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Characterization of the rice blast partial resistance using comparative analysis with the complete resistance

Research Project

Project/Area Number 22580055
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

KITO Hideki  独立行政法人農業・食品産業技術総合研究機構, 東北農業研究センター水田作研究領域, 主任研究員 (40455308)

Co-Investigator(Kenkyū-buntansha) ZENBAYASHI Kaoru  独立行政法人農業・食品産業技術総合研究機構, 東北農業研究センター水田作研究領域, 主任研究員 (80355320)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords病害抵抗性 / いもち病 / 圃場抵抗性遺伝子 / イネいもち病 / 抵抗性
Research Abstract

In this study, we have cloned the rice blast partial resistance gene Pi34 and comparedthe cytological reaction carrying Pi34 and complete resistance genes. RNAi and inoculation assay confirmed that OMG02 was Pi34. OMG02 was predicted as the gene of unknown function with two exons. Responses of rice sheath epidermal cells carrying Pi34 to rice blast infection were similar to those carrying a complete resistance gene. The other hand, the reactive oxygen species (ROS) accumulation in infected leaf blades epidermal cells of NILs carrying either Pi34 or a complete resistance gene indicated that manners of ROS accumulation were similar to each other in 24 hours post inoculation (hpi) but differed in 48hpi.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (17 results)

All 2013 2012 2011 2010 Other

All Journal Article (1 results) Presentation (15 results) Remarks (1 results)

  • [Journal Article] 真性抵抗性との比較によるいもち病圃場抵抗性遺伝子Pi34の細胞反応及び発現解析2011

    • Author(s)
      鬼頭英樹、善林薫、中島敏彦
    • Journal Title

      日本植物病理学会報

      Volume: 77 Pages: 220-220

    • Related Report
      2011 Annual Research Report
  • [Presentation] いもち病圃場抵抗性遺伝子Pi34の単離および感染初期応答における真性抵抗性との比較2013

    • Author(s)
      鬼頭英樹、善林薫
    • Organizer
      平成25年度日本植物病理学会大会
    • Place of Presentation
      岐阜市
    • Related Report
      2012 Final Research Report
  • [Presentation] いもち病圃場抵抗性遺伝子Pi34の単離および感染初期応答における真性抵抗性との比較2013

    • Author(s)
      鬼頭英樹
    • Organizer
      日本植物病理学会
    • Place of Presentation
      岐阜市
    • Related Report
      2012 Annual Research Report
  • [Presentation] いもち病圃場抵抗性遺伝子Pi34候補領域の解析2012

    • Author(s)
      鬼頭英樹、善林薫
    • Organizer
      日本植物病理学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-03-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cloning of rice blast resistance gene Pi34 and comparative analysis to explore a cue of durable resistance2012

    • Author(s)
      Hideki Kito, Kaoru Zenbayashi-Sawata
    • Organizer
      XV International Congress on Molecular Plant-Microbe Interactions abstracts
    • Place of Presentation
      京都市
    • Related Report
      2012 Final Research Report
  • [Presentation] いもち病圃場抵抗性遺伝子Pi34候補領域の解析2012

    • Author(s)
      鬼頭英樹、善林薫
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Final Research Report
  • [Presentation] Cloning of rice blast resistance gene Pi34 and comparative analysis to explore a cue of durable resistance2012

    • Author(s)
      鬼頭英樹
    • Organizer
      IS-MPMI 2012 XV International gongress
    • Place of Presentation
      京都市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 真性抵抗性との比較によるいもち病圃場抵抗性遺伝子Pi34の細胞反応および発現解析2011

    • Author(s)
      鬼頭英樹、善林薫、中島敏彦
    • Organizer
      平成23年度日本植物病理学会大会
    • Place of Presentation
      府中市
    • Related Report
      2012 Final Research Report
  • [Presentation] イネいもち病圃場抵抗性遺伝子Pi34座乗領域の構造および候補遺伝子の塩基配列解析2010

    • Author(s)
      善林薫
    • Organizer
      日本植物病理学会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2010-04-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 圃場抵抗性遺伝子Pi34が導入されたイネ準同質遺伝子系統のいもち病感染応答2010

    • Author(s)
      鬼頭英樹
    • Organizer
      日本植物病理学会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2010-04-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular and cellular characterization of a Koshihikari NIL introduced the partial resistance gene Pi342010

    • Author(s)
      Hideki Kito, Kaoru Zenbayashi-Sawata, Toshihiko Nakajima
    • Organizer
      The 5th International Rice Blast Conference
    • Place of Presentation
      アーカンソー州、アメ リカ合衆国
    • Related Report
      2012 Final Research Report
  • [Presentation] Cytological and genetic responses of near isogenic-lines carrying rice blast resistance genes2010

    • Author(s)
      Hideki Kito, Kaoru Zenbayashi-Sawata, Toshihiko Nakajima
    • Organizer
      APS annual meeting
    • Place of Presentation
      ノースカロライナ州, アメリカ合衆国
    • Related Report
      2012 Final Research Report
  • [Presentation] Fine Mapping of the Partial Resistance Gene to Blast2010

    • Author(s)
      Kaoru Zenbayashi-Sawata, Hideki Kito, Toshihiko Nakajima, Shinzo Koizumi
    • Organizer
      Pi34, and Anyalysis of its Gene-for-Gene Relationship in Rice, The 5th International Rice Blast Conference
    • Place of Presentation
      アーカンソー州,アメリカ合衆国
    • Related Report
      2012 Final Research Report
  • [Presentation] Fine Mapping of the Partial Resistance Gene to Blast, Pi34, and Anyalysis of its Gene-for-Gene Relationship in Rice2010

    • Author(s)
      善林薫
    • Organizer
      The 5th International Rice Blast Conference
    • Place of Presentation
      Little Rock, AR, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cytological and genetic responses of near isogenic-lines carrying rice blast resistance genes2010

    • Author(s)
      鬼頭英樹
    • Organizer
      2010 American Phytopathological Society meeting
    • Place of Presentation
      Charlotte, NC, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular and cellular characterization of a Koshihikari NIL introduced the partial resistance gene Pi342010

    • Author(s)
      鬼頭英樹
    • Organizer
      The 5th International Rice Blast Conference
    • Place of Presentation
      Little Rock, AR, USA
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.affrc.go.jp/ja/research/seika

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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