Elucidation of the mechanisms for fruiting body formation founded on light signaltransduction

• Project/Area Number: 22580097
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: MIYAZAKI Yasumasa 独立行政法人森林総合研究所, きのこ・微生物研究領域, 主任研究員 (40343800)
• Co-Investigator(Kenkyū-buntansha): KANEKO Shinya 東京工業大学, 大学院生命理工学研究科, 助教 (10399694)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: きのこ / 光受容体 / 形態変化 / シイタケ / 子実体形成 / 光応答 / バイオインフォマティクス / 遺伝子発現 / 次世代型DNAシーケンサー / 遺伝子 / ゲノム / 応用微生物 / シグナル伝達 / 生体分子 / 農林水産物 / 発生・分化 / 子実体

Research Abstract

Fruiting body formation of mushroom requires light. The Le.cry gene in L. edodes encoded a cryptochrome photo receptor first isolated from basidiomycetes. The expression product, Le.CRY, was suggested to be involved in fruiting body formation via perception of blue light. As a result of proteome analysis, there existed metabolisms and signal transduction pathways involved in fruiting body formation, which were mediated by protein phosphorylation and glycosylation. Photo-responsive genes were comprehensively identified by transcriptome analysis.

Research Products

• [Journal Article] Gene expression profiling of the baisidiomycetous fungus Lentinula edodes after light stimulation. (2013)
  • Author(s): Sano H, Sakamoto Y, Abe M, Kaneko S, Nakamura M, Miyazaki Y.
  • Journal Title: Fungal Genetics Reports Volume: 60 Pages: 228-228
• [Journal Article] Ectomycorrhizal formation on regenerated somatic pine plants after inoculation with Tricholoma matsutake. (2012)
  • Author(s): Yamanaka T, Maruyama T, Yamada A, Miyazaki Y, Kikuchi T
  • Journal Title: Mushroom Sci.ence and Biotechnology Volume: 20 Pages: 93-97
  • NAID: 10031125206
  • Peer Reviewed
• [Journal Article] Light-stimulative effects on the cultivation of edible mushrooms by using blue LED (2011)
  • Author(s): Miyazaki Y, Masuno K, Abe M, Nishizawa H, Matsumoto T, Kunitomo S, Sakata H, Nakamura K, Koyama T, Ito M, Kazama H, Suzuki D, Obatake Y, Sano H, Nakamura M, Miyazaki K, Sakamoto Y, Kaneko S, Kamada
  • Journal Title: Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products Volume: 7 Pages: 58-67
• [Journal Article] A DASH-type cryptochrome gene from Lentinula edodes (2011)
  • Author(s): Sano H, Kaneko S, Nakamura M, Miyazaki Y
  • Journal Title: Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products Volume: 7 Pages: 72-81
• [Journal Article] Differentially expressed genes under simulated microgravity in fruiting bodies of the fungus Pleurotus ostreatus (2010)
  • Author(s): Yasumasa Miyazaki, Masahide Sunagawa, Akira Higashibata, Noriaki Ishioka, Katsuhikot Babasaki, Takashi Yamazaki.
  • Journal Title: FEMS Microbiology Letters Volume: 307 Pages: 72-79
  • Peer Reviewed
• [Journal Article] Differentially expressed genes under simulated microgravity in fruiting bodies of the fungus Pleurotus ostreatus. (2010)
  • Author(s): Miyazaki Y, Sunagawa M, Higashibata A, Ishioka N, Babasaki K, Yamazaki T.
  • Journal Title: FEMS Microbiology Letters Volume: 307 Pages: 72-79
  • Peer Reviewed
• [Presentation] シイタケ品種の同定のためのIGS1領域の塩基配列情報の拡充について (2012)
  • Author(s): 宮崎和弘，宮崎安将
  • Organizer: 日本きのこ学会第16回大会講演要旨集
• [Presentation] シイタケゲノムの解読と公開 (2012)
  • Author(s): 宮崎安将、金子真也、高野麻理子、中村雅哉、村田仁、馬場崎勝彦
  • Organizer: 東京大学グローバルCOEプログラム ゲノム情報ビッグバンから読み解く生命圏 GCOE談話会
  • Invited
• [Presentation] Cloning and sequencing of manganese peroxidase gene from Phanerochaete crassa WD1694. (2012)
  • Author(s): Takano M, Yamaguchi M, Sano H, Nakamura M, Shibuya H, Miyazaki Y.
  • Organizer: Lignobiotech II symposium Programme & Abstracts
• [Presentation] シイタケ(Lentinula edodes)の完全なミトコンドリアゲノムと二つのプラスミド様DNA分子の解析 (2012)
  • Author(s): 馬場崎勝彦、宮崎安将
  • Organizer: 日本菌学会大会講演要旨集
• [Presentation] 重力が影響を及ぼすキノコのかたちづくり-遺伝子発現の宇宙環境シミュレーション- (2011)
  • Author(s): 宮崎安将, 砂川政英、東端晃、石岡憲昭、馬場崎勝彦、山崎丘
  • Organizer: 第11回糸状菌分子生物学コンファレンス
  • Place of Presentation: 東京大学(東京)(招待講演)
  • Year and Date: 2011-11-17
• [Presentation] A DASH-type cryptochrome gene from Lentinula edodes (2011)
  • Author(s): Hiroaki Sano, Shinya Kaneko, Masaya Nakamura, Yasumasa Miyazaki
  • Organizer: The 7th International Conference on Mushroom Biology and Mushroom Products
  • Place of Presentation: Arcachon, France
  • Year and Date: 2011-10-06
• [Presentation] Light-stimulative effects on the cultivation of edible mushrooms by using blue LED (2011)
  • Author(s): Yasumasa Miyazaki, Kazuhiko Masuno, Masanori Abe, Hajime Nishizawa, Tetsuo Matsumoto, Sachio Kunitomo, Haruo Sakata, Kimiyoshi Nakamura, Tomoyuki Koyama, Masami Ito, Hiroshi Kazama, Dai Suzuki, Yasushi Obatake, Hiroaki Sano, Masaya Nakamura, Kazuhiro Miyazaki, Yuichi Sakamoto, Shinya Kaneko, Takashi Kamada
  • Organizer: Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products
  • Place of Presentation: Arcachon, France
  • Year and Date: 2011-10-06
• [Presentation] Splicing variants of the phrB gene, encoding a zinc-finger DNA-binding protein interacting with the blue-light photoreceptor phrA, during fruiting body development in the basidiomycetous mushroom Lentinula Edodes (2011)
  • Author(s): Sano H, Kaneko S, Sakamoto Y, Nakamura M, Miyazaki Y
  • Organizer: The abstract of International Union of Microbiological Societies 2011 Congress (IUMS2011), 209p
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-09
• [Presentation] Analyses of ingredients induced by lighting (blue-light LED) for the edible mushroom in Japan (2011)
  • Author(s): Kaneko S, Sakamoto Y, Sano H, Miyazaki K, Abe M, Nishizawa H, Matsumoto T, Kunitomo S, Sakata H, Obatake Y, Nakamura K, Koyama T, Ito M, Kazama H, Suzuki D, Masuno K, Kamada T, Miyazaki Y
  • Organizer: The abstract of International Union of Microbiological Societies 2011 Congress (IUMS2011), 209p
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2011-09-09
• [Presentation] Proteomic analysis using 2-D PAGE and EST databases, during fruiting body formation in the edble mushroom Lentinula edodes (2011)
  • Author(s): Kaneko S, Sakamoto Y, Sano H, Miyazaki K, Abe M, Nishizawa H, Matsumoto T, Kunitomo S, Sakata H, Obatake Y, Nakamura K, Koyama T, ItSano H, Nakazawa T, Kaneko S, Higashibata A, Ishioka N, Nakamura M, Kamada T, Miyazaki Y
  • Organizer: The abstract of Asian Mycological Congress 2011, 355p
  • Place of Presentation: 仁川大学(仁川)
  • Year and Date: 2011-08-07
• [Presentation] シイタケ子実体形成過程における発現タンパク質の解析 (2011)
  • Author(s): 佐野広明、中沢威人、金子真也、東端晃、石岡憲昭、角田光利、鎌田堯、宮崎安将
  • Organizer: 日本農芸化学会2011 年度大会
  • Place of Presentation: 京都女子大学(京都府京都市)
  • Year and Date: 2011-03-26
• [Presentation] シイタケ子実体形成過程における発現タンパク質の解析 (2011)
  • Author(s): 佐野広明、中沢威人、金子真也、東端晃、石岡憲昭、角田光利、鎌田堯、宮崎安将
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学(京都府京都市)
  • Year and Date: 2011-03-26
• [Presentation] Identification of a cryptochrome gene homolog in the basidiomycetous mushroom Lentinula edodes (2010)
  • Author(s): Hiroaki Sano, Shinya Kaneko, Masahide Sunagawa, Kazuhiro Miyazaki, Mitsutoshi Tsunoda, Masaya Nakamura, Yasumasa Miyazaki
  • Organizer: The International Chemical Congress of Pacific Basin Societies (Pacfichem2010)
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-17
• [Presentation] Identification of a cryptochrome gene homolog in the basidiomycetous mushroom Lentinula edodes. (2010)
  • Author(s): Sano H, Kaneko S, Sunagawa M, Miyazaki K, Tsunoda M, Nakamura M, Miyazaki Y.
  • Organizer: The International Chemical Congress of Pacific Basin Societies (Pacfichem2010)
  • Place of Presentation: Honolulu Convention Center (Honolulu)
  • Year and Date: 2010-12-17
• [Presentation] 担子菌ヒラタケの子実体形成過程における重力応答遺伝子群の解析 (2010)
  • Author(s): 宮崎安将、砂川政英、東端晃、石岡憲昭、馬場崎勝彦、山崎丘
  • Organizer: 第10回糸状菌分子生物学コンファレンス
  • Place of Presentation: 広島大学(広島県東広島市)
  • Year and Date: 2010-11-18
• [Presentation] Whole protein analysis by 2-D PAGE during fruiting body formation in Lentinula edodes (2010)
  • Author(s): Hiroaki Sano, Shinya Kaneko, Akira Higashibata, Noriaki Ishioka, Masahide Sunagawa, Yasumasa Miyazaki
  • Organizer: The 9th International Mycological Congress (IMC9)
  • Place of Presentation: Edinburgh, UK
  • Year and Date: 2010-08-04
• [Presentation] Whole protein analysis by 2-D PAGE during fruiting body formation in Lentinla edodes. (2010)
  • Author(s): Sano H, Kaneko S, Higashibata A, Ishioka N, Sunagawa M, Miyazaki Y.
  • Organizer: The 9th International Mycological Congress (IMC9)
  • Place of Presentation: Edinburgh International Conference Centre (Edinburgh)
  • Year and Date: 2010-08-04
• [Presentation] 次世代シーケンサーによるシイタケのゲノム解析とそこから見えること (2010)
  • Author(s): 宮崎安将
  • Organizer: 日本菌学会第54回大会
  • Place of Presentation: 玉川大学(東京都町田市)
  • Year and Date: 2010-05-30
• [Book] 森林総合研究所平成24年版研究成果選集 (2012)
  • Author(s): 宮崎安将、宮崎和弘、中村雅哉、佐野広明、金子真也、鎌田堯、坂本裕一、増野和彦、古 川仁、阿部正範、西澤元、中村公義、小山智 行、風間宏、鈴木大、國友幸夫、阪田春生、小畠靖
  • Publisher: LED 照明を利用したきのこ栽培技術の開発
• [Book] 森林総合研究所 平成 24 年版 研究成果選集 (2012)
  • Author(s): 宮崎安将、宮崎和弘、中村雅哉、佐野広明 、金子真也、 鎌田堯 、坂本裕一、増野和彦、古川仁、 阿部正範、西澤元、中村公義、小山智行、風間宏、鈴木大、 國友幸夫、阪田春生、 小畠靖
  • Total Pages: 2
  • Publisher: LED 照明を利用したきのこ栽培技術の開発
• [Book] BRAINテクノニュース146号 (2011)
  • Author(s): 宮崎安将、金子真也、高野麻理子、中村雅哉、村田仁、馬場崎勝彦
  • Publisher: 農研機構・生研センター
• [Book] 山林8月号 (2011)
  • Author(s): 宮崎安将
  • Publisher: 大日本山林会
• [Book] 社団法人農業電化協会 (2010)
  • Author(s): 宮崎安将
  • Publisher: 人工光源の農林水産分野への応用
• [Book] 人工光源の農林水産分野への応用 (2010)
  • Author(s): 宮崎安将, 他
  • Publisher: 社団法人農業電化協会
• [Remarks] シイタケゲノム・データベース:(独)森林総合研究所遺伝子データベースForestGEN
  • URL: http://forestgen.ffpri.affrc.go.jp/ja/info_le.html
• [Remarks] シイタケゲノム・データベース
  • URL: http://forestgen.ffpri.affrc.go.jp/ja/info_le.html