Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Research Abstract |
In this study, we have cloned the rice blast partial resistance gene Pi34 and comparedthe cytological reaction carrying Pi34 and complete resistance genes. RNAi and inoculation assay confirmed that OMG02 was Pi34. OMG02 was predicted as the gene of unknown function with two exons. Responses of rice sheath epidermal cells carrying Pi34 to rice blast infection were similar to those carrying a complete resistance gene. The other hand, the reactive oxygen species (ROS) accumulation in infected leaf blades epidermal cells of NILs carrying either Pi34 or a complete resistance gene indicated that manners of ROS accumulation were similar to each other in 24 hours post inoculation (hpi) but differed in 48hpi.
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