| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
An endo-.-1,6-galactanase which act on thickening agent arabinogalactan was obtained from Streptomyces avermitilis. The substrate specificity and the crystal structure of the recombinant enzyme were elucidated. To understand the detail mechanism of substrate specificity of the endo-.-1,6-galactanase, the other endo-.-1,6-galactanase which consist from distinctive amino acid sequence was cloned and crystal of the recombinant enzyme was obtained. However, quality of the crystal was not enough to solve structure so that the mechanism of substrate specificity of the endo-.-1,6-galactanase could not be elucidated.
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