Detections of genes and proteins related proanthocyanidins synthesis from catechins

• Project/Area Number: 22580011
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: MATSUI Katsuhiro 独立行政法人農業・食品産業技術総合研究機構, 九州沖縄農業研究センター・作物開発・利用研究領域, 主任研究員 (30343974)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 植物育種 / 遺伝 / プロアントシアニジン / MYB転写因子 / CRES-T / 二次代謝産物 / 形質転換体 / フラボノイド / トランジエントアッセイ / 自殖性ソバ / 転写因子 / MYB / 分離集団

Research Abstract

In this study, I had clarified that a gene of MYB transcription factor which was isolated from buckwheat was related to PA synthesis and regulations by production of transgenic plants. I also tried to detect genes and proteins relating synthesis PA by comparison of wild type and transgenic plants with cDNA-AFLP. I didn't find any specific genes by the way, and now I am preparing to do gel-free proteomic analysis to find them.

Research Products

• [Journal Article] QTL analysis of photoperiod sensitivity in common buckwheat by using markers for expressed sequence tags and photoperiod-sensitivity candidate genes (2011)
  • Author(s): Takashi Hara, Hiroyoshi Iwata, Kazutoshi Okuno, Katsuhiro Matsui, Ryo Ohsawa
  • Journal Title: Breeding Science Volume: 61 Pages: 394-404
  • NAID: 10030024366
  • Peer Reviewed
• [Presentation] Apple R2R3 MYB transcription factor, MdMYB2, acts as a repressor of anthocyanin biosynthesis (2012)
  • Author(s): Matsui K, Takos, A.M., Parker J.L., Robinson, S.P. and Walker, A.R.
  • Organizer: JAPAN-AUSTRALIA SYMPOSIUM ON PLANT SCIENCES FOR AGRICULTURE
  • Place of Presentation: 北海道大学農学部
  • Year and Date: 2012-01-20