Characterization of a novel tripartite aromatic acids transporter, and its application for the production of industrially valuable metabolites
Project/Area Number |
22580077
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied microbiology
|
Research Institution | Nagaoka University of Technology |
Principal Investigator |
MASAI Eiji 長岡技術科学大学, 工学部, 教授 (20272867)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | フタル酸類 / テレフタル酸 / Comamonas属細菌 / 基質取り込み / 有用物質生産 / 生分解性ポリマー |
Research Abstract |
This study identified a novel type of aromatic acids transporter involved in terephthalate(TPA)uptake by Comamonas sp. strain E6. The TPA transporter is similar to the tripartite tricarboxylate transporter(TTT), and consists of the TpiA-TpiB membrane components and TPA-binding TphC. TPA uptake by E6 cells was completely inhibited by a protonophore, indicating that the TPA uptake system requires a proton motive force.
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Report
(4 results)
Research Products
(5 results)