Pathway engineering of ethanologenic bacteria based on HiCEP (High Coverage Expression Profiling)analysis

• Project/Area Number: 22580084
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tottori University
• Principal Investigator: YANASE Hideshi 鳥取大学, 大学院・工学研究科, 教授 (20158033)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: バイオマスエネルギー / 微生物遺伝学 / 代謝工学 / bioethanol / biomass / genomics / DNA microarray / transcriptome / Zymomonas mobilis / Zymobacter palmae / biofuel / ethanol / microarray / transcription / Zymobacter palame

Research Abstract

To improve the tolerance of Zymobacter palmae to various stress during cellulosic bioethanol fermentation, we have been engineering genetically a robust Zb. palmae based on the gene expression profiling responding to fermentation stress by HiCEP and DNA microarray analyses. We found the first mechanism acquiring the acid tolerance depended on the amino acid biosynthetic pathway.

Research Products

• [Journal Article] Direct ethanol production from cellulosic materials by Zymobacter palmae carrying Cellulomonas and Ruminococcus -glucosidase (2013)
  • Author(s): Kojima, M., Okamoto, K., and Yanase,H.
  • Journal Title: Appl. Microbiol. Biotechno Volume: 97 Issue: 11 Pages: 5137-5147
  • DOI: 10.1007/s00253-013-4874-1
  • Year and Date: 2013-04-21
  • Peer Reviewed
• [Journal Article] Expression and surface display of Cellulomonas endoglucanase in the ethanologenic bacterium Zymobacter palmae (2012)
  • Author(s): Kojima, M., Akahoshi, T., Okamoto, K.,and Yanase, H.
  • Journal Title: Appl. Microbiol. Biotechno Volume: 96
  • Peer Reviewed
• [Journal Article] Ethanol production from wood hydrolysate using genetically engineered Zymomonas mobilis (2012)
  • Author(s): Yanase, H., Miyawaki, H., Sakurai, M.,Kawakami, A., Matsumoto, M., Haga, K.,Kojima, M., and Okamoto, K.
  • Journal Title: Appl. Microbiol. Biotechnol. Volume: 94
  • Peer Reviewed
• [Journal Article] Ethanol production from wood hydrolysate using genetically engineered Zymomonas mobilis (2012)
  • Author(s): Hideshi Yanase
  • Journal Title: Appl. Microbiol. Biotechnol. Volume: 94 Pages: 1667-1678
  • Peer Reviewed
• [Journal Article] Expression and surface display of Cellulomonas endoglucanase in the ethanologenic bacterium Zymobacter palmae (2012)
  • Author(s): Motoki Kojima
  • Journal Title: Appl. Microbiol. Biotechnol Volume: 96 Issue: 4 Pages: 1093-1104
  • DOI: 10.1007/s00253-012-4424-2
  • Peer Reviewed
• [Journal Article] Efficient xylose fermentation by the brown rot fungus Neolentinus lepideus (2012)
  • Author(s): Okamoto, K., Kanawaku, R., Masumoto, M., Yanase, H.
  • Journal Title: Enzyme Microb.Technol. Volume: 50 Pages: 96-100
  • Peer Reviewed
• [Journal Article] Characterization of the protein gp1 from bacteriophage In 93 (2011)
  • Author(s): Matsushita I., and Yanase H.
  • Journal Title: Biological Lett Volume: 48(1) Pages: 47-56
  • Peer Reviewed
• [Journal Article] Characterization of two acidic ss-glucosidases and ethanol fermentation in the brown rot fungus Fomitops is palustris (2011)
  • Author(s): Okamoto, K., Sugita, Y., Nishikori, N., Nitta, Y., Yanase, H.
  • Journal Title: Enzyme and Microbial Technology Volume: 48 Pages: 96-100
  • Peer Reviewed
• [Journal Article] Direct ethanol production from starch, wheat bran and rice straw by the white rot fungus Trametes hirsuta (2011)
  • Author(s): Okamoto, K., Nitta, Y., Maekawa, N., Yanase, H.
  • Journal Title: Enzyme Microb.Technol. Volume: 48 Pages: 273-277
  • Peer Reviewed
• [Journal Article] Characterization of the protein gp1 from bacteriophage φIN93 (2011)
  • Author(s): Matsushita I., Yanase H.
  • Journal Title: Biological Lett. Volume: 48(1) Pages: 47-56
  • Peer Reviewed
• [Journal Article] Production of ethanol by the white-rot basidiomycetes Peniophora cinerea and Trametes sunveolens (2010)
  • Author(s): Kenji Okamoto, Koji Imashiro, Yuya Akizawa, Asako Onimura, Motoki Yoneda, Yasuyuki Nitta, Nitaro Maekawa, Hideshi Yanase
  • Journal Title: Biotechnol.Lett. Volume: 32(7) Pages: 909-913
  • Peer Reviewed
• [Journal Article] Direct ethanol production from starch, wheat bran and rice straw by the white rot fungus Trametes hirsuta (2010)
  • Author(s): Kenji Okamoto, Yasuyuki Nitta, Nitaro Maekawa, Hideshi Yanase
  • Journal Title: Enzyme Microb.Technol. Volume: doi:10.1016(電子版)
  • Peer Reviewed
• [Journal Article] Characterization of two acidic β-glucosidases and ethanol fermentation in the brown rot fungus Fomitopsis palustris (2010)
  • Author(s): Kenji Okamoto, Yuko Sugita, Natsumi Nishikori, Yasuyuki Nitta, Hideshi Yanase
  • Journal Title: Enzyme Microb.Technol. Volume: doi:10.1016(電子版)
  • Peer Reviewed
• [Journal Article] マイクロ波ソルボリシスと高速発酵細菌を用いる高効率バイオエタノール生産システム (2010)
  • Author(s): 渡辺隆司、簗瀬英司
  • Journal Title: クリーンエネルギー Volume: 19(12) Pages: 1-6
  • Peer Reviewed
• [Presentation] C5・C6糖並行発酵性Zymobacter palmaeのキシロース発酵性強化 (2012)
  • Author(s): 滝上、近藤、奥田、岡本、原田、簗瀬
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-10-26
• [Presentation] ラッカーゼ処理によるリグニン過分解物発酵阻害の低減効果 (2012)
  • Author(s): 伊原、高橋、杉岡、岡本、原田、簗瀬
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-10-26
• [Presentation] Cellvibrio japonicus由来セルラーゼ遺伝子のクローニングと発酵細菌内での分泌発現 (2012)
  • Author(s): 小島、幡部、玉井、岡本、原田、簗瀬
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-10-26
• [Presentation] キシロース並行発酵細菌によるマイクロ波照射前処理木材のSSCF(同時糖化並行発酵) (2012)
  • Author(s): 簗瀬英司、小島基、高橋貴之、黒崎陽介、大代正和、桂陽子、瀬郷久幸、松下響、保谷典子、渡辺隆司
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-24
• [Presentation] バイオマスエネルギー技術の現状と将来 (2012)
  • Author(s): 簗瀬英司
  • Organizer: 日本機械学会中国四国支部第114講習会
  • Place of Presentation: 鳥取
  • Year and Date: 2012-01-20
• [Presentation] バイオマスエネルギー技術の現状と将来 (2012)
  • Author(s): 簗瀬英司
  • Organizer: 日本機械学会中国四国支部第114講習会
  • Place of Presentation: 鳥取(招待講演)
  • Year and Date: 2012-01-20
• [Presentation] Ｃ５・Ｃ６糖並行発酵性Zymobacter palmaeのキシロース発酵性強化 (2012)
  • Author(s): 簗瀬英司
  • Organizer: 日本生物工学会
  • Place of Presentation: 東京農工大
• [Presentation] ラッカーゼ処理によるリグニン過分解物発酵阻害の低減効果 (2012)
  • Author(s): 簗瀬英司
  • Organizer: 日本生物工学会
  • Place of Presentation: 東京農工大
• [Presentation] Cellvibrio japonicus由来セルラーゼ遺伝子のクローニングと発酵細菌内での分泌発現 (2012)
  • Author(s): 簗瀬英司
  • Organizer: 日本生物工学会
  • Place of Presentation: 東京農工大
• [Presentation] Ethanol production from pretreated eucalyptus wood chips by simultaneous saccharification and fermentation RAFT (2011)
  • Author(s): Matsushita H, Yanase H, Daidai M, Sego H,Yasutani N, Habu K, Kikuta H, Yasue Y,Watanabe T
  • Organizer: Recent Advances in Fermentation Technology
  • Place of Presentation: 米国
  • Year and Date: 2011-11-08
• [Presentation] Ethanol production from pretreated eucalyptus wood chips by simultaneous saccharification and fermentation (2011)
  • Author(s): Matsushita H, Yanase H, Daidai M, Sego H, Yasutani N, Habu K, Kikuta H,Yasue Y, Watanabe T
  • Organizer: RAFT(Recent Advances in FermentationTechnology)
  • Place of Presentation: 米国
  • Year and Date: 2011-11-08
• [Presentation] マイクロ波前処理および高速発酵細菌を用いた木質バイオマスからのバイオエタノール生産 (2011)
  • Author(s): 大代正和、三谷友彦、篠原真毅、吉岡康一、黒崎陽介、金子正和、渡辺隆司、瀬郷久幸、桂陽子、泰江康輝、保谷典子、菊田弘和、金川章孝、高橋和志、小島基、簗瀬英司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-27
• [Presentation] セルラーゼとリグニンの相互作用解析 (2011)
  • Author(s): 高田理江、吉岡康一、小島基、簗瀬英司、渡辺隆司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-27
• [Presentation] C5・C6糖並行発酵性Zymobacter palmaeのキシロース発酵に及ぼすリグノセルロース過分解物の影響 (2011)
  • Author(s): 高橋貴之、小島基、岡本賢治、簗瀬英司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-27
• [Presentation] 高糖耐性Zymomonas mobilisへのキシロース並行発酵性の付与 (2011)
  • Author(s): 小島基、岡本賢治、簗瀬英司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-27
• [Presentation] 高糖耐性Zymomonas mobilisへのC5・C6糖並行発酵性付与 (2011)
  • Author(s): 近藤雄亮、羽賀俊雄、岡本賢治、簗瀬英司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-27
• [Presentation] Metabolic engineering of ethanologenic bacteria capable of producing cellulosic bioethanol (2011)
  • Author(s): Yanase,H.
  • Organizer: International Union of Microbiological Soceities 2011 Congress
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-09
• [Presentation] Metabolic engineering of ethanologenic bacteria capable of producing cellulosic bioethanol (2011)
  • Author(s): Yanase H
  • Organizer: International Union of Microbiological Soceities 2011 Congress
  • Place of Presentation: 札幌(招待講演)
  • Year and Date: 2011-09-09
• [Presentation] 新規キシロース発酵担子菌によるエタノール生産 (2011)
  • Author(s): 内井敦史、岡本賢治、簗瀬英司
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-28
• [Presentation] 褐色腐朽菌によるキシロースからの効率的エタノール生産 (2011)
  • Author(s): 金涌龍一、岡本賢治、簗瀬英司
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-28
• [Presentation] 担子菌Trametes hirsutaによるバイオマスからの直接的エタノール生産 (2011)
  • Author(s): 岡本賢治、新田泰之、簗瀬英司
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-28
• [Presentation] C5・C6糖並行発酵性Zymobacter palmaeのリグノセルロース過分解物による発酵阻害挙動 (2011)
  • Author(s): 高橋貴之、小島基、岡本賢治、簗瀬英司
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-28
• [Presentation] 多機能EGとBGLの共発現化によるZymobacter palmaeのセルロース糖化醗酵 (2011)
  • Author(s): 小島基、岡本賢治、簗瀬英司
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-28
• [Presentation] 高糖耐性Zymomonas mobilisへのC5・C6糖並行発酵性付与 (2011)
  • Author(s): 近藤雄亮、羽賀俊雄、岡本賢治、簗瀬英司
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-28
• [Presentation] C5・C6糖並行発酵性Zymobacter palmaeの糖代謝遺伝子発現挙動解析 (2010)
  • Author(s): 東裏典枝、岡崎悟志、岡本賢治、簗瀬英司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-10-27
• [Presentation] 多機能エンドグルカナーゼ遺伝子のクローニングとエタノール発酵細菌内での分泌発現 (2010)
  • Author(s): 小島基、松浦静香、岡本賢治、簗瀬英司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-10-27
• [Presentation] 高糖耐性Zymomonas mobilis野生株へのセルロース糖化は構成賦与 (2010)
  • Author(s): 奥田洋、小島基、岡本賢治、簗瀬英司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-10-27
• [Presentation] Peniophora cinereaによるキシリトール生産 (2010)
  • Author(s): 藤村拓生、岡本賢治、簗瀬英司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-10-26
• [Presentation] Peniophora属担子菌によるエタノール生産 (2010)
  • Author(s): 米田元輝、秋沢佑弥、岡本賢治、簗瀬英司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-10-26
• [Presentation] Fomitopsis palustris由来β-glucosidaseとエタノール発酵 (2010)
  • Author(s): 岡本賢治、杉田佑子、錦織なつみ、新田泰之、簗瀬英司
  • Organizer: 日本生物工学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-10-26
• [Patent(Industrial Property Rights)] マンノース発酵性ザイモバクター属形質転換微生物 (2011)
  • Inventor(s): 簗瀬英司、岡本賢治
  • Industrial Property Rights Holder: 国立大学法人鳥取大学
  • Acquisition Date: 2011-02-25
• [Patent(Industrial Property Rights)] 染色体組み込みマンノース発酵性ザイモモナス属細菌 (2010)
  • Inventor(s): 簗瀬英司、岡本賢治
  • Industrial Property Rights Holder: 国立大学法人鳥取大学
  • Acquisition Date: 2010-06-18