Characterization of a novel tripartite aromatic acids transporter, and its application for the production of industrially valuable metabolites

Research Project

Project/Area Number	22580077
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Applied microbiology
Research Institution	Nagaoka University of Technology
Principal Investigator	MASAI Eiji 長岡技術科学大学, 工学部, 教授 (20272867)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	フタル酸類 / テレフタル酸 / Comamonas属細菌 / 基質取り込み / 有用物質生産 / 生分解性ポリマー
Research Abstract	This study identified a novel type of aromatic acids transporter involved in terephthalate(TPA)uptake by Comamonas sp. strain E6. The TPA transporter is similar to the tripartite tricarboxylate transporter(TTT), and consists of the TpiA-TpiB membrane components and TPA-binding TphC. TPA uptake by E6 cells was completely inhibited by a protonophore, indicating that the TPA uptake system requires a proton motive force.

Report

(4 results)