Characterization of indolocarbazole biosynthetic enzymes; for the application to

• Project/Area Number: 22580089
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: The University of Tokyo (2012); Toyama Prefectural University (2010-2011)
• Principal Investigator: ONAKA Hiroyasu 東京大学, 大学院・農学生命科学研究科, 特任教授 (80315829)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 遺伝学 / 応用微生物 / 抗生物質 / 発酵 / バイオテクノロジー / 生合成 / 放線菌 / 遺伝子組換え / インドロカルバゾール / 抗がん物質 / 抗ガン物質 / 酸化酵素

Research Abstract

Indolocarbazole (ICZ) skeleton is biosynthesized from tryptophane as a precursor byfour biosynthetic enzymes. In this study, the biosynthetic enzymes involved instaurosporine, one of ICZ are characterized. StaD is a coupling enzyme of twomolecules of indolepyruvic acid. We detected the intermediate of the enzymaticreaction and determine the chemical structure. We then analyzed how StaC catalyzesthe formation of K252c by using of mutated StaC.

Research Products

• [Journal Article] Coupling Reaction of Indolepyruvic Acid by StaD and Its Product: Implications for Biosynthesis of Indolocarbazole and Violacein (2012)
  • Author(s): *Nagano, S.(3) Hino, T., (他5名)
  • Journal Title: ChemBioChem Volume: 13 Issue: 17 Pages: 2495-2500
  • DOI: 10.1002/cbic.201200535
  • Peer Reviewed
• [Journal Article] Characterization and Functional Modification of StaC and RebC, Which Are Involved in the Pyrrole Oxidation of Indolocarbazole Biosynthesis (2011)
  • Author(s): *Nagano, S.(6)(他4名)
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 11 Pages: 2184-2193
  • DOI: 10.1271/bbb.110474
  • NAID: 10030396835
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Presentation] 放線菌由来インドロカルバゾール生合成酵素群の反応機構 (2011)
  • Author(s): 尾仲宏康
  • Organizer: 酵素工学研究会講演会
  • Place of Presentation: 酵素工学研究会
  • Year and Date: 2011-09-29
• [Presentation] Characterization of indolocarbazole biosynthetic enzymes in Streptomyces sp.TP-A0274 (2011)
  • Author(s): 尾仲宏康
  • Organizer: 16th Japanese-German Workshop on Enzyme Technology
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2011-09-14
• [Presentation] Tryptophan determination by using tryptophan oxidases involved in bis-indole antibiotics biosynthesis (2011)
  • Author(s): Masafumi Kameya
  • Organizer: 16th Japanese-German Workshop on Enzyme Technology
  • Place of Presentation: 富山国際会議場
  • Year and Date: 2011-09-14
• [Presentation] 放線菌由来ヘテロ環含有抗生物質の生合成に関する分子生物学的研究 (2010)
  • Author(s): 尾仲 宏康
  • Organizer: 日本農芸化学会中部支部会例会
  • Place of Presentation: 静岡市
  • Year and Date: 2010-05-30
• [Presentation] 放線菌由来ヘテロ環含有抗生物質の生合成に関する分子生物学的研究 (2010)
  • Author(s): 尾仲宏康
  • Organizer: 日本農芸化学会中部支部会例会(20年度日本農芸化学奨励賞受賞講演)
  • Place of Presentation: 静岡市
  • Year and Date: 2010-05-30
• [Remarks] 微生物潜在機能探索寄付講座ホームページ
  • URL: http://microbial-potential.bt.a.u-tokyo.ac.jp/