Characterization ofEcl1familygenesinvolvedinthechronological lifespan in fission yeast.

• Project/Area Number: 22580079
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Nagoya University
• Principal Investigator: AIBA Hirofumi 名古屋大学, 創薬科学研究科, 教授 (60211687)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 酵母 / 寿命 / 老化 / 分裂酵母 / 経時寿命 / Ecl1ファミリー / チェックポイント / マイクロアレイ / Ecl1 / 微生物 / ストレス応答

Research Abstract

Understanding of lifespan is one of the important theme in current biology. In this study I analyzed the function of Ecl1 family proteins that extend chronological lifespan of fission yeast when overproduced. I also carried out genetical screening to identify novel factors involved in the regulation of chronological lifespan in fission yeast. As a consequence, I have succeeded to reveal mechanisms of Ecl1 functioning and identified novel factors that are involved in the regulation of chronological lifespan in fission yeast. These results should contribute to better understanding of lifespan in higher eukaryote.

Research Products

• [Journal Article] 分裂酵母を用いた経時寿命制御因子の探索と機能解析 (2012)
  • Author(s): 饗場浩文、大塚北斗
  • Journal Title: 化学と生物 Volume: 50. No.5 Pages: 337-344
  • NAID: 10030819745
  • Peer Reviewed
• [Journal Article] Extension of chronological lifespan by ScEcl1 depends on mitochondria in Saccharomyces cerevisiae. (2012)
  • Author(s): Azuma, K., Ohtsuka, H., Murakami, H. and Aiba, H.
  • Journal Title: Biosci. Biotech. Biochem. Volume: 76(10) Pages: 1938-1942
  • NAID: 10031126484
  • Peer Reviewed
• [Journal Article] Another way to induce synchronous meiosis. (2012)
  • Author(s): Hiroshi Murakami, Hirofumi Aiba
  • Journal Title: Cell Cycle Volume: 11(10) Pages: 1874-1875
  • Peer Reviewed
• [Journal Article] Chronological lifespan extension by Ecl1 family proteins depends on Prr1 response regulator in fission yeast. (2012)
  • Author(s): Ohtsuka, H., Azuma, K., Kubota, S., Murakami, H.,Giga-Hama, Y.,Tohda,H. and Aiba, H.
  • Journal Title: Genes to Cells. Volume: 17 Pages: 39-52
  • Peer Reviewed
• [Journal Article] Extension of chronological lifespan by ScEcl1 depends on mitochondria in Saccharomyces cerevisiae (2012)
  • Author(s): Azuma, K.
  • Journal Title: Biosci. Biotech. Biochem. Volume: 76 Pages: 1938-1942
  • Peer Reviewed
• [Journal Article] Another way to induce synchronous meiosis. (2012)
  • Author(s): Murakami H, Aiba H.
  • Journal Title: Cell Cycle Volume: 15 Issue: 10 Pages: 1874-1874
  • DOI: 10.4161/cc.20511
  • Peer Reviewed
• [Journal Article] 分裂酵母を用いた経時寿命制御因子の探索と機能解析 (2012)
  • Author(s): 饗場浩文
  • Journal Title: 化学と生物 Volume: 50 Pages: 337-344
  • NAID: 10030819745
  • Peer Reviewed
• [Journal Article] Chronological lifespan extension by Ecl1 family proteins depends on Prr1 response regulator in fission yeast (2012)
  • Author(s): Ohtsuka, H.
  • Journal Title: Genes to Cells Volume: 17 Issue: 1 Pages: 39-52
  • DOI: 10.1111/j.1365-2443.2011.01571.x
  • Peer Reviewed
• [Journal Article] Ecl1, a regulator ofthechronologicallifespan of Schizosaccharomyces pombe , is induced upon nitrogen starvation. (2011)
  • Author(s): Miwa, Y., Ohtsuka, H., Naito, C., Murakami, H. and Aiba, H.
  • Journal Title: Biosci. Biotech.Biochem. Volume: 75 Pages: 279-283
  • Peer Reviewed
• [Journal Article] hsf1^+ extends chronological lifespan through Ecl1 family genes in fission yeast. (2011)
  • Author(s): Ohtsuka, H., Azuma, K., Murakami, H., and Aiba, H.
  • Journal Title: Mol. Genet. Genomics Volume: 285 Pages: 67-77
  • Peer Reviewed
• [Journal Article] Ecl1, a regulator of the chronological lifespan of Schizosaccharomyces pombe, is induced upon nitrogen starvation. (2011)
  • Author(s): Miwa Y., Ohtsuka H., Naito C., Murakami H., Aiba H.
  • Journal Title: Biosci.Biotech.Biochem. Volume: 75 Pages: 279-283
  • NAID: 10027897689
  • Peer Reviewed
• [Journal Article] hsf1^+ extends chronological lifespan through Ecl1 family genes in fission yeast. (2011)
  • Author(s): Ohtsuka, H., Azuma, K., Murakami, H., Aiba, H.
  • Journal Title: Mol.Genet.Genomics Volume: 285 Pages: 67-77
  • Peer Reviewed
• [Journal Article] Regulation of yea st forkhead transcription factors and FoxM1 bycyclin-dependent andPolo-like kinases. (2010)
  • Author(s): Hiroshi Murakami, Hirofumi Aiba , Makoto Nakanishi and Yuko Murakami-Tonami
  • Journal Title: Cell Cycle Volume: 9 (16) Pages: 3233-3242
  • Peer Reviewed
• [Journal Article] Pma1, a P-type proton ATPase, i s a determinant of chronological lifespan in fission yeast. (2010)
  • Author(s): Ito, H.,Oshiro,T.,Fujita,Y.,Kubota, S., Ohtsuka,H., H.,Naito,C.,Murakami, and Aiba, H.
  • Journal Title: J. Biol. Chem. Volume: 285 Pages: 34616-34620
  • Peer Reviewed
• [Journal Article] Regulation of yeast forkhead transcription factors and FoxM1 by cyclin-dependent and Polo-like kinases. (2010)
  • Author(s): Murakami, H., Aiba H., Nakanishi, M., Murakami-Tonami, Y.
  • Journal Title: Cell Cycle Volume: 9 Pages: 3233-3242
  • Peer Reviewed
• [Journal Article] Pma1, a P-type proton ATPase, is a determinant of chronological lifespan in fission yeast. (2010)
  • Author(s): Ito, H., Oshiro, T., Fujita, Y., Kubota, S., Naito, C., Ohtsuka, H., Murakami, H., Aiba, H.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 34616-34620
  • Peer Reviewed
• [Presentation] Pma1, a P-type proton ATPase, is a determinant of chronological lifespan in fission yeast (2013)
  • Author(s): Hirokazu Ito, Tomoko Oshiro, Hokuto Ohtsuka, and Hirofumi Aiba.
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: Nagoya
• [Presentation] Identification of novel genes, ecl1^+, ecl2^+, and ecl3^+, which extend chronological lifespan in fission yeast (2013)
  • Author(s): Hokuto Ohtsuka, Kenko Azuma, and Hirofumi Aiba.
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: Nagoya
• [Presentation] Functional characterization of lifespan elongation factor Ecl1 in Saccharomyces cerevisiae (2013)
  • Author(s): Kenko Azuma, Hokuto Ohtsuka, Yurie Koga, Chikako Naito, Hiroshi Murakami, and Hirofumi Aiba.
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: Nagoya
• [Presentation] Php2 mutant extends the chronological lifespan of fission yeast (2013)
  • Author(s): Kazuaki Takuma, Hokuto Ohtsuka, Kenko Azuma, Hiroshi Murakami and Hirofumi Aiba.
  • Organizer: Php2 mutant extends the chronological lifespan of fission yeast.
  • Place of Presentation: Nagoya
• [Presentation] A novel gene SPBC16A3.08c which extends chronological lifespan in fission yeast functions downstream of TOR pathway (2013)
  • Author(s): Shingo Ogawa, Hokuto Ohtuka, Erika Sakai, Hideaki Kawamura, Hiroshi Murakami, and Hirofumi Aiba.
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: Nagoya
• [Presentation] Identification of novel genes, ecl1+, ecl2+, and ecl3+, which extend chronological lifespan in fission yeast (2013)
  • Author(s): Hokuto Ohtsuka
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: Nagoya
• [Presentation] Php2 mutant extends the chronological lifespan of fission yeast (2013)
  • Author(s): Kazuaki Takuma
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: Nagoya
• [Presentation] 健康長寿社会をめざす酵母を用いた寿命研究の試み (2012)
  • Author(s): 饗場浩文
  • Organizer: 日本薬学会東海支部特別講演会
  • Place of Presentation: 金城学院大学
  • Year and Date: 2012-12-17
• [Presentation] 酵母のCpc2欠損における胞子形成欠陥を相補する (2012)
  • Author(s): 東剣虹、大塚北斗、古賀由梨枝、内藤知佳子、村上浩士、饗場浩文
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] 分裂酵母Php2の変異によって経時寿命が長くなる (2012)
  • Author(s): 琢磨和晃、大塚北斗、東剣虹、村上浩士、饗場浩文
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] 分裂酵母の新規経時寿命延長遺伝子SPBC16A3.08cはTOR経路の下流で働く (2012)
  • Author(s): 小川真悟、大塚北斗、酒井枝里香、川村英彰、村上浩士、饗場浩文
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] 分裂酵母における経時寿命延長因子Ecl1 の解析 (2012)
  • Author(s): 島崎嵩史、大塚北斗、内藤知佳子、村上浩士、饗場浩文
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-10-27
• [Presentation] 分裂酵母におけるRACK1ホモログCpc2についての研究 (2012)
  • Author(s): 東剣虹、大塚北斗、古賀由梨枝、内藤知佳子、村上浩士、饗場浩文
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-10-27
• [Presentation] 分裂酵母php2+が経時寿命に与える影響に関する研究 (2012)
  • Author(s): 琢磨和晃、大塚北斗、東剣虹、村上浩士、饗場浩文
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-10-27
• [Presentation] 分裂酵母の新規経時寿命延長因子に関する研究 (2012)
  • Author(s): 小川真悟、大塚北斗、酒井枝里香、川村英彰、村上浩士、饗場浩文
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋
  • Year and Date: 2012-10-27
• [Presentation] 分裂酵母を用いた経時寿命延長因子の解析 (2012)
  • Author(s): 饗場浩文
  • Organizer: 日本農芸化学会2012年度大会シンポジウム「健康と長寿に貢献する酵母研究」
  • Place of Presentation: 京都
  • Year and Date: 2012-03-25
• [Presentation] 分裂酵母を用いた経時寿命延長因子の解析 (2012)
  • Author(s): 饗場浩文
  • Organizer: 日本農芸化学会2012年度大会シンポジウム
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2012-03-25
• [Presentation] php2変異株は分裂酵母の経時寿命を延長させる (2012)
  • Author(s): 大塚北斗、琢磨和晃、東剣虹、村上浩士、饗場浩文
  • Organizer: 酵母遺伝学フォーラム第45回研究報告会
  • Place of Presentation: 京都
• [Presentation] 分裂酵母におけるEcl1ファミリー遺伝子による経時寿命延長はPrrlに依存する (2011)
  • Author(s): 大塚北斗、東剣虹、窪夛佐知子、村上浩士、浜祐子、東田英毅、饗場浩文
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] 分裂酵母の経時寿命延長因子Ecl1ファミリーが有性生殖に与える影響 (2011)
  • Author(s): 内藤知佳子、大塚北斗、村上浩士、饗場浩文
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] ScEcl1 extends chronological lifespan depending on mitochondria (2011)
  • Author(s): K.Azuma, H.Ohtsuka, H.Murakami, H.Aiba
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] 酵母に学ぶ寿命研究が拓く未来 名古屋大学 (2011)
  • Author(s): 饗場浩文
  • Organizer: 創薬を目指した融合領域サマーセミナー2011~最先端メディシナルサイエンスの未来~
  • Place of Presentation: 名古屋大学
  • Year and Date: 2011-09-03
• [Presentation] 酵母に学ぶ寿命研究が拓く未来 (2011)
  • Author(s): 饗場浩文
  • Organizer: 創薬を目指した融合領域サマーセミナー2011～最先端メディシナルサイエンスの未来～
  • Place of Presentation: 名古屋大学
  • Year and Date: 2011-09-03
• [Presentation] 分裂酵母の経時寿命延長変異株の解析 (2011)
  • Author(s): 饗場浩文、伊藤宏和、大城智子、藤田康之、窪夛佐知子、内藤知佳子、大塚北斗、村上浩士
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-05
• [Presentation] Pma1, a P-type proton ATPase, is a determinant of chronological lifespan in fission yeast (2011)
  • Author(s): Hirokazu Ito, Tomoko Oshiro, Yasuyuki Fujita, Sachiko Kubota, Chikako Naito, Hokuto Ohtsuka, Hiroshi Murakami, and Hirofumi Aiba.
  • Organizer: The 6 th international fission yeast meeting
  • Place of Presentation: Boston, USA
• [Presentation] Chronological lifespan extension by Ecl1 family genes depends on prr1+ in fission yeast (2011)
  • Author(s): Hokuto Ohtsuka, Kenko Azuma, Sachiko Kubota, Hiroshi Murakami, Yuko -Giga-Hama, Hideki Tohda, and Hirofumi Aiba.
  • Organizer: The 6 th international fission yeast meeting
  • Place of Presentation: Boston, USA
• [Presentation] Regulation of wee1^+ expression during meiosis in fission yeast (2011)
  • Author(s): Yuko Murakami-Tonami, Hokuto Ohtsuka, Makoto Nakanishi, Hirofumi Aiba and Hiroshi Murakami.
  • Organizer: The 6 th international fission yeast meeting
  • Place of Presentation: Boston, USA
• [Presentation] Identification and characterization of determinants of chronological lifespaninfissionyeast (2011)
  • Author(s): Hirofumi Aiba.
  • Organizer: 5thThe International Workshop on Cell Regulations in Division and Arrest
  • Place of Presentation: Okinawa, OIST Seaside House
• [Presentation] Identification and characterization of determinants of chronological lifespan in fission yeast (2011)
  • Author(s): Hirofumi Aiba
  • Organizer: The 5^<th> International Workshop on Cell Regulations in Division and Arrest
  • Place of Presentation: Okinawa, OIST Seaside House(招待講演)
• [Presentation] Regulation of weel^+ expression during meiosis in fission yeast (2011)
  • Author(s): Yuko Murakami-Tonami, Hokuto Ohtsuka, Makoto Nakanishi, Hirofumi Aiba, Hiroshi Murakami
  • Organizer: The 6 th international fission yeast meeting
  • Place of Presentation: Boston, USA
• [Presentation] Chronological lifespan extension by Ecll family genes depends on prrl^+ in fission yeast (2011)
  • Author(s): Hokuto Ohtsuka, Kenko Azuma, Sachiko Kubota, Hiroshi Murakami, Yuko-Giga-Hama, Hideki Tohda, Hirofumi Aiba
  • Organizer: The 6 th international fission yeast meeting
  • Place of Presentation: Boston, USA
• [Presentation] Pmal, a P-type proton ATPase, is a determinant of chronological lifespan in fission yeast (2011)
  • Author(s): Hirokazu Ito, Tomoko Oshiro, Yasuyuki Fujita, Sachiko Kubota, Chikako Naito, Hokuto Ohtsuka, Hiroshi Murakami, Hirofumi Aiba
  • Organizer: The 6 th international fission yeast meeting
  • Place of Presentation: Boston, USA
• [Presentation] 分裂酵母Hsf1はEcl遺伝子を介して経時寿命を延長させる (2010)
  • Author(s): 大塚北斗、東剣虹、村上浩士、饗場浩文
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] S.cerevisiaeにおけるEcl1ファミリー遺伝子の特定と解析 (2010)
  • Author(s): 東剣虹、大塚北斗、内藤知佳子、村上浩士、饗場浩文
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] 分裂酵母の経時寿命延長因子Ecl1ファミリーの機能発現に重要なアミノ酸の解析 (2010)
  • Author(s): 内藤知佳子、大塚北斗、村上浩士、饗場浩文
  • Organizer: 日本農芸化学会中部支部第159回例会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2010-10-30
• [Presentation] 分裂酵母の経時寿命延長因子であるEcl1の発現機構の解析 (2010)
  • Author(s): 三輪由紀子、大塚北斗、内藤知佳子、村上浩士、饗場浩文
  • Organizer: 日本農芸化学会中部支部第159回例会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2010-10-30
• [Presentation] 大腸菌の全二成分制御系遺伝子プロモーターの強度測定 (2010)
  • Author(s): 山下和哉、饗場浩文、石浜明、山本兼由
  • Organizer: 第9回微生物研究会
  • Place of Presentation: 法政大学
  • Year and Date: 2010-06-26
• [Presentation] 分裂酵母Hsf1はEcl1,2,3を介して経時寿命を延長させる (2010)
  • Author(s): 大塚北斗、東剣虹、村上浩士、饗場浩文
  • Organizer: 酵母遺伝学フォーラム第43回研究報告会
  • Place of Presentation: ならまちセンター(奈良)
• [Presentation] S.cerevisiaeにおける分裂酵母Ecl1ファミリーの解析 (2010)
  • Author(s): 東剣虹、大塚北斗、村上浩士、饗場浩文
  • Organizer: 酵母遺伝学フォーラム第43回研究報告会
  • Place of Presentation: ならまちセンター(奈良)
• [Presentation] Identification of novel genes, ecl1^+, ecl2^+, and ecl3^+, which extend chronological lifespan in fission yeast. (2010)
  • Author(s): 大塚北斗、東剣虹、饗場浩文
  • Organizer: 名古屋大学グローバルCOE第4回リトリート
  • Place of Presentation: 豊橋
• [Presentation] Casein kinase II is involved in the spindle assembly checkpoint by regulating Mad2 in fission yeast. (2010)
  • Author(s): Midori Shimada, Ayumu Yamamoto, Yuko Murakami-Tonami, Makoto Nakanishi, Hirofumi Aiba, Hiroshi Murakami.
  • Organizer: The 2010 Yeast Genetics and Molecular Biology Meeting
  • Place of Presentation: University of British Columbia, Vancouver, Canada
• [Presentation] 分裂酵母の新規経時寿命延長遺伝子SPBC16A3.08cはTOR経路の下流で働く
  • Author(s): 小川真悟
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 分裂酵母Php2の変異によって経時寿命が長くなる
  • Author(s): 琢磨和晃
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] ロイシンは分裂酵母のCpc2欠損における胞子形成欠陥を相補する
  • Author(s): 東剣虹
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 分裂酵母の新規経時寿命延長因子に関する研究
  • Author(s): 小川真悟
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋
• [Presentation] 分裂酵母php2+が経時寿命に与える影響に関する研究
  • Author(s): 琢磨和晃
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋
• [Presentation] 分裂酵母におけるRACK1ホモログCpc2についての研究
  • Author(s): 東剣虹
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋
• [Presentation] 分裂酵母における経時寿命延長因子Ecl1の解析
  • Author(s): 島崎嵩史
  • Organizer: 日本農芸化学会中部支部第165回例会
  • Place of Presentation: 名古屋