| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Research Abstract |
The objective is to identify the role of Sonic hedgehog (Shh) during mammalian cortical development. We identified the source of Shh protein during cortical development using EGFP expression under Shh-creERT2/beta-geo-EGFP (ZEG) system under the administration of tamoxifen at both E13.5 and E14.5. Shh expressing cells were limited population of post-mitotic cortical neuronal cells in the intermedate zone. To identify the role of these cells, we induced apoptosis in the Shh expressing cells by using mice with Shh-creERT2/loxP-STOP-loxP-DTA background. By the ablation of the Shh-expressing cells, we detected significant reduction of the differentiation from neural stem cells, indicating that the Shh-expressing cells in the intermediate zone is required for the differentiation of neural stem cells into the mitotic progenitors.
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