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Bacillus cereus 由来pXO1様プラスミド分配因子TubZの結晶構造解析 (2011)
  • Author(s): S Hoshino,I Hayashi
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] Structural analyses of pXO1 plasmid partitioning factor TubZ from Bacillus cereus (2011)
  • Author(s): S Hoshino,I Hayashi
  • Organizer: Gordon Research Conference
  • Place of Presentation: 米国ニューハンプシャー
• [Remarks]
  • URL: http://www.tsurumi.yokohama-cu.ac.jp/mmb/index.html
• [Remarks]
  • URL: http://www.tsurumi.yokohama-cu.ac.jp/biophys/index.html
• [Remarks]
  • URL: http://www.tsurumi.yokohama-cu.ac.jp/biophys/index.html