The molecular analysis of the development of cerebellum by using zebrafish mutants
Project/Area Number |
22570201
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Developmental biology
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Research Institution | Nagoya University |
Principal Investigator |
SHIMIZU Takashi 名古屋大学, 生物機能開発利用研究センター, 准教授 (50324760)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 細胞分化 / 神経分化 / 小脳 / 顆粒細胞 / 神経発生 / 神経回路 / ゼブラフィッシュ |
Research Abstract |
To understand the genes that control the development of cerebellar neurons, we investigated the zebrafish mutants gazami(gaz) and shiomaneki(sio).The gaz mutant larvae showed a strong reduction of granule cells. By the positional cloning, we mapped the gaz mutation to the gene encoding a nuclear protein, cranio facial development protein1(cfdp1). In the anterior neuroectoderm, the number of apoptotic cells was increased in the mutant larvae. We also found that proliferating cells were increased in mutants at 4 dpf. Our findings suggest that Cfdp1 function in the differentiation from the neural progenitors and/or proliferation of neural progenitor cells.The sio mutants displayed mistargeted axon of granule cells. The sio mutation mapped to the gene encodes type IV collagen a6 whish serve as a component of basement membrane.The sio larvae showed the disruption of basement membrane structure. These results suggest that the interaction between neuron and basement membrane is important for the axogenesis of cerebellar granule cells.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Zinc finger genes Fezf1 and Fezf2 control neuronal differentiation by repressing Hes5 expression in forebrain2010
Author(s)
Shimizu, T., Nakazawa, M., Kani, S., Bae, Y.-K., Shimizu, T., Kageyama, R., and Hibi, M.
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Journal Title
Development
Volume: 137
Pages: 1875-1885
Related Report
Peer Reviewed
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[Journal Article] a motor protein linker, controls dorsal determination.2010
Author(s)
Nojima, H., Rothhamel, S., Shimizu, T., Kim, C.H., Yonemura, S., Marlow,F.L., and Hibi, M. Syntabulin
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Journal Title
Development
Volume: 137
Pages: 923-933
Related Report
Peer Reviewed
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[Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum2010
Author(s)
Kani, S., Bae, Y.-K., Shimizu, T., Tanabe, K., Satou, C, Parsons, M.J.Scott, E., Higashijima, S.-I., Hibi, M.
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Journal Title
Developmental Biology
Volume: 343
Pages: 1-17
Related Report
Peer Reviewed
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[Journal Article] Atypical PKC regulates primary dendrite specification of cerebellar Purkinje cells by Localizing Golgi apparatus2010
Author(s)
Tanabe, K., Kani, S., Shimizu, T., Bae, Y.-K., Abe, T., Hibi, M.
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Journal Title
J.Neurosci.
Volume: 30
Pages: 16983-16992
Related Report
Peer Reviewed
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[Presentation] Role of basement membrane in axogenesis of cerebellar granule cells in zebrafish2012
Author(s)
Hibi, M., Takeuchi, M., Yonemura, S., Asakawa, K., Kawakami, K., and Shimizu, T.
Organizer
Asia-Pacific Developmental Biology Conference 2012
Place of Presentation
台北、台湾
Related Report
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[Presentation] Role of basement membrane in axogenesis of cerebellar granule cells2012
Author(s)
Takeuchi, M., Yamaguchi, S., Yonemura, S, Asakawa, K., Kawakami, K., Takeda, S., Shimizu, T., and Hibi, M.
Organizer
第18回小型魚類研究会
Place of Presentation
京都
Related Report
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[Presentation] Role of type IV collagen in axogenesis of cerebellar granule cells in zebrafish2012
Author(s)
Takeuchi, M., Shimizu, T., Asakawa, K., Kawakami, K., Yonemura, S., and Hibi, M.
Organizer
10th International Conference on Zebrafish Development and Genetics.
Place of Presentation
Madison, USA
Related Report
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[Presentation] Role of type IV collagen in axogenesis of cerebellar granule cells in zebrafish2012
Author(s)
Takeuchi, M., Shimizu, T., Asakawa, K., Kawakami, K., Yonemura, S., and Hibi, M.
Organizer
第45回日本発生生物学会合同大会
Place of Presentation
神戸
Related Report
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[Presentation] Genetic control for development of cerebellar neurons and neural circuits in zebrafish2011
Author(s)
Takeuchi, M., Shimizu, T., Kani, S., Bae, Y.-K., Tanabe, K., Kusuda, R.*, Asakawa, K., Kawakami, K., and Hibi, M.
Organizer
第17回小型魚類研究会
Place of Presentation
三島
Related Report
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[Presentation] Genetic control of development of cerebellar neurons and neural circuits in zebrafish2011
Author(s)
Shimizu, T., Kani, S., Bae, Y.-K.,Tanabe, K., Kusuda, R., and Hibi, M.
Organizer
第44回日本発生生物学会年会
Place of Presentation
沖縄
Related Report
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[Presentation] Neurogenesis in Zebrafish Cerebellum2010
Author(s)
Shimizu, T., Kani, S., Bae, Y.-K., Tanabe, K., and Hibi, M.
Organizer
8th International Conference on Zebrfish Development and Genetics
Place of Presentation
Madison, USA
Related Report
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