Molecular mechanism of microtubule stabilization by plus-end tracking proteins
Project/Area Number |
22570190
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Cell biology
|
Research Institution | Yokohama City University |
Principal Investigator |
HAYASHI Ikuko 横浜市立大学, 大学院・生命医科学研究科, 准教授 (80464527)
|
Co-Investigator(Renkei-kenkyūsha) |
YASUNAGA Takuo 九州工業大学, 大学院・情報工学研究院, 教授 (60251394)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | x線結晶構造解析 / 電子顕微鏡解析 / 細胞骨格 / 細胞遊走 / x線結晶構造解析 |
Research Abstract |
Microtubule plus-endtracking protein CLASP proteins playa critical role in chromosomal segregation and cell migration. We determined the crystal structures of two microtubule-binding TOG domains in CLASP2. By mutational analysis, we showed that bothTOG domains are essential for CLASP2 binding to microtubules and are able to stabilizemicrotubules to a maximum extent when CLASP2 forms a complex with another microtubule-binding protein EB1.
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Report
(4 results)
Research Products
(16 results)